Mussel Inspired Chemistry and Bacteria Derived Polymers for Oral Mucosal Adhesion and Drug Delivery.

Owji, Nazanin, Mandakhbayar, Nandin, Gregory, David A, Marcello, E., Kim, Hae-Won, Roy, Ipsita and Knowles, Jonathan C 2021. Mussel Inspired Chemistry and Bacteria Derived Polymers for Oral Mucosal Adhesion and Drug Delivery. Frontiers in Bioengineering and Biotechnology. 9 663764. https://doi.org/10.3389/fbioe.2021.663764

TitleMussel Inspired Chemistry and Bacteria Derived Polymers for Oral Mucosal Adhesion and Drug Delivery.
TypeJournal article
AuthorsOwji, Nazanin, Mandakhbayar, Nandin, Gregory, David A, Marcello, E., Kim, Hae-Won, Roy, Ipsita and Knowles, Jonathan C
AbstractUlceration of the oral mucosa is common, can arise at any age and as a consequence of the pain lessens enjoyment and quality of life. Current treatment options often involve the use of topical corticosteroids with poor drug delivery systems and inadequate contact time. In order to achieve local controlled delivery to the lesion with optimal adhesion, we utilized a simple polydopamine chemistry technique inspired by mussels to replicate their adhesive functionality. This was coupled with production of a group of naturally produced polymers, known as polyhydroxyalkanoates (PHA) as the delivery system. Initial work focused on the synthesis of PHA using CH50; once synthesized and extracted from the bacteria, the PHAs were solvent processed into films. Polydopamine coating was subsequently achieved by immersing the solvent cast film in a polymerized dopamine solution. Fourier Transform Infrared Spectroscopy (FTIR) spectroscopy confirmed functionalization of the PHA films via the presence of amine groups. Further characterization of the samples was carried out via surface energy measurements and Scanning Electron Microscopy (SEM) micrographs for surface topography. An adhesion test via reverse compression testing directly assessed adhesive properties and revealed an increase in polydopamine coated samples. To further identify the effect of surface coating, LIVE/DEAD imaging and Alamar Blue metabolic activity evaluated attachment and proliferation of fibroblasts on the biofilm surfaces, with higher cell growth in favor of the coated samples. Finally, biocompatibility was investigated in a rat model where the polydopamine coated PHA showed less inflammatory response over time compared to uncoated samples with sign of neovascularization. In conclusion, this simple mussel inspired polydopamine chemistry introduces a step change in bio-surface functionalization and holds great promise for the treatment of oral conditions. [Abstract copyright: Copyright © 2021 Owji, Mandakhbayar, Gregory, Marcello, Kim, Roy and Knowles.]
Keywordsbiomimetic
drug delivery
oral mucosa
polydopamine chemistry
polyhydroxyalkanoates
surface functionalization
Article number663764
JournalFrontiers in Bioengineering and Biotechnology
Journal citation9
ISSN2296-4185
Year2021
PublisherFrontiers
Publisher's version
License
CC BY 4.0
File Access Level
Open (open metadata and files)
Digital Object Identifier (DOI)https://doi.org/10.3389/fbioe.2021.663764
PubMed ID34026742
Publication dates
Published online05 May 2021

Related outputs

Antibiotic-free PHA-based antibacterial materials for bone regeneration
Marcello, E. 2020. Antibiotic-free PHA-based antibacterial materials for bone regeneration. PhD thesis University of Westminster Life Sciences https://doi.org/10.34737/v11yy

Permalink - https://westminsterresearch.westminster.ac.uk/item/v54y6/mussel-inspired-chemistry-and-bacteria-derived-polymers-for-oral-mucosal-adhesion-and-drug-delivery


Share this

Usage statistics

51 total views
66 total downloads
These values cover views and downloads from WestminsterResearch and are for the period from September 2nd 2018, when this repository was created.