Poly(3-Hydroxybutyrate), P(3HB) production and its biomedical applications

Akaraonye, E. 2011. Poly(3-Hydroxybutyrate), P(3HB) production and its biomedical applications. PhD thesis University of Westminster Faculty of Science and Technology

TitlePoly(3-Hydroxybutyrate), P(3HB) production and its biomedical applications
TypePhD thesis
AuthorsAkaraonye, E.
Abstract

In this project enhancement of poly(3-hydroxybutyrate), P(3HB) production using a Gram positive bacteria, Bacillus cereus SPV and sucrose as the main carbon source was successfully achieved. Different modes of fermentation including shaken flask, batch, fedbatch and two-stage fermentation were investigated in the study. A modified G-medium was formulated and used throughout the study. Potassium and sulphate were identified as the main limiting factor for P(3HB) accumulation in Bacillus cereus SPV. By limiting the potassium phosphate concentration to 0.5 g/L K2HPO4 in the production medium, the dry cell weight, P(3HB) yield and P(3HB) concentration improved to, 7.21 g/L, 82 % dcw and 5.95 g/L respectively (i.e. 236, 115.8 and 830 % increase in dry cell weight, P(3HB) yield and P(3HB) concentration respectively). In addition, economic production of P(3HB) using agricultural/industrial waste (molasses) as the main carbon substrate was achieved. The study was also carried out in both shaken flask and 2L fermenter. The maximum P(3HB) yield achieved was 61.07 % dcw in 1L shaken flask and 51.37 % dcw in 2L fermenter.

A novel wet cell PHA extraction was successfully developed in this project leading to high purity of the PHA produced with reduced crystallinity and efficient recovery. This is expected to save time, cost and enhanced continuous PHA production.

Furthermore, a novel inexpensive and sustainable ‘compression moulding/particulate leaching’ technique for tissue engineering scaffold fabrication was developed. The novel technique enabled the production of biodegradable composite scaffolds of P(3HB)/microfibrillated cellulose and magnetic P(3HB) nanocomposite for possible applications in cartilage and bone tissue engineering, respectively. Detailed studies on the 2D and 3D composites showed that the inclusion of microfibrillated cellulose into the P(3HB) matrix enhanced the mechanical properties, hydrophilicity, introduced microtopography features, enhanced surface chemistry and biocompatibility of the composite material while inclusion of magnetic nanoparticles and ferrofluid, in addition to the above features added magnetic properties and microhardness to the composite materials.

A unique controlled drug delivery system was developed with potential application in multiple drug delivery. The release study confirmed that the delivery system was able to control the release of BSA, a model protein.

Finally, composite magnetic microspheres were also produced and characterised for their efficient use in the delivery of cancer drug and analyses performed showed that the composite constructs have superparamagnetic properties which would be useful for targeted delivery.

Year2011
FileEverest_Chukwuemeka_AKARAONYE_2011.pdf
Publication dates
Completed2011

Related outputs

Poly(3-hydroxybutyrate) production by Bacillus cereus SPV using sugarcane molasses as the main carbon source
Akaraonye, E., Moreno, C., Knowles, J.C., Keshavarz, T. and Roy, I. 2012. Poly(3-hydroxybutyrate) production by Bacillus cereus SPV using sugarcane molasses as the main carbon source. Biotechnology Journal. 7 (2), pp. 293-303. doi:10.1002/biot.201100122

Production of polyhydroxyalkanoates and their medical applications
Roy, I., Akaraonye, E., Francis, L., Rai, R., Basnett, P. and Keshavarz, T. 2011. Production of polyhydroxyalkanoates and their medical applications. 7th International Conference on Polymer and Textile Biotechnology. Milan, Italy 2nd - 4th March 2011

Production of polyhydroxyalkanoates and their biomedical applications
Roy, I., Akaraonye, E., Francis, L., Rai, R., Basnett, P. and Keshavarz, T. 2011. Production of polyhydroxyalkanoates and their biomedical applications. Euro BioMat 2011 - European Symposium on Biomaterials and Related Areas. Jena, Germany

Production of SCL and MCL polyhydroxyalkanoates and their applications
Roy, I., Akaraonye, E., Francis, L., Rai, R. and Boccaccini, A.R. 2010. Production of SCL and MCL polyhydroxyalkanoates and their applications. 12th International Symposium on Biodegradable Polyesters. Stuttgart, Germany.

Enhanced and economical production of poly(3-hydroxybutyrate), a biodegradable polymer and its applications
Akaraonye, E., Safarikova, M., Keshavarz, T. and Roy, I. 2010. Enhanced and economical production of poly(3-hydroxybutyrate), a biodegradable polymer and its applications. 12th International Symposium on Biodegradable Polyesters. Stuttgart, Germany.

Production of polyhydroxyalkanoates: the future green materials of choice
Akaraonye, E., Keshavarz, T. and Roy, I. 2010. Production of polyhydroxyalkanoates: the future green materials of choice. Journal of Chemical Technology and Biotechnology. 85 (6), pp. 732-743. doi:10.1002/jctb.2392

P(3HB) production by Bacillus cereus SPV using sucrose as a carbon source
Akaraonye, E., Keshavarz, T. and Roy, I. 2009. P(3HB) production by Bacillus cereus SPV using sucrose as a carbon source. in: Kiekens, P., Nierstrasz, V. and Lenting, H.B.M. (ed.) Proceedings of the international conference on textile and polymer biotechnology 6th Het Pand, Ghent, Belgium 23-25 September 2009 Ghent University. Department of Textiles Ghent.

Permalink - https://westminsterresearch.westminster.ac.uk/item/96500/poly-3-hydroxybutyrate-p-3hb-production-and-its-biomedical-applications


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