Production of calmodulin-tagged proteins in Drosophila Schneider S2 cells: a novel system for antigen production and phage antibody isolation

Congenital macrothrombocytopenia with focal myelofibrosis due to mutations in human G6b-B is rescued in humanized mice
Hofmann, I., Geer, M.J., Vögtle, T., Crispin, A., Campagna, D.R., Barr, A.J., Calicchio, M.L., Heising, S., van Geffen, J.P., Kuijpers, M.J.E., Heemskerk, J.W.M., Eble, J.A., Schmitz-Abe, K., Obeng, E.A., Douglas, M., Freson, K., Pondarré, C., Favier, R., Jarvis, G.E., Markianos, K., Turro, E., Ouwehand, W.H., Mazharian, A., Fleming, M.D. and Senis, Y. 2018. Congenital macrothrombocytopenia with focal myelofibrosis due to mutations in human G6b-B is rescued in humanized mice. Blood. 132, pp. 1399-1412. https://doi.org/10.1182/blood-2017-08-802769

Molecular characterization of the variable domains of an αIIbβ3-specific immunoglobulin M κ platelet cold agglutinin in a follicular lymphoma patient with treatment refractory autoimmune thrombocytopenia: idiotypic overlap between αIIbβ3 integrin antibodies
Jennings, N.S., Harmer, I., Campbell, K., Stafford, P., Smith, G.A., Metcalfe, P., Benton, M.A., Marsh, J.C.W. and Ouwehand, W.H. 2007. Molecular characterization of the variable domains of an αIIbβ3-specific immunoglobulin M κ platelet cold agglutinin in a follicular lymphoma patient with treatment refractory autoimmune thrombocytopenia: idiotypic overlap between αIIbβ3 integrin antibodies. Transfusion. 47 (3), pp. 499-510. https://doi.org/10.1111/j.1537-2995.2006.01142.x

Definition of novel GP6 polymorphisms and major difference in haplotype frequencies between populations by a combination of in-depth exon resequencing and genotyping with tag single nucleotide polymorphisms
Watkins, N.A., O'Connor, M.N., Rankin, A., Jennings, N.S., Wilson, E., Harmer, I., Davies, L., Smethurst, P.A., Dudbridge, F., Farndale, R.W. and Ouwehand, W.H. 2006. Definition of novel GP6 polymorphisms and major difference in haplotype frequencies between populations by a combination of in-depth exon resequencing and genotyping with tag single nucleotide polymorphisms. Journal of Thrombosis and Haemostasis. 4 (6), pp. 1197-1205. https://doi.org/10.1111/j.1538-7836.2006.01937.x

Platelet integrin α 2 I-domain specific antibodies produced via domain specific DNA vaccination combined with variable gene phage display
Hughes, D.L., Stafford, P., Hamaia, S.W., Harmer, I., Schoolmeester, A., Deckmyn, H., Farndale, R.W., Ouwehand, W.H. and Watkins, N.A. 2005. Platelet integrin α 2 I-domain specific antibodies produced via domain specific DNA vaccination combined with variable gene phage display. Thrombosis and Haemostasis. 94 (16), pp. 1318-1326. https://doi.org/10.1160/TH05-06-0410

The role of antigen in the selection of the human V3-23 immunoglobulin heavy chain variable region gene
Mackworth-Young, C.G., Harmer, I. and Mageed, R.A. 2003. The role of antigen in the selection of the human V3-23 immunoglobulin heavy chain variable region gene. Clinical and Experimental Immunology. 134 (3), pp. 420-425. https://doi.org/10.1111/j.1365-2249.2003.02319.x

Rearrangement of the human heavy chain variable region gene V3-23 in transgenic mice generates antibodies reactive with a range of antigens on the basis of VHCDR3 and residues intrinsic to the heavy chain variable region
Mageed, R.A., Harmer, I., Wynn, S.L., Moyes, S.P., Maziak, B.R., Bruggemann, M. and Mackworth-Young, C.G. 2001. Rearrangement of the human heavy chain variable region gene V3-23 in transgenic mice generates antibodies reactive with a range of antigens on the basis of VHCDR3 and residues intrinsic to the heavy chain variable region. Clinical and Experimental Immunology. 123 (1), pp. 1-8. https://doi.org/10.1046/j.1365-2249.2001.01380.x

The BH1 idiotype defines a population of anticardiolipin antibodies closely associated with the antiphospholipid syndrome
Mason, A.N., Harmer, I. and Mackworth-Young, C.G. 1999. The BH1 idiotype defines a population of anticardiolipin antibodies closely associated with the antiphospholipid syndrome. Lupus. 8 (3), pp. 234-239. https://doi.org/10.1191/096120399678847687

Chimaeric monoclonal antibodies encoded by the human VH26 gene from naive transgenic mice display a wide range of antigen binding specificities
Harmer, I., Mageed, R.A., Kaminski, A., Charles, P., Bruggemann, M. and Mackworth-Young, C.G. 1996. Chimaeric monoclonal antibodies encoded by the human VH26 gene from naive transgenic mice display a wide range of antigen binding specificities. Immunology. 88 (2), pp. 174-182. https://doi.org/10.1111/j.1365-2567.1996.tb00002.x

Phospholipid binding specificities and idiotype expression of hybridoma derived monoclonal autoantibodies from splenic cells of patients with systemic lupus erythematosus
Ravirajan, C.T., Harmer, I., McNally, T., Hohmann, A., Mackworth-Young, C.G. and Isenberg D, A. 1995. Phospholipid binding specificities and idiotype expression of hybridoma derived monoclonal autoantibodies from splenic cells of patients with systemic lupus erythematosus. Annals of the Rheumatic Diseases. 54 (6), pp. 471-476. https://doi.org/10.1136/ard.54.6.471

Endothelium-derived haemostatic factors and the antiphospholipid syndrome
Mackworth-Young, C.G., Andreotti, F., Harmer, I., Loizou, S., Pottinger, B.E., Pearson, J.D., Maseri, A. and Walport M, J. 1995. Endothelium-derived haemostatic factors and the antiphospholipid syndrome. British Journal of Rheumatology. 34, pp. 201-206.

A human monoclonal antiphospholipid antibody that is representative of serum antibodies and is germline encoded
Harmer, I., Loizou, S., Thompson, K., So, A.K., Walport M, J., Mackworth-Young, C.G. and A. K. So 1995. A human monoclonal antiphospholipid antibody that is representative of serum antibodies and is germline encoded. Arthritis and Rheumatism. 38, pp. 1068-1076. https://doi.org/10.1002/art.1780380808

The FITC-anti-FITC system is a sensitive alternative to biotin-streptavidin in ELISA
Harmer, I. and Samuel, D. 1989. The FITC-anti-FITC system is a sensitive alternative to biotin-streptavidin in ELISA. Journal of Immunological Methods. 122 (1), pp. 115-121. https://doi.org/10.1016/0022-1759(89)90341-4

Structural and idiotypic relationships between monoclonal antibodies specific for IgG1 (Fc) subclass epitopes
Harmer, I. and Jefferis, R. 1988. Structural and idiotypic relationships between monoclonal antibodies specific for IgG1 (Fc) subclass epitopes. Biochemical Society Transactions. 16 (5), pp. 747-8. https://doi.org/10.1042/bst0160747a