Estimation of total phenol and in vitro antioxidant activity of Albizia procera leaves

Khatoon, M., Islam, E., Islam, R., Rahman, A.A., Alam, A.H.M.K., Khondkar, P., Rashid, M. and Parvin, S. 2013. Estimation of total phenol and in vitro antioxidant activity of Albizia procera leaves. BMC Research Notes. 6. https://doi.org/10.1186/1756-0500-6-121

TitleEstimation of total phenol and in vitro antioxidant activity of Albizia procera leaves
TypeJournal article
AuthorsKhatoon, M., Islam, E., Islam, R., Rahman, A.A., Alam, A.H.M.K., Khondkar, P., Rashid, M. and Parvin, S.
Abstract

Background
Research on natural products has gained a wide popularity due to the potential of discovering active compounds. The antioxidant properties contained in plants have been proposed as one of the mechanisms for the observed beneficial effect. Therefore, the present study investigated the antioxidant activity and total phenolic contents of various solvent extracts of Albizia procera leaves.

Methods
Antioxidant activity of the methanol extract and its derived fractions petroleum ether (APP), carbon tetrachloride (APC), dichloromethane (APD), ethyl acetate (APE), and residual aqueous fraction (APA) of the leaves of Albizia procera was performed by in vitro chemical analyses. Total phenolic content of the APM and other five fractions were also determined. APM and its derived fractions were also subjected to preliminary phytochemical screening test for various constituents.

Results
Phytochemical screening revealed the presence of saponins, steroids, tannins, glycosides and flavonoids in the extracts. Amongst the extracts, APE showed the highest total phenolic content (449.18 ± 18.41mg of gallic acid equivalent/g of extract). In DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging test, the IC50 value of APM, APP, APC, APD, APE and APA was 43.43, 63.60, 166.18, 41.15, 11.79, and 63.06 μg/mL, respectively. Therefore, among the APM and its derived fractions, APE showed the highest antioxidant activity which is comparable to that of standard ascorbic acid (AA) (IC50 10.12 μg/mL). The total antioxidant capacity was found to be varied in different fractions. The reducing activity on ferrous ion was ranked as APE > APD > APM > APA > APC.

Conclusion
The above evidences suggest that APE of A. procera leaf is a potential source of natural antioxidant and can be used to prevent diseases associated with free radicals.

JournalBMC Research Notes
Journal citation6
ISSN1756-0500
Year2013
PublisherBioMed Central
Digital Object Identifier (DOI)https://doi.org/10.1186/1756-0500-6-121
Publication dates
Published2013

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