Pre‐clinical study of Peripheral Nerve Regeneration using Nerve Guidance Conduits based on Polyhydroxyalkanaotes

Lizarraga Valderrama, L., Ronchi, Giulia, Nigmatullin, Rinat, Fregnan, Federica, Basnett, Pooja, Paxinou, Alexandra, Geuna, Stefano and Roy, I. 2021. Pre‐clinical study of Peripheral Nerve Regeneration using Nerve Guidance Conduits based on Polyhydroxyalkanaotes. Bioengineering & Translational Medicine. e10223. Advanced online publication. https://doi.org/10.1002/btm2.10223

TitlePre‐clinical study of Peripheral Nerve Regeneration using Nerve Guidance Conduits based on Polyhydroxyalkanaotes
TypeJournal article
AuthorsLizarraga Valderrama, L., Ronchi, Giulia, Nigmatullin, Rinat, Fregnan, Federica, Basnett, Pooja, Paxinou, Alexandra, Geuna, Stefano and Roy, I.
Abstract

Nerve guidance conduits (NGCs) are used as an alternative to the “gold standard” nerve autografting, preventing the need for surgical intervention required to harvest autologous nerves. However, the regeneration outcomes achieved with the current NGCs are only comparable with autografting when the gap is short (less than 10 mm). In the present study, we have developed NGCs made from a blend of Polyhydroxyalkanoates, a family of natural resorbable polymers. Hollow NGCs made from a 75:25 Poly(3‐hydroxyoctanoate)/Poly(3‐hydroxybutyrate) blend (PHA‐NGCs) were manufactured using dip‐moulding. These PHA‐NGCs showed appropriate flexibility for peripheral nerve regeneration. In vitro cell studies performed using RT4‐D6P2T rat Schwann cell line confirmed that the material is capable of sustaining cell proliferation and adhesion. PHA‐NGCs were then implanted in vivo to repair 10 mm gaps of the median nerve of female Wistar rats for 12 weeks. Functional evaluation of the regenerated nerve using the grasping test showed that PHA‐NGCs displayed similar motor recovery as the autograft, starting from week 7. Additionally, nerve cross‐sectional area, density and number of myelinated cells, as well as axon diameter, fibre diameter, myelin thickness and g‐ratio obtained using the PHA‐NGCs were found comparable to an autograft. This preclinical data confirmed that the PHA‐NGCs are indeed highly promising candidates for peripheral nerve regeneration.

Article numbere10223
JournalBioengineering & Translational Medicine
ISSN2380-6761
Year2021
PublisherWiley
Publisher's version
License
CC BY 4.0
File Access Level
Open (open metadata and files)
Digital Object Identifier (DOI)https://doi.org/10.1002/btm2.10223
Publication dates
Published online04 May 2021
Licensehttp://onlinelibrary.wiley.com/termsAndConditions#vor

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