| Authors | Zhang, Rui, Yang, Yuyan, He, Chao, Zhang, Xin, Torraca, Vincenzo, Wang, Shen, Liu, Nan, Yang, Jiaren, Liu, Shicheng, Yuan, Jinglei, Gou, Dongzhi, Li, Shi, Dong, Xueying, Xie, Yufei, He, Junling, Bai, Hua, Hu, Mengyu, Liao, Zhiquan, Huang, Yuan, Lyu, Hao, Xiao, Shuai, Guo, Dong, Ali, Declan William, Michalak, M., Ma, C., Chen, X., Tang, J. and Zhou, C. |
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| Abstract | Autophagy serves as a pro-survival mechanism for a cell or a whole organism to cope with nutrient stress. Our understanding of the molecular regulation of this fusion event remains incomplete. Here, we identified RUNDC1 as a novel ATG14-interacting protein, which is highly conserved across vertebrates, including zebrafish and humans. By gain and loss of function studies, we demonstrate that RUNDC1 negatively modulates autophagy by blocking fusion between autophagosomes and lysosomes via inhibiting the assembly of the STX17-SNAP29-VAMP8 complex both in human cells and the zebrafish model. Moreover, RUNDC1 clasps the ATG14-STX17-SNAP29 complex via stimulating ATG14 homo-oligomerization to inhibit ATG14 dissociation. This also prevents VAMP8 from binding to STX17-SNAP29. We further identified that phosphorylation of RUNDC1 Ser is crucial to inhibit the assembly of the STX17-SNAP29-VAMP8 complex via promoting ATG14 homo-oligomerization. In line with our findings, RunDC1 is crucial for zebrafish in their response to nutrient-deficient conditions. Taken together, our findings demonstrate that RUNDC1 is a negative regulator of autophagy that restricts autophagosome fusion with lysosomes by clasping the ATG14-STX17-SNAP29 complex to hinder VAMP8 binding. [Abstract copyright: © 2023. The Author(s), under exclusive licence to ADMC Associazione Differenziamento e Morte Cellulare.] |
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