Liu, S., Shi-Wen, X., Blumbach, K., Eastwood, M., Denton, C.P., Eckes, B., Krieg, T., Abraham, D.J. and Leask, A. 2010. Expression of integrin β1 by fibroblasts is required for tissue repair in vivo. Journal of Cell Science. 123 (21), pp. 3674-3682. https://doi.org/10.1242/jcs.070672
| Title | Expression of integrin β1 by fibroblasts is required for tissue repair in vivo |
|---|---|
| Authors | Liu, S., Shi-Wen, X., Blumbach, K., Eastwood, M., Denton, C.P., Eckes, B., Krieg, T., Abraham, D.J. and Leask, A. |
| Abstract | In tissue repair, fibroblasts migrate into the wound to produce and remodel extracellular matrix (ECM). Integrins are believed to be crucial for tissue repair, but their tissue-specific role in this process is poorly understood. Here, we show that mice containing a fibroblast-specific deletion of integrin β1 exhibit delayed cutaneous wound closure and less granulation tissue formation, including reduced production of new ECM and reduced expression of α-smooth muscle actin (α-SMA). Integrin-β1-deficient fibroblasts showed reduced expression of type I collagen and connective tissue growth factor, and failed to differentiate into myofibroblasts as a result of reduced α-SMA stress fiber formation. Loss of integrin β1 in adult fibroblasts reduced their ability to adhere to, to spread on and to contract ECM. Within stressed collagen matrices, integrin-β1-deficient fibroblasts showed reduced activation of latent TGFβ. Addition of active TGFβ alleviated the phenotype of integrin-β1-deficient mice. Thus integrin β1 is essential for normal wound healing, where it acts, at least in part, through a TGFβ-dependent mechanism in vivo. |
| Journal | Journal of Cell Science |
| Journal citation | 123 (21), pp. 3674-3682 |
| ISSN | 0021-9533 |
| Year | Nov 2010 |
| Publisher | The Company of Biologists Ltd. |
| Digital Object Identifier (DOI) | https://doi.org/10.1242/jcs.070672 |
| Publication dates | |
| Published | Nov 2010 |