The vulnerability of the human taenia coli to alterations in total collagen within the colon of the elderly

Baidoo, N., Sanger, G.J. and Belai, A. 2022. The vulnerability of the human taenia coli to alterations in total collagen within the colon of the elderly. Acta Histochemica. 124 (8) 151958. https://doi.org/10.1016/j.acthis.2022.151958

TitleThe vulnerability of the human taenia coli to alterations in total collagen within the colon of the elderly
TypeJournal article
AuthorsBaidoo, N., Sanger, G.J. and Belai, A.
Abstract

The structure of the colonic wall relies on collagen, distributed within the submucosa and the muscularis externa. A recent analysis of total collagen in human ascending colon (AC) suggests that the muscularis externa is more susceptible to age-related increases in collagen among the elderly. However, it is not clear if this change also occurs in the descending colon (DC) or if the circular and longitudinal muscle layers are similarly affected in either region of colon. The aim of this study is to determine the total collagen content in the DC and its distribution between the circular muscle (CM) and taenia coli (TC) of the AC and DC of adults and compare the same with tissue from the elderly. Masson’s trichrome and Picrosirius red were used to assess total collagen content in the AC and DC; aged 22 – 91 years. Macroscopically normal AC from 22 patients (adults: 22–60 years; 6 male, 6 female; elderly: 70 – 91 years; 6 male, 4 female) and DC from 23 patients (adults: 23–63 years; 6 male, 7 female; elderly: 66 – 88 years; 6 male, 4 female) were obtained following surgery for non-obstructed bowel cancer. The total hydroxyproline content in DC samples was also evaluated. In the DC, tinctorial staining demonstrated an increased occurrence of total collagen fibres in the submucosa of the elderly (159.8 ± 9.6 in elderly vs. 126.9 ± 6.1 in the adults; p 0.05) and in the muscularis externa (respectively 37.4 ± 4.1 vs. 18.8 ± 2.4; p 0.01). In the adult AC and DC, there were no statistically significant differences in the amount of collagen within the CM and TC. In the elderly, the total collagen fibres within the TC was greater in the AC (mean grey intensity: 63.4 ± 3.9% in the elderly vs. 36.6 ± 1.6% in adults; p 0.05) and DC (mean grey intensity: respectively, 59.82 ± 2.4 vs. 40.2 ± 0.9%; p 0.05). In both AC and DC of the elderly samples, several thickened collagen fibrils were microscopically identified within the TC infiltrating to the myenteric plexus. In the TC of the elderly AC, the total collagen fibres were increased by approximately 4% compared to that of the DC. The total collagen concentration in the elderly DC assessed by hydroxyproline assay was increased by approximately 15% compared to the adult. Sex related differences were not found when data combined. We concluded that the total collagen content in the muscularis externa particularly of the TC of human colon increases with age. The subtle change in collagen distribution with age between AC and DC may differentially affect the tensile strength of the colon.

Article number151958
JournalActa Histochemica
Journal citation124 (8)
ISSN1618-0372
0065-1281
Year2022
PublisherElsevier
Publisher's version
License
CC BY-NC-ND 4.0
File Access Level
Open (open metadata and files)
Digital Object Identifier (DOI)https://doi.org/10.1016/j.acthis.2022.151958
Publication dates
Published06 Oct 2022

Related outputs

The impact of long‐course chemoradiotherapy on the myenteric plexus, neuromuscular functions and responses to prokinetic drugs in the human rectum
Kung, V., Broad, J., Makwana, R., Palmer, A., Baidoo, N., Epton, Sarah, Elahi, S., Chin‐Aleong, Joanne, Thaha, M., Knowles, C. and Sanger, G. 2024. The impact of long‐course chemoradiotherapy on the myenteric plexus, neuromuscular functions and responses to prokinetic drugs in the human rectum. United European Gastroenterology Journal. https://doi.org/10.1002/ueg2.12653

Age-related decline in goblet cell numbers and mucin content of the human colon: Implications for lower bowel functions in the elderly
Baidoo, N. and Sanger, G. 2024. Age-related decline in goblet cell numbers and mucin content of the human colon: Implications for lower bowel functions in the elderly. Experimental and Molecular Pathology. 139 104923. https://doi.org/10.1016/j.yexmp.2024.104923

The human colon: Evidence for degenerative changes during aging and the physiological consequences
Baidoo, N. and Sanger, G.J. 2024. The human colon: Evidence for degenerative changes during aging and the physiological consequences. Neurogastroenterology & Motility. e14848. Advanced online publication. https://doi.org/10.1111/nmo.14848

Preprint: The Distribution of Calretinin Immunoreactive Neurons in Ageing Human Colon is Region and Sublayer Specific
Baidoo, N., Sanger, G.J. and Belai, A. 2023. Preprint: The Distribution of Calretinin Immunoreactive Neurons in Ageing Human Colon is Region and Sublayer Specific. SSRN. https://doi.org/10.2139/ssrn.4432429

The effect of ageing on the innervation and total collagen content of human colon
Nicholas Baidoo 2023. The effect of ageing on the innervation and total collagen content of human colon. PhD thesis University of Roehampton School of Life and Health Sciences https://doi.org/10.13140/rg.2.2.31758.74560

Effect of old age on the subpopulations of enteric glial cells in human descending colon
Baidoo, N. 2023. Effect of old age on the subpopulations of enteric glial cells in human descending colon. 2nd London Glial Symposium. The Francis Crick Institute, London 31 Oct 2023

The Distribution of Calretinin Immunoreactive Neurons in Ageing Human Colon is Region and Sublayer Specific
Baidoo, N., Sanger, G.J. and Belai, A. 2023. The Distribution of Calretinin Immunoreactive Neurons in Ageing Human Colon is Region and Sublayer Specific. 30th International Student Congress of (bio)Medical Sciences. University Medical Center Groningen, Groningen, The Netherlands 05 - 08 Jun 2023 https://doi.org/10.2139/ssrn.4432429

Histochemical and biochemical analysis of collagen content in formalin-fixed, paraffin embedded colonic samples
Nicholas Baidoo, Gareth J. Sanger and Abi Belai 2023. Histochemical and biochemical analysis of collagen content in formalin-fixed, paraffin embedded colonic samples. MethodsX. 11 102416. https://doi.org/10.1016/j.mex.2023.102416

Effect of old age on the subpopulations of enteric glial cells in human descending colon
Nicholas Baidoo, Gareth J. Sanger and Abi Belai 2023. Effect of old age on the subpopulations of enteric glial cells in human descending colon. GLIA. 71 (2), pp. 305-316. https://doi.org/10.1002/glia.24272

Effect of old age on the subpopulations of enteric glial cells in human descending colon
Baidoo, N. 2022. Effect of old age on the subpopulations of enteric glial cells in human descending colon. 30th United European Gastroenterology Week 2022. Messe Wien Exhibition & Congress Center, Vienna, Austria 08 - 11 Oct 2022 https://doi.org/10.1002/ueg2.12294

Total collagen content and distribution is increased in human colon during advancing age
Baidoo, N. 2022. Total collagen content and distribution is increased in human colon during advancing age. 29th International Student Congress Of (bio)Medical Sciences. University Medical Center Groningen, Groningen, The Netherlands 08 - 10 Jun 2022

Total collagen content and distribution is increased in human colon during advancing age
Nicholas Baidoo, Ellie Crawley, Charles H. Knowles, Gareth J. Sanger and Abi Belai 2022. Total collagen content and distribution is increased in human colon during advancing age. PLoS ONE. 17 (6) e0269689. https://doi.org/10.1371/journal.pone.0269689

Permalink - https://westminsterresearch.westminster.ac.uk/item/w85q9/the-vulnerability-of-the-human-taenia-coli-to-alterations-in-total-collagen-within-the-colon-of-the-elderly


Share this

Usage statistics

28 total views
8 total downloads
These values cover views and downloads from WestminsterResearch and are for the period from September 2nd 2018, when this repository was created.