MicroRNAs for Virus Pathogenicity and Host Responses, Identified in SARS-CoV-2 Genomes, May Play Roles in Viral-Host Co-Evolution in Putative Zoonotic Host Species.

Lange, S., Arisan, E., Grant, Guy H and Uysal Onganer, P. 2021. MicroRNAs for Virus Pathogenicity and Host Responses, Identified in SARS-CoV-2 Genomes, May Play Roles in Viral-Host Co-Evolution in Putative Zoonotic Host Species. Viruses. 13 (1) 117. https://doi.org/10.3390/v13010117

TitleMicroRNAs for Virus Pathogenicity and Host Responses, Identified in SARS-CoV-2 Genomes, May Play Roles in Viral-Host Co-Evolution in Putative Zoonotic Host Species.
TypeJournal article
AuthorsLange, S., Arisan, E., Grant, Guy H and Uysal Onganer, P.
AbstractOur recent study identified seven key microRNAs (miR-8066, 5197, 3611, 3934-3p, 1307-3p, 3691-3p, 1468-5p) similar between SARS-CoV-2 and the human genome, pointing at miR-related mechanisms in viral entry and the regulatory effects on host immunity. To identify the putative roles of these miRs in zoonosis, we assessed their conservation, compared with humans, in some key wild and domestic animal carriers of zoonotic viruses, including bat, pangolin, pig, cow, rat, and chicken. Out of the seven miRs under study, miR-3611 was the most strongly conserved across all species; miR-5197 was the most conserved in pangolin, pig, cow, bat, and rat; miR-1307 was most strongly conserved in pangolin, pig, cow, bat, and human; miR-3691-3p in pangolin, cow, and human; miR-3934-3p in pig and cow, followed by pangolin and bat; miR-1468 was most conserved in pangolin, pig, and bat; while miR-8066 was most conserved in pangolin and pig. In humans, miR-3611 and miR-1307 were most conserved, while miR-8066, miR-5197, miR-3334-3p and miR-1468 were least conserved, compared with pangolin, pig, cow, and bat. Furthermore, we identified that changes in the miR-5197 nucleotides between pangolin and human can generate three new miRs, with differing tissue distribution in the brain, lung, intestines, lymph nodes, and muscle, and with different downstream regulatory effects on KEGG pathways. This may be of considerable importance as miR-5197 is localized in the spike protein transcript area of the SARS-CoV-2 genome. Our findings may indicate roles for these miRs in viral-host co-evolution in zoonotic hosts, particularly highlighting pangolin, bat, cow, and pig as putative zoonotic carriers, while highlighting the miRs' roles in KEGG pathways linked to viral pathogenicity and host responses in humans. This in silico study paves the way for investigations into the roles of miRs in zoonotic disease.
KeywordsCOVID-19
SARS-CoV-2
co-evolution
microRNA
viral pathogenesis
zoonosis
Article number117
JournalViruses
Journal citation13 (1)
ISSN1999-4915
Year2021
PublisherMDPI
Publisher's version
License
CC BY 4.0
File Access Level
Open (open metadata and files)
Digital Object Identifier (DOI)https://doi.org/10.3390/v13010117
PubMed ID33467206
Publication dates
Published online16 Jan 2021

Related outputs

Prostate Cancer Cell Extracellular Vesicles Increase Mineralisation of Bone Osteoblast Precursor Cells in an In Vitro Model
Lanning, Ben, Webber, J., Uysal Onganer, P., Jiang, W., Clayton, Aled and Dart, D. 2021. Prostate Cancer Cell Extracellular Vesicles Increase Mineralisation of Bone Osteoblast Precursor Cells in an In Vitro Model. Biology. 10 (4), p. e318. https://doi.org/10.3390/biology10040318

Non-coding RNAs in pancreatic ductal adenocarcinoma: New approaches for better diagnosis and therapy
Mortoglou, M., Tabin, Z.K., Arisan, E.D., Kocher, H.M. and Uysal Onganer, P. 2021. Non-coding RNAs in pancreatic ductal adenocarcinoma: New approaches for better diagnosis and therapy. Translational Oncology. 14 (7) 101090. https://doi.org/10.1016/j.tranon.2021.101090

MiR-21 is Required for the Epithelial–Mesenchymal Transition in MDA-MB-231 Breast Cancer Cells
Arisan, E., Rencuzogullari, O., Cieza-Borrella, Clara, Miralles Arenas, Francesc Miralles, Dwek, M., Lange, S. and Uysal Onganer, P. 2021. MiR-21 is Required for the Epithelial–Mesenchymal Transition in MDA-MB-231 Breast Cancer Cells. International Journal of Molecular Sciences. 22 (4), p. e1557 1557. https://doi.org/10.3390/ijms22041557

Peptidylarginine Deiminase Inhibitor Application, Using Cl-Amidine, PAD2, PAD3 and PAD4 Isozyme-Specific Inhibitors in Pancreatic Cancer Cells, Reveals Roles for PAD2 and PAD3 in Cancer Invasion and Modulation of Extracellular Vesicle Signatures
Uysal Onganer, P., D'alessio, S., Mortoglou, M., Kraev, I. and Lange, S. 2021. Peptidylarginine Deiminase Inhibitor Application, Using Cl-Amidine, PAD2, PAD3 and PAD4 Isozyme-Specific Inhibitors in Pancreatic Cancer Cells, Reveals Roles for PAD2 and PAD3 in Cancer Invasion and Modulation of Extracellular Vesicle Signatures. International Journal of Molecular Sciences. 22 (3) 1396. https://doi.org/10.3390/ijms22031396

MicroRNAs for Virus Pathogenicity and Host Responses, Identified in SARS-CoV-2 Genomes, May Play Roles in Viral-Host Co-Evolution in Putative Zoonotic Host Species
Lange, S., Arisan, E.D., Grant, G.H. and Uysal Onganer, P. 2021. MicroRNAs for Virus Pathogenicity and Host Responses, Identified in SARS-CoV-2 Genomes, May Play Roles in Viral-Host Co-Evolution in Putative Zoonotic Host Species. MDPI Viruses. 13 (1), p. 117 10.3390/v13010117 . https://doi.org/10.3390/v13010117

Demonstration of microRNA using in situ hybridisation on formalin fixed paraffin wax samples using conventional oligonucleotide probes: a comparison with the use of locked nucleic acid probes
Warford, A., Rahman, NS, Ribeiro, DA and Uysal Onganer, P. 2020. Demonstration of microRNA using in situ hybridisation on formalin fixed paraffin wax samples using conventional oligonucleotide probes: a comparison with the use of locked nucleic acid probes. British Journal of Biomedical Science. 77 (3), pp. 135-141. https://doi.org/10.1080/09674845.2020.1748913

Specific c-Jun N-Terminal Kinase Inhibitor, JNK-IN-8 Suppresses Mesenchymal Profile of PTX-Resistant MCF-7 Cells through Modulating PI3K/Akt, MAPK and Wnt Signaling Pathways
Ozfiliz Kilbas, P., Sonmez, Ozlem, Uysal Onganer, P., Coker Gurkan, A., Obakan Yerlikaya, P. and Arisan, Elif Damla 2020. Specific c-Jun N-Terminal Kinase Inhibitor, JNK-IN-8 Suppresses Mesenchymal Profile of PTX-Resistant MCF-7 Cells through Modulating PI3K/Akt, MAPK and Wnt Signaling Pathways. Biology. 9 (10), p. e320. https://doi.org/10.3390/biology9100320

Putative Roles for Peptidylarginine Deiminases in COVID-19
Arisan, E.D., Uysal Onganer, P. and Lange, S. 2020. Putative Roles for Peptidylarginine Deiminases in COVID-19. International Journal of Molecular Sciences. 21 (13) 4662. https://doi.org/10.3390/ijms21134662

The Prediction of miRNAs in SARS-CoV-2 Genomes: hsa-miR Databases Identify 7 Key miRs Linked to Host Responses and Virus Pathogenicity-Related KEGG Pathways Significant for Comorbidities
Arisan, E.D., Dart, A., Grant, G.H., Arisan, S., Cuhadaroglu, S., Lange, S. and Uysal Onganer, P. 2020. The Prediction of miRNAs in SARS-CoV-2 Genomes: hsa-miR Databases Identify 7 Key miRs Linked to Host Responses and Virus Pathogenicity-Related KEGG Pathways Significant for Comorbidities. MDPI Viruses. 12 (6) 614. https://doi.org/10.3390/v12060614

Protein Deimination Signatures in Plasma and Plasma-EVs and Protein Deimination in the Brain Vasculature in a Rat Model of Pre-Motor Parkinson’s Disease
Sancandi, M., Uysal Onganer, P., Kraev, I., Mercer, A. and Lange, S. 2020. Protein Deimination Signatures in Plasma and Plasma-EVs and Protein Deimination in the Brain Vasculature in a Rat Model of Pre-Motor Parkinson’s Disease. International Journal of Molecular Sciences. 21 (8) 2743. https://doi.org/10.3390/ijms21082743

The Role of Differentially Expressed miRNAs and Potential miRNA-mRNA Regulatory Network in Prostate Cancer Progression and Metastasis
Arisan, E.D., Freitas, I.L., Pazoki, K., Rahim, S. and Uysal Onganer, P. 2020. The Role of Differentially Expressed miRNAs and Potential miRNA-mRNA Regulatory Network in Prostate Cancer Progression and Metastasis. Biomedical Journal of Technical and Scientific Research. 25 (4), pp. 19382-19387. https://doi.org/10.26717/BJSTR.2020.25.004241

Upregulated Wnt-11 and miR-21 Expression Trigger Epithelial Mesenchymal Transition in Aggressive Prostate Cancer Cells
Arisan, E.D., Rencuzogullari, O., Freitas, I.L., Radzali, S., Keskin, B., Kothari, A., Warford, A. and Uysal Onganer, P. 2020. Upregulated Wnt-11 and miR-21 Expression Trigger Epithelial Mesenchymal Transition in Aggressive Prostate Cancer Cells . Biology. 9 (3) 52. https://doi.org/10.3390/biology9030052

Deiminated Proteins and Extracellular Vesicles as Novel Biomarkers in Pinnipeds: Grey seal (Halichoerus gryptus) and Harbour seal (Phoca vitulina)
Magnadóttir, B., Uysal Onganer, P., Kraev, I., Svansson, V., Skírnisson, K. and Lange, S. 2020. Deiminated Proteins and Extracellular Vesicles as Novel Biomarkers in Pinnipeds: Grey seal (Halichoerus gryptus) and Harbour seal (Phoca vitulina). Biochimie . 171-172, pp. 79-90. https://doi.org/10.1016/j.biochi.2020.02.017

Peptidylarginine Deiminase Isozyme-Specific PAD2, PAD3 and PAD4 Inhibitors Differentially Modulate Extracellular Vesicle Signatures and Cell Invasion in Two Glioblastoma Multiforme Cell Lines
Uysal Onganer, P., MacLatchy, A., Mahmoud, R., Kraev, I., Thompson, P.R., Inal, J.M. and Lange, S. 2020. Peptidylarginine Deiminase Isozyme-Specific PAD2, PAD3 and PAD4 Inhibitors Differentially Modulate Extracellular Vesicle Signatures and Cell Invasion in Two Glioblastoma Multiforme Cell Lines. International Journal of Molecular Sciences. 21 (4) 1495. https://doi.org/10.3390/ijms21041495

Deiminated Proteins and Extracellular Vesicles - Novel Serum Biomarkers in Whales and Orca
Magnadóttir, B., Uysal Onganer, P., Kraev, I., Svansson, V., Hayes, P.M. and Lange, S. 2020. Deiminated Proteins and Extracellular Vesicles - Novel Serum Biomarkers in Whales and Orca. Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics. 34, p. 100676 100676. https://doi.org/10.1016/j.cbd.2020.100676

Extracellular Vesicles, Deiminated Protein Cargo and microRNAs are Novel Serum Biomarkers for Environmental Rearing Temperature in Atlantic Cod (Gadus morhua L.)
Magnadóttir, B., Uysal Onganer, P., Kraev, I., Dodds, A.W., Gudmundsdottir, S. and Lange, S. 2020. Extracellular Vesicles, Deiminated Protein Cargo and microRNAs are Novel Serum Biomarkers for Environmental Rearing Temperature in Atlantic Cod (Gadus morhua L.) . Aquaculture Reports. 16, p. 100245 100245. https://doi.org/10.1016/j.aqrep.2019.100245

Wnt-11 Expression Promotes Invasiveness and Correlates with Survival in Human Pancreatic Ductal Adeno Carcinoma
Dart, D.A., Arisan, D.E., Owen, S., Hao, C., Jiang, W.G. and Uysal Onganer, P. 2019. Wnt-11 Expression Promotes Invasiveness and Correlates with Survival in Human Pancreatic Ductal Adeno Carcinoma. Genes. 10 (11) 921. https://doi.org/10.3390/genes10110921

Post-translational Deimination of Immunological and Metabolic Protein Markers in Plasma and Extracellular Vesicles of Naked Mole-Rat (Heterocephalus glaber)
Pamenter, M.E., Uysal Onganer, P., Huynh, K.W., Kraev, I. and Lange, S. 2019. Post-translational Deimination of Immunological and Metabolic Protein Markers in Plasma and Extracellular Vesicles of Naked Mole-Rat (Heterocephalus glaber). International Journal of Molecular Sciences. 20 (21) 5378. https://doi.org/10.3390/ijms20215378

Peptidylarginine Deiminases Post-translationally deiminate Prohibitin and modulate Extracellular Vesicle Release and microRNAs in Glioblastoma Multiforme
Kosgodage, U., Uysal Onganer, P., Maclatchy, A., Kraev, I., Chatterton, N.P., Nicholas, A.P., Inal, J.M. and Lange, S. 2019. Peptidylarginine Deiminases Post-translationally deiminate Prohibitin and modulate Extracellular Vesicle Release and microRNAs in Glioblastoma Multiforme . International Journal of Molecular Sciences. 20 (1) 103. https://doi.org/10.3390/ijms20010103

Cannabidiol Affects Extracellular Vesicle Release, miR21 and miR126, and Reduces Prohibitin Protein in Glioblastoma Multiforme Cells
Kosgodage, U., Uysal Onganer, P., Maclatchy, A., Mould, R., Nunn, A.V.W., Guy, G.W., Kraev, I., Chatterton, N.P., Thomas, E.L., Inal, J.M., Bell, J.D. and Lange, S. 2019. Cannabidiol Affects Extracellular Vesicle Release, miR21 and miR126, and Reduces Prohibitin Protein in Glioblastoma Multiforme Cells. Translational Oncology. 12 (3), pp. 513-522. https://doi.org/10.1016/j.tranon.2018.12.004

Prostate-specific PTen deletion in mice activates inflammatory microRNA expression pathways in the epithelium early in hyperplasia development
Dart, D.A., Uysal Onganer, P. and Jiang, W.G. 2017. Prostate-specific PTen deletion in mice activates inflammatory microRNA expression pathways in the epithelium early in hyperplasia development. Oncogenesis. 14 (6) 400. https://doi.org/10.1038/s41389-017-0007-5

The interaction of Wnt-11 and signalling cascades in prostate cancer
Koushyar, S., Grant, G.H. and Uysal Onganer, P. 2016. The interaction of Wnt-11 and signalling cascades in prostate cancer. Tumor Biology. 37 (10), pp. 13049-13057. https://doi.org/10.1007/s13277-016-5263-z

Wnt11 in 2011-the regulation and function of a non-canonical Wnt
Uysal Onganer, P. 2012. Wnt11 in 2011-the regulation and function of a non-canonical Wnt. Acta Physiologica. 204.

Distinct expression and activity of GSK-3α and GSK-3β in prostate cancer
Darrington, R.S., Campa, V.M., Walker, M.M., Bengoa-Vergniory, N., Gorrono-Etxebarria, I., Uysal Onganer, P., Kawano, Y., Waxman, J. and Kypta, R.M. 2012. Distinct expression and activity of GSK-3α and GSK-3β in prostate cancer. International Journal of Cancer. 131 (6), p. E872–E883. https://doi.org/10.1002/ijc.27620

Wnt-11 promotes neuroendocrine-like differentiation, survival and migration of prostate cancer cells
Uysal Onganer, P. 2010. Wnt-11 promotes neuroendocrine-like differentiation, survival and migration of prostate cancer cells. Molecular Cancer. 9.

Permalink - https://westminsterresearch.westminster.ac.uk/item/v363x/micrornas-for-virus-pathogenicity-and-host-responses-identified-in-sars-cov-2-genomes-may-play-roles-in-viral-host-co-evolution-in-putative-zoonotic-host-species


Share this
Tweet
Email

Usage statistics

43 total views
5 total downloads
1 views this month
1 downloads this month
These values are for the period from September 2nd 2018, when this repository was created

Export as