Construction of chimeric Arctic-like rabies virus glycoproteins rescues pseudovirus titre to permit use in serological studies

Bentley, E., Ali, R., Horton, D., Banyard, A.C. and Wright, E. 2014. Construction of chimeric Arctic-like rabies virus glycoproteins rescues pseudovirus titre to permit use in serological studies. Society for General Microbiology Spring Conference. Liverpool, UK Apr 2014

TitleConstruction of chimeric Arctic-like rabies virus glycoproteins rescues pseudovirus titre to permit use in serological studies
AuthorsBentley, E., Ali, R., Horton, D., Banyard, A.C. and Wright, E.
Abstract

Arctic-like rabies (AL RABV) viruses, a lineage of RABV circulating widely in the Middle East and Asia, have distinct antigenic and genetic characteristics. Similar to other members of the RABV species, they cause a zoonotic disease ultimately leading to death of infected patients. RABV glycoprotein (G) lentiviral pseudovirus (PV) has shown to be a highly sensitive and specific surrogate to live virus in neutralisation assays. However, using wildtype AL RABV G failed to generate infectious PV. Thus, we investigated the generation of chimeric AL RABV G to increase the PV titre. Chimeras were constructed by splicing the ecto- and transmembrane domains of four AL RABV G strains with the cytoplasmic domains of vesicular stomatitis virus (VSV) or RABV challenge virus standard-11 (CVS-11) G. PV produced expressing wildtype or chimeric G revealed chimeric AL RABV with VSV G but not CVS-11 significantly increased PV titres. Hence we have sought to undertake neutralisation studies with the AL RABV VSV G chimeras, testing the efficacy of current vaccines and antivirals against this RABV subset.

Year2014
ConferenceSociety for General Microbiology Spring Conference

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Construction of chimeric Arctic-like rabies virus glycoproteins improves pseudotyped virus titre to permit use in serological studies
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The construction of chimeric rabies virus glycoproteins rescues Arctic-like rabies pseudovirus production
Bentley, E., Ali, R., Horton, D.L., Banyard, A.C. and Wright, E. 2013. The construction of chimeric rabies virus glycoproteins rescues Arctic-like rabies pseudovirus production. Pseudotype viruses: applications and troubleshooting. London, UK October 2013

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