Transcriptional profiling of zebrafish identifies host factors controlling susceptibility to Shigella flexneri

Torraca, V., White, R., Sealy, I., Mazon-Moya, M., Duggan, G., Willis, A., Busch-Nentwich, E. and Mostowy, S. 2024. Transcriptional profiling of zebrafish identifies host factors controlling susceptibility to Shigella flexneri. Disease Models and Mechanisms. 17 (1) dmm050431. https://doi.org/10.1242/dmm.050431

TitleTranscriptional profiling of zebrafish identifies host factors controlling susceptibility to Shigella flexneri
TypeJournal article
AuthorsTorraca, V., White, R., Sealy, I., Mazon-Moya, M., Duggan, G., Willis, A., Busch-Nentwich, E. and Mostowy, S.
AbstractShigella flexneri is a human-adapted pathovar of Escherichia coli that can invade the intestinal epithelium, causing inflammation and bacillary dysentery. Although an important human pathogen, the host response to S. flexneri has not been fully described. Zebrafish larvae represent a valuable model to study human infections in vivo. Here we use a Shigella-zebrafish infection model to generate mRNA expression profiles of host response to Shigella infection at the whole animal level. Immune response-related processes dominate the signature of early Shigella infection (6 hours post-infection). Consistent with its clearance from the host, the signature of late Shigella infection (24 hours post-infection) is significantly changed, and only a small set of immune-related genes remain differentially expressed, including acod1 and gpr84. Using mutant lines generated by ENU, CRISPR mutagenesis and F0 Crispants, we show that acod1- and gpr84-deficient larvae are more susceptible to Shigella infection. Together, these results highlight the power of zebrafish to model infection by bacterial pathogens and reveal the mRNA expression of the early (acutely infected) and late (clearing) host response to Shigella infection.
KeywordsGeneral Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology (miscellaneous)
Medicine (miscellaneous)
Neuroscience (miscellaneous)
Article numberdmm050431
JournalDisease Models and Mechanisms
Journal citation17 (1)
ISSN1754-8403
Year2024
PublisherThe Company of Biologists
Publisher's version
License
CC BY 4.0
File Access Level
Open (open metadata and files)
Digital Object Identifier (DOI)https://doi.org/10.1242/dmm.050431
Publication dates
Published online22 Dec 2023
Published in print2024
ProjectH2020-MSCA-IF-2015 – 700088
100229/Z/12/Z
WT097411MA
NA
772853 - ENTRAPMENT
FunderEuropean Commission
Wellcome Trust
Wellcome Trust
Lister Institute of Preventive Medicine
Wellcome Trust
European Research Council

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