Macrophage-pathogen interactions in infectious diseases: new therapeutic insights from the zebrafish host model

RUNDC1 inhibits autolysosome formation and survival of zebrafish via clasping ATG14-STX17-SNAP29 complex
Zhang, Rui, Yang, Yuyan, He, Chao, Zhang, Xin, Torraca, Vincenzo, Wang, Shen, Liu, Nan, Yang, Jiaren, Liu, Shicheng, Yuan, Jinglei, Gou, Dongzhi, Li, Shi, Dong, Xueying, Xie, Yufei, He, Junling, Bai, Hua, Hu, Mengyu, Liao, Zhiquan, Huang, Yuan, Lyu, Hao, Xiao, Shuai, Guo, Dong, Ali, Declan William, Michalak, M., Ma, C., Chen, X., Tang, J. and Zhou, C. 2023. RUNDC1 inhibits autolysosome formation and survival of zebrafish via clasping ATG14-STX17-SNAP29 complex. Cell Death and Differentiation. 30, p. 2231–2248. https://doi.org/10.1038/s41418-023-01215-z

Shigella serotypes associated with carriage in humans establish persistent infection in zebrafish
Vincenzo Torraca, Dominik Brokatzky, Sydney L Miles, Charlotte E Chong, P Malaka De Silva, Stephen Baker, Claire Jenkins, Kathryn E Holt, Kate S Baker and Serge Mostowy 2023. Shigella serotypes associated with carriage in humans establish persistent infection in zebrafish. The Journal of Infectious Diseases. 228 (8), pp. 1108-1118. https://doi.org/10.1093/infdis/jiad326

Acquisition of a large virulence plasmid (pINV) promoted temperature-dependent virulence and global dispersal of O96:H19 enteroinvasive Escherichia coli
Sydney L. Miles, Vincenzo Torraca, Zoe A. Dyson, Ana Teresa López-Jiménez, Ebenezer Foster-Nyarko, Damián Lobato-Márquez, Claire Jenkins, Kathryn E. Holt and Serge Mostowy 2023. Acquisition of a large virulence plasmid (pINV) promoted temperature-dependent virulence and global dispersal of O96:H19 enteroinvasive Escherichia coli. mBio. 14 (4). https://doi.org/10.1128/mbio.00882-23

Zebrafish null mutants of Sept6 and Sept15 are viable but susceptible to Shigella infection
Torraca, Vincenzo, Bielecka, Magdalena K., Gomes, Margarida C., Brokatzky, Dominik, Busch‐Nentwich, Elisabeth M. and Mostowy, S. 2023. Zebrafish null mutants of Sept6 and Sept15 are viable but susceptible to Shigella infection. Cytoskeleton. 80 (7-8), pp. 266-274. https://doi.org/10.1002/cm.21750

P1 Bacteriophage-Enabled Delivery of CRISPR-Cas9 Antimicrobial Activity Against Shigella flexneri
Yang W. Huan, Vincenzo Torraca, Russell Brown, Jidapha Fa-arun, Sydney L. Miles, Diego A. Oyarzún, Serge Mostowy and Baojun Wang 2023. P1 Bacteriophage-Enabled Delivery of CRISPR-Cas9 Antimicrobial Activity Against Shigella flexneri. ACS Synthetic Biology. 12 (3), pp. 709-721. https://doi.org/10.1021/acssynbio.2c00465

Septins promote caspase activity and coordinate mitochondrial apoptosis
Hoan Van Ngo, Stevens Robertin, Dominik Brokatzky, Magdalena K. Bielecka, Damián Lobato‐Márquez, Vincenzo Torraca and Serge Mostowy 2023. Septins promote caspase activity and coordinate mitochondrial apoptosis. Cytoskeleton. 80 (7/8), pp. 254-265. https://doi.org/10.1002/cm.21696

Editorial: Zebrafish Models for Human Disease Studies
Zang, L., Torraca, V., Shimada, Y. and Nishimura, N. 2022. Editorial: Zebrafish Models for Human Disease Studies. Frontiers in Cell and Developmental Biology. 10 861941. https://doi.org/10.3389/fcell.2022.861941

Editorial: Nucleic Acid-Associated Inflammation
Laguette, N., Langevin, C., Olagnier, D., Torraca, V., Vanpouille-Box, C. and Verrier, E.R. 2021. Editorial: Nucleic Acid-Associated Inflammation. Frontiers in Immunology. 12. https://doi.org/10.3389/fimmu.2021.791580

Disruption of Cxcr3 chemotactic signaling alters lysosomal function and renders macrophages more microbicidal
Frida Sommer, Vincenzo Torraca, Yufei Xie, Aliede E. in ‘t Veld, Joost Willemse and Annemarie H. Meijer 2021. Disruption of Cxcr3 chemotactic signaling alters lysosomal function and renders macrophages more microbicidal. Cell Reports. 35 (2) 109000. https://doi.org/10.1016/j.celrep.2021.109000

Deficiency in the autophagy modulator Dram1 exacerbates pyroptotic cell death of Mycobacteria-infected macrophages
Rui Zhang, Monica Varela, Gabriel Forn-Cuní, Vincenzo Torraca, Michiel van der Vaart and Annemarie H. Meijer 2020. Deficiency in the autophagy modulator Dram1 exacerbates pyroptotic cell death of Mycobacteria-infected macrophages. Cell Death and Disease. 11 277. https://doi.org/10.1038/s41419-020-2477-1

Shigella sonnei
Vincenzo Torraca, Kathryn Holt and Serge Mostowy 2020. Shigella sonnei. Trends in Microbiology. 28 (8), pp. P696-697. https://doi.org/10.1016/j.tim.2020.02.011

Frontline Science: Antagonism between regular and atypical Cxcr3 receptors regulates macrophage migration during infection and injury in zebrafish
Sommer, Frida, Torraca, V., Kamel, Sarah M., Lombardi, Amber and Meijer, Annemarie H. 2020. Frontline Science: Antagonism between regular and atypical Cxcr3 receptors regulates macrophage migration during infection and injury in zebrafish. Journal of Leukocyte Biology. 107 (2), pp. 185-203. https://doi.org/10.1002/jlb.2hi0119-006r

Chemokine Receptors and Phagocyte Biology in Zebrafish
Sommer, Frida, Torraca, V. and Meijer, Annemarie H. 2020. Chemokine Receptors and Phagocyte Biology in Zebrafish. Frontiers in Immunology. 11 325. https://doi.org/10.3389/fimmu.2020.00325

Supplementary RNAseq dataset files
Frida Sommer, Torraca, V. and Annemarie H. Meijer 2020. Supplementary RNAseq dataset files. Zenodo. https://doi.org/10.5281/zenodo.3833847

Analysis tools to quantify dissemination of pathology in zebrafish larvae
David R. Stirling, Oniz Suleyman, Eliza Gil, Philip M. Elks, Vincenzo Torraca, Mahdad Noursadeghi and Gillian S. Tomlinson 2020. Analysis tools to quantify dissemination of pathology in zebrafish larvae. Scientific Reports. 10 3149. https://doi.org/10.1038/s41598-020-59932-1

In vivo biomolecular imaging of zebrafish embryos using confocal Raman spectroscopy
Håkon Høgset, Conor C. Horgan, James P. K. Armstrong, Mads S. Bergholt, Vincenzo Torraca, Qu Chen, Timothy J. Keane, Laurence Bugeon, Margaret J. Dallman, Serge Mostowy and Molly M. Stevens 2020. In vivo biomolecular imaging of zebrafish embryos using confocal Raman spectroscopy. Nature Communications . 11 6172. https://doi.org/10.1038/s41467-020-19827-1

Shigella sonnei O-Antigen Inhibits Internalization, Vacuole Escape, and Inflammasome Activation
Watson, Jayne L., Sanchez-Garrido, Julia, Goddard, Philippa J., Torraca, V., Mostowy, Serge, Shenoy, Avinash R. and Clements, Abigail 2019. Shigella sonnei O-Antigen Inhibits Internalization, Vacuole Escape, and Inflammasome Activation. mBio. 10 (6) e02654-19. https://doi.org/10.1128/mbio.02654-19

Shigella sonnei infection of zebrafish reveals that O-antigen mediates neutrophil tolerance and dysentery incidence
Vincenzo Torraca, Myrsini Kaforou, Jayne Watson, Gina M. Duggan, Hazel Guerrero-Gutierrez, Sina Krokowski, Michael Hollinshead, Thomas B. Clarke, Rafal J. Mostowy, Gillian S. Tomlinson, Vanessa Sancho-Shimizu, Abigail Clements and Serge Mostowy 2019. Shigella sonnei infection of zebrafish reveals that O-antigen mediates neutrophil tolerance and dysentery incidence. PLOS Pathogens. 15 (12) e1008006. https://doi.org/10.1371/journal.ppat.1008006

RNAseq Profiling of Leukocyte Populations in Zebrafish Larvae Reveals a cxcl11 Chemokine Gene as a Marker of Macrophage Polarization During Mycobacterial Infection
Rougeot, Julien, Torraca, V., Zakrzewska, Ania, Kanwal, Zakia, Jansen, Hans J., Sommer, Frida, Spaink, Herman P. and Meijer, Annemarie H. 2019. RNAseq Profiling of Leukocyte Populations in Zebrafish Larvae Reveals a cxcl11 Chemokine Gene as a Marker of Macrophage Polarization During Mycobacterial Infection. Frontiers in Immunology. 10 832. https://doi.org/10.3389/fimmu.2019.00832

CXCR4 signaling regulates metastatic onset by controlling neutrophil motility and response to malignant cells
Annemarie Meijer and Torraca, V. 2019. CXCR4 signaling regulates metastatic onset by controlling neutrophil motility and response to malignant cells. Scientific Reports. 9 2399. https://doi.org/10.1038/s41598-019-38643-2

Macrophages target Salmonella by Lc3-associated phagocytosis in a systemic infection model
Masud, Samrah, Prajsnar, Tomasz K., Torraca, V., Lamers, Gerda E. M., Benning, Marianne, Van der Vaart, Michiel and Meijer, Annemarie H. 2019. Macrophages target Salmonella by Lc3-associated phagocytosis in a systemic infection model. Autophagy. 15 (5), pp. 796-812. https://doi.org/10.1080/15548627.2019.1569297

Meeting report: Zebrafish Infection and Immunity 2019
Torraca, V., Gomes, Margarida C., Sarris, Milka and Mostowy, Serge 2019. Meeting report: Zebrafish Infection and Immunity 2019. Lab Animal. 48, pp. 284-287. https://doi.org/10.1038/s41684-019-0400-0

Zebrafish Infection: From Pathogenesis to Cell Biology
Vincenzo Torraca and Serge Mostowy 2018. Zebrafish Infection: From Pathogenesis to Cell Biology. Trends in Cell Biology. 28 (2), pp. P143-156. https://doi.org/10.1016/j.tcb.2017.10.002

Shigella-Induced Emergency Granulopoiesis Protects Zebrafish Larvae from Secondary Infection
Willis, Alexandra R., Torraca, V., Gomes, Margarida C., Shelley, Jennifer, Mazon-Moya, Maria, Filloux, Alain, Lo Celso, Cristina and Mostowy, Serge 2018. Shigella-Induced Emergency Granulopoiesis Protects Zebrafish Larvae from Secondary Infection. mBio. 9 (3) e00933-18. https://doi.org/10.1128/mbio.00933-18

Septins restrict inflammation and protect zebrafish larvae from Shigella infection
Maria J. Mazon-Moya, Alexandra R. Willis, Vincenzo Torraca, Laurent Boucontet, Avinash R. Shenoy, Emma Colucci-Guyon and Serge Mostowy 2017. Septins restrict inflammation and protect zebrafish larvae from Shigella infection. PLOS Pathogens. 13 (6) e1006467. https://doi.org/10.1371/journal.ppat.1006467

The inflammatory chemokine Cxcl18b exerts neutrophil-specific chemotaxis via the promiscuous chemokine receptor Cxcr2 in zebrafish
Torraca, V., Otto, Natasja A., Tavakoli-Tameh, Aidin and Meijer, Annemarie H. 2017. The inflammatory chemokine Cxcl18b exerts neutrophil-specific chemotaxis via the promiscuous chemokine receptor Cxcr2 in zebrafish. Developmental & Comparative Immunology. 67, pp. 57-65. https://doi.org/10.1016/j.dci.2016.10.014

The chemokine receptor CXCR4 promotes granuloma formation by sustaining a mycobacteria-induced angiogenesis programme
Annemarie Meijer and Torraca, V. 2017. The chemokine receptor CXCR4 promotes granuloma formation by sustaining a mycobacteria-induced angiogenesis programme. Scientific Reports. 7 45061. https://doi.org/10.1038/srep45061

Functional analysis reveals no transcriptional role for the glucocorticoid receptor beta-isoform in zebrafish
Chatzopoulou, Antonia, Schoonheim, Peter J., Torraca, V., Meijer, Annemarie H., Spaink, Herman P. and Schaaf, Marcel J.M. 2017. Functional analysis reveals no transcriptional role for the glucocorticoid receptor beta-isoform in zebrafish. Molecular and Cellular Endocrinology. 447, pp. 61-70. https://doi.org/10.1016/j.mce.2017.02.036

Modeling Infectious Diseases in the Context of a Developing Immune System
Masud, Samrah, Torraca, V. and Meijer, Annemarie H. 2017. Modeling Infectious Diseases in the Context of a Developing Immune System. Current Topics in Developmental Biology. 124, pp. 277-329. https://doi.org/10.1016/bs.ctdb.2016.10.006

Septins and Bacterial Infection
Torraca, V. and Mostowy, Serge 2016. Septins and Bacterial Infection. Frontiers in Cell and Developmental Biology. https://doi.org/10.3389/fcell.2016.00127

The CXCR3-CXCL11 signaling axis mediates macrophage recruitment and dissemination of mycobacterial infection
Torraca, V., Cui, Chao, Boland, Ralf, Bebelman, Jan-Paul, van der Sar, Astrid M., Smit, Martine J., Siderius, Marco, Spaink, Herman P. and Meijer, Annemarie H. 2015. The CXCR3-CXCL11 signaling axis mediates macrophage recruitment and dissemination of mycobacterial infection. Disease Models and Mechanisms. 8 (3), pp. 253-269. https://doi.org/10.1242/dmm.017756

Robotic injection of zebrafish embryos for high-throughput screening in disease models
Spaink, Herman P., Cui, Chao, Wiweger, Malgorzata I., Jansen, Hans J., Veneman, Wouter J., Marin-Juez, Ruben, de Sonneville, Jan, Ordas, Anita, Torraca, V., van der Ent, Wietske, Leenders, William P., Meijer, Annemarie H., Snaar-Jagalska, B. Ewa and Dirks, Ron P. 2013. Robotic injection of zebrafish embryos for high-throughput screening in disease models. Methods. 62 (3), pp. 246-254. https://doi.org/10.1016/j.ymeth.2013.06.002

Ultra-small graphene oxide functionalized with polyethylenimine (PEI) for very efficient gene delivery in cell and zebrafish embryos
Zhou, Xiang, Laroche, Fabrice, Lamers, Gerda E. M., Torraca, V., Voskamp, Patrick, Lu, Tao, Chu, Fuqiang, Spaink, Herman P., Abrahams, Jan Pieter and Liu, Zunfeng 2012. Ultra-small graphene oxide functionalized with polyethylenimine (PEI) for very efficient gene delivery in cell and zebrafish embryos. Nano Research. 5, pp. 703-709. https://doi.org/10.1007/s12274-012-0254-x