Intracellular expression of the truncated extracellular domain of c-erbB-3/HER3

Srinivasan, R., Leverton, K., Sheldon, H., Hurst, H., Sarraf, C. and Gullick, W. 2001. Intracellular expression of the truncated extracellular domain of c-erbB-3/HER3. Cellular Signalling. 13 (5), pp. 321-330.

TitleIntracellular expression of the truncated extracellular domain of c-erbB-3/HER3
AuthorsSrinivasan, R., Leverton, K., Sheldon, H., Hurst, H., Sarraf, C. and Gullick, W.
Abstract

The ERBB3 gene is expressed as a 6.2- and a 1.4-kb transcript. The former encodes the full-length transmembrane protein and the latter a truncated extracellular fragment consisting of 140 amino acids of the c-erbB-3 protein followed by 43 unique residues. We have examined the expression of the two ERBB3 transcripts by Northern blotting in cancer cell lines and normal human fetal and adult tissues. We expressed the truncated receptor fragment and showed that it was glycosylated, probably with a single N-linked complex sugar chain, and that the protein was a 58-kDa disulphide-linked dimer. We were able to crosslink iodinated neuregulin (NRG)-1β to the full-length solubilised receptor but not to the truncated dimeric protein. Using Western blot analysis, the truncated protein was shown to be present in cell lysates and, using immunoelectron microscopy, in vesicular structures within cells and associated with the plasma cell membrane

JournalCellular Signalling
Journal citation13 (5), pp. 321-330
ISSN0898-6568
YearMay 2001
Digital Object Identifier (DOI)doi:10.1016/S0898-6568(01)00155-3
Publication dates
PublishedMay 2001

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