Field testing for toxic algae with a microarray: initial results from the MIDTAL project

McCoy, G.R., Raine, R., Medlin, L., Chen, J., Kooistra, W., Barra, L., Ruggiero, M.V., Graneli, E., Hagström, J.A., Salomon, P.S., Reguera, B., Rodríguez, F., Escalera, L., Edvardsen, B., Dittami, S.M., Eikrem, W., Lewis, J., Berzano, M., Elliott, C.T., Campbell, K. and Pazos, Y. 2013. Field testing for toxic algae with a microarray: initial results from the MIDTAL project. Pagou, P. and Hallegraeff, G. (ed.) 14th International Conference on Harmful Algae. Hersonissos, Crete-Greece 01 - 05 Nov 2010 International Society for the Study of Harmful Algae and Intergovernmental Oceanographic Commission of UNESCO.

TitleField testing for toxic algae with a microarray: initial results from the MIDTAL project
AuthorsMcCoy, G.R., Raine, R., Medlin, L., Chen, J., Kooistra, W., Barra, L., Ruggiero, M.V., Graneli, E., Hagström, J.A., Salomon, P.S., Reguera, B., Rodríguez, F., Escalera, L., Edvardsen, B., Dittami, S.M., Eikrem, W., Lewis, J., Berzano, M., Elliott, C.T., Campbell, K. and Pazos, Y.
EditorsPagou, P. and Hallegraeff, G.
TypeConference paper
Abstract

One of the key tasks in the project MIDTAL (MIcroarrays for the Detection of Toxic ALgae) is to demonstrate the applicability of microarrays to monitor harmful algae across a broad range of ecological niches and toxic species responsible for harmful algal events. Water samples are collected from a series of sites used in national phytoplankton and biotoxin monitoring programmes across Europe. The samples are filtered; the rRNA is extracted, labelled with a fluorescent dye and applied to a microarray chip. The signal intensity from >120 probes previously spotted on the chip is measured and analysed. Preliminary results comparing microarray signal intensities with actual field counts are presented.

Year2013
Conference14th International Conference on Harmful Algae
PublisherInternational Society for the Study of Harmful Algae and Intergovernmental Oceanographic Commission of UNESCO
Publisher's versionField testing for toxic algae with a microarray_initial results from the MIDTAL project_2013.pdf
Publication dates
Published2013
Journal citationpp. 188-190
Book titleProceedings of the 14th International Conference on Harmful Algae
ISBN9788799082735
Web address (URL) of conference proceedingshttp://www.issha.org/index.php/Welcome-to-ISSHA/Conferences/ICHA-conference-proceedings/ICHA14-Proceedings

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