Total collagen content and distribution is increased in human colon during advancing age : WestminsterResearch

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Title	Total collagen content and distribution is increased in human colon during advancing age
Type	Journal article
Authors	Nicholas Baidoo, Ellie Crawley, Charles H. Knowles, Gareth J. Sanger and Abi Belai
Abstract	Background The effect of ageing on total collagen content of human colon has been poorly investigated. The aim of this study was to determine if ageing altered total collagen content and distribution in the human colon. Methods Macroscopically normal ascending colon was obtained at surgery from cancer patients (n = 31) without diagnosis of diverticular disease or inflammatory bowel disease. Masson’s trichrome and Picrosirius red stains were employed to identify the total collagen content and distribution within the sublayers of the colonic wall for adult (22–60 years; 6 males, 6 females) and elderly (70 – 91years; 6 males, 4 female) patients. A hydroxyproline assay evaluated the total collagen concentration for adult (30–64 years; 9 male, 6 female) and elderly (66–91 years; 8 male, 8 female) patients. Key results Histological studies showed that the percentage mean intensity of total collagen staining in the mucosa, submucosa and muscularis externa was, respectively, 14(1.9) %, 74(3.2) % and 12(1.5) % in the adult ascending colon. Compared with the adults, the total collagen fibres content was increased in the submucosa (mean intensity; 163.1 ± 11.1 vs. 124.5 ± 7.8; P < 0.05) and muscularis externa (42.5 ± 8.0 vs. 20.6 ± 2.8; P < 0.01) of the elderly patients. There was no change in collagen content of the mucosa. The total collagen concentration was increased in the elderly by 16%. Sex-related differences were not found, and data were combined for analysis. Conclusions Greater total collagen content was found in the submucosa and muscularis externa of the elderly human male and female colon. These changes may contribute to a possible loss of function with ageing.
Article number	e0269689
Journal	PLoS ONE
Journal citation	17 (6)
ISSN	1932-6203
Year	2022
Publisher	Public Library of Science
Publisher's version	journal.pone.0269689.pdf License CC BY 4.0 File Access Level Open (open metadata and files)
Digital Object Identifier (DOI)	https://doi.org/10.1371/journal.pone.0269689
Web address (URL)	https://doi.org/10.1371/journal.pone.0269689
Published	17 Jun 2022
Editors	Amitava Mukherjee

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Total collagen content and distribution is increased in human colon during advancing age

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