Cancer-associated glycoforms of gelatinase B exhibit a decreased level of binding to galectin-3

Fry, S., Van den Steen, P.E., Royle, L., Wormald, M.R., Leathem, A., Opdenakker, G., McDonnell, J.M., Dwek, R.A. and Rudd, P.M. 2006. Cancer-associated glycoforms of gelatinase B exhibit a decreased level of binding to galectin-3. Biochemistry. 45 (51), pp. 15249-15258. https://doi.org/10.1021/bi061254l

TitleCancer-associated glycoforms of gelatinase B exhibit a decreased level of binding to galectin-3
AuthorsFry, S., Van den Steen, P.E., Royle, L., Wormald, M.R., Leathem, A., Opdenakker, G., McDonnell, J.M., Dwek, R.A. and Rudd, P.M.
Abstract

Gelatinase B (MMP-9) and galectin-3 are widely known to participate in tumor cell invasion and metastasis. Glycans derived from MMP-9 expressed in MCF-7 breast cancer and THP-1 myeloid leukemia cells were compared with those from MMP-9 expressed in natural neutrophils. The many O-linked glycans of neutrophil gelatinase B presented a cluster of mainly galactosylated core II structures, 46% of which were ligands for galectin-3; 11% contained two to three N-acetyllactosamine repeating units that are high-affinity ligands for the lectin. The glycan epitopes thus provide MMP-9 with both high-affinity and (presumably) high-avidity interactions with galectin-3. In contrast, the O-glycans released from MMP-9 expressed in MCF-7 and THP-1 cells were predominantly sialylated core I structures. Only 10% of MCF-7 and THP-1 gelatinase B O-glycans were ligands for galectin-3 and contained only a maximum single N-acetyllactosamine repeat. Consistent with the glycan analysis, surface plasmon resonance binding assays indicated that the cancer-associated glycoforms of MMP-9 bound galectin-3 with an affinity and avidity significantly reduced compared with those of the natural neutrophil MMP-9. Galectin-3 exists as a multimer that also binds laminin, providing a means of localizing neutrophil MMP-9 in the extracellular matrix (ECM). The analytical data presented here suggest that MMP-9 glycoforms secreted by tumor cells are unlikely to be tethered at the site of secretion, thus promoting more extensive cleavage of the ECM and providing a rationale for the contribution that gelatinase B makes to cancer cell metastasis.

JournalBiochemistry
Journal citation45 (51), pp. 15249-15258
ISSN0006-2960
Year2006
PublisherAmerican Chemical Society
Digital Object Identifier (DOI)https://doi.org/10.1021/bi061254l
Publication dates
Published2006

Related outputs

Cadherin-5: a biomarker for metastatic breast cancer with optimum efficacy in oestrogen receptor-positive breast cancers with vascular invasion
Fry, S., Robertson, C.E., Swann, R. and Dwek, M. 2016. Cadherin-5: a biomarker for metastatic breast cancer with optimum efficacy in oestrogen receptor-positive breast cancers with vascular invasion. British Journal of Cancer. 114, pp. 1019-1026. https://doi.org/10.1038/bjc.2016.66

A targeted glycoproteomic approach identifies cadherin-5 as a novel biomarker of metastatic breast cancer
Fry, S., Sinclair, J., Timms, J.F., Leathem, A. and Dwek, M. 2013. A targeted glycoproteomic approach identifies cadherin-5 as a novel biomarker of metastatic breast cancer. Cancer Letters. 328 (2), pp. 335-344. https://doi.org/10.1016/j.canlet.2012.10.011

The DietCompLyf study: a prospective cohort study of breast cancer survival and phytoestrogen consumption
Swann, R., Perkins, K.A., Velentzis, L.S., Ciria, C., Dutton, S., Mulligan, A.A., Woodside, J., Cantwell, M.M., Leathem, A., Robertson, C.E. and Dwek, M. 2013. The DietCompLyf study: a prospective cohort study of breast cancer survival and phytoestrogen consumption. Maturitas. 75 (3), pp. 232-240. https://doi.org/10.1016/j.maturitas.2013.03.018

The DietCompLyf study: a prospective longitudinal study of breast cancer survival
Swann, R., Perkins, A., Velentzis, L.S., Mulligan, A.M., Woodside, J., Cantwell, M.M., Dutton, S., Leathem, A., Robertson, C.E. and Dwek, M. 2012. The DietCompLyf study: a prospective longitudinal study of breast cancer survival. European Journal of Cancer. 48 (5), p. S216. https://doi.org/10.1016/S0959-8049(12)71525-3

DietCompLyf study: a multi-centre UK study on breast cancer. What are the dietary and lifestyle changes following diagnosis?
Perkins, A., Swann, R., Woodside, J., Robertson, C.E., Dutton, S., Mulligan, A.M., Velentzis, L.S., Keshtgar, M.R., Leathem, A. and Dwek, M. 2012. DietCompLyf study: a multi-centre UK study on breast cancer. What are the dietary and lifestyle changes following diagnosis? European Journal of Cancer. 48 (5), p. S282. https://doi.org/10.1016/S0959-8049(12)71766-5

Lectin array based strategies for identifying metastasis-associated changes in glycosylation
Fry, S., Afrough, B., Leathem, A. and Dwek, M. 2012. Lectin array based strategies for identifying metastasis-associated changes in glycosylation. Methods in Molecular Biology. 878, pp. 267-272. https://doi.org/10.1007/978-1-61779-854-2_18

Association of serum anti-Tn IgM with breast cancer recurrence
Afrough, B., Fry, S., Lomax-Browne, H., Perkins, A., Leathem, A. and Dwek, M. 2011. Association of serum anti-Tn IgM with breast cancer recurrence. Immunology. 135 (Supp.1), p. 153. https://doi.org/10.1111/j.1365-2567.2011.03534.x

Significant changes in dietary intake and supplement use after breast cancer diagnosis in a UK multicentre study
Velentzis, L.S., Keshtgar, M.R., Woodside, J., Leathem, A., Titcomb, A., Perkins, A., Mazurowska, M., Anderson, V., Wardell, K. and Cantwell, M.M. 2011. Significant changes in dietary intake and supplement use after breast cancer diagnosis in a UK multicentre study. Breast Cancer Research and Treatment. 128 (2), pp. 473-482. https://doi.org/10.1007/s10549-010-1238-8

Abstract B76 - An overview of the DietCompLyf study - a multicentre UK study to evaluate the role of diet, lifestyle and complementary medicine use on breast cancer recurrence
Swann, R., Perkins, A., Dahya, P., Woodside, J., Dutton, S., Robertson, C.E., Velentzis, L.S., Leathem, A. and Dwek, M. 2011. Abstract B76 - An overview of the DietCompLyf study - a multicentre UK study to evaluate the role of diet, lifestyle and complementary medicine use on breast cancer recurrence. National Cancer Research Institute (NCRI) Cancer Conference 2011. BT Convention Centre, Liverpool, UK 06 - 09 Nov 2011

Lectin microarray profiling of metastatic breast cancers
Fry, S., Afrough, B., Lomax-Browne, H., Timms, J.F., Velentzis, L.S. and Leathem, A. 2011. Lectin microarray profiling of metastatic breast cancers. Glycobiology. 21 (8), pp. 1060-1070. https://doi.org/10.1093/glycob/cwr045

Abstract LB14 - Lectin microarray profilling of metastatic breast cancers
Fry, S., Afrough, B., Lomax-Browne, H., Timms, J.F., Velentzis, L.S. and Dwek, M. 2011. Abstract LB14 - Lectin microarray profilling of metastatic breast cancers. National Cancer Research Institute (NCRI) Cancer Conference 2011. BT Convention Centre, Liverpool, UK 06 - 09 Nov 2011

A sensitive assay to measure biomarker glycosylation demonstrates increased fucosylation of prostate specific antigen (PSA) in patients with prostate cancer compared with benign prostatic hyperplasia
Dwek, M., Jenks, A. and Leathem, A. 2010. A sensitive assay to measure biomarker glycosylation demonstrates increased fucosylation of prostate specific antigen (PSA) in patients with prostate cancer compared with benign prostatic hyperplasia. Clinica Chimica Acta. 411 (23-24), pp. 1935-1939. https://doi.org/10.1016/j.cca.2010.08.009

UEA-1 enables discrimination between prostate specific antigen from patients with prostate cancer and benign prostatic hyperplasia
Dwek, M., Jenks, A. and Leathem, A. 2009. UEA-1 enables discrimination between prostate specific antigen from patients with prostate cancer and benign prostatic hyperplasia. Glycobiology. 19 (11), p. 1318. https://doi.org/10.1093/glycob/cwp135

Altered glycosylation of proteins in cancer: what is the potential for new anti-tumour strategies
Brooks, S.A., Carter, T.M., Royle, L., Harvey, D.J., Fry, S., Kinch, C., Dwek, R.A. and Rudd, P.M. 2008. Altered glycosylation of proteins in cancer: what is the potential for new anti-tumour strategies. Anti-Cancer Agents in Medicinal Chemistry. 8 (1), pp. 2-21.

The hemopexin and O-glycosylated domains tune gelatinase B/MMP-9 bioavailability via inhibition and binding to cargo receptors
Van den Steen, P.E., Van Aelst, I., Hvidberg, V., Piccard, H., Fiten, P., Jacobsen, C., Moestrup, S.K., Fry, S., Royle, L., Wormald, M.R., Wallis, R., Rudd, P.M., Dwek, R.A. and Opdenakker, G. 2006. The hemopexin and O-glycosylated domains tune gelatinase B/MMP-9 bioavailability via inhibition and binding to cargo receptors. Journal of Biological Chemistry. 281 (27), pp. 18626-18637.

Differential glycosylation of gelatinase B from neutrophils and breast cancer cells
Fry, S., Van den Steen, P.E., Royle, L., Wormald, M.R., Leathem, A., Opdenakker, G., Rudd, P.M. and Dwek, R.A. 2005. Differential glycosylation of gelatinase B from neutrophils and breast cancer cells. Advances in Experimental Medicine and Biology. 564, pp. 103-112. https://doi.org/10.1007/0-387-25515-X_18

Permalink - https://westminsterresearch.westminster.ac.uk/item/9254w/cancer-associated-glycoforms-of-gelatinase-b-exhibit-a-decreased-level-of-binding-to-galectin-3


Share this

Usage statistics

125 total views
0 total downloads
These values cover views and downloads from WestminsterResearch and are for the period from September 2nd 2018, when this repository was created.