Expression of integrin β1 by fibroblasts is required for tissue repair in vivo

Liu, S., Shi-Wen, X., Blumbach, K., Eastwood, M., Denton, C.P., Eckes, B., Krieg, T., Abraham, D.J. and Leask, A. 2010. Expression of integrin β1 by fibroblasts is required for tissue repair in vivo. Journal of Cell Science. 123 (21), pp. 3674-3682. https://doi.org/10.1242/jcs.070672

TitleExpression of integrin β1 by fibroblasts is required for tissue repair in vivo
AuthorsLiu, S., Shi-Wen, X., Blumbach, K., Eastwood, M., Denton, C.P., Eckes, B., Krieg, T., Abraham, D.J. and Leask, A.
Abstract

In tissue repair, fibroblasts migrate into the wound to produce and remodel extracellular matrix (ECM). Integrins are believed to be crucial for tissue repair, but their tissue-specific role in this process is poorly understood. Here, we show that mice containing a fibroblast-specific deletion of integrin β1 exhibit delayed cutaneous wound closure and less granulation tissue formation, including reduced production of new ECM and reduced expression of α-smooth muscle actin (α-SMA). Integrin-β1-deficient fibroblasts showed reduced expression of type I collagen and connective tissue growth factor, and failed to differentiate into myofibroblasts as a result of reduced α-SMA stress fiber formation. Loss of integrin β1 in adult fibroblasts reduced their ability to adhere to, to spread on and to contract ECM. Within stressed collagen matrices, integrin-β1-deficient fibroblasts showed reduced activation of latent TGFβ. Addition of active TGFβ alleviated the phenotype of integrin-β1-deficient mice. Thus integrin β1 is essential for normal wound healing, where it acts, at least in part, through a TGFβ-dependent mechanism in vivo.

JournalJournal of Cell Science
Journal citation123 (21), pp. 3674-3682
ISSN0021-9533
YearNov 2010
PublisherThe Company of Biologists Ltd.
Digital Object Identifier (DOI)https://doi.org/10.1242/jcs.070672
Publication dates
PublishedNov 2010

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