Ethanolic extract of the Goldenseal, Hydrastis canadensis, has demonstrable chemopreventive effects on HeLa cells in vitro: Drug--DNA interaction with calf thymus DNA as target

Saha, S., Sikdar, S., Mukherjee, A., Bhadra, K., Boujedaini, N. and Khuda-Bukhsh, A.R. 2013. Ethanolic extract of the Goldenseal, Hydrastis canadensis, has demonstrable chemopreventive effects on HeLa cells in vitro: Drug--DNA interaction with calf thymus DNA as target. Environmental Toxicology and Pharmacology. 36 (1), pp. 202-214. https://doi.org/10.1016/j.etap.2013.03.023

TitleEthanolic extract of the Goldenseal, Hydrastis canadensis, has demonstrable chemopreventive effects on HeLa cells in vitro: Drug--DNA interaction with calf thymus DNA as target
TypeJournal article
AuthorsSaha, S., Sikdar, S., Mukherjee, A., Bhadra, K., Boujedaini, N. and Khuda-Bukhsh, A.R.
Abstract

This study tested chemotherapeutic potential of Hydrastis canadensis (HC) extract in HeLa cells in vitro, with emphasis on its drug–DNA interaction and apoptosis induction ability. Nuclear uptake of HC by DAPI, Ao/Eb staining and internucleosomal DNA damage by comet assay was studied through fluorescence microscopy. Possible changes in MMP and apoptotic signalling events were critically analyzed. Cell cycle progression studied through FACS and fragmented DNA through “TUNEL” assay were critically analyzed. RT-PCR studies were conducted for analyzing Cyt-C and Bax translocation in mitochondrial and cytosolic extracts, and Caspase 3 in whole cell lysate. Role of p53-mediated regulation of NF-κβ and TNF-α was elucidated by Western blot analysis. Data of CD and Tm profile of CT-DNA were analyzed. Overall results indicated anti-cancer potential of HC through its ability to induce apoptosis, and interaction with CT-DNA that changed structural conformation of DNA, proving HC to be a promising candidate for chemoprevention.

JournalEnvironmental Toxicology and Pharmacology
Journal citation36 (1), pp. 202-214
ISSN1382-6689
1872-7077
Year2013
PublisherElsevier
Digital Object Identifier (DOI)https://doi.org/10.1016/j.etap.2013.03.023
Publication dates
PublishedJul 2013

Related outputs

First person – Santu Saha
Saha, S. 2023. First person – Santu Saha. Biology Open. 12 (5) bio059987. https://doi.org/10.1242/bio.059987

Bioengineering of a tumour-stroma 3D-tumouroid co-culture model of hypopharyngeal cancer.
Saha, S., Howarth, R., Sharma-Saha, S. and Kelly, C. 2023. Bioengineering of a tumour-stroma 3D-tumouroid co-culture model of hypopharyngeal cancer. Biology Open. 12 (5) bio059949. https://doi.org/10.1242/bio.059949

Determining the Potential of DNA Damage Response (DDR) Inhibitors in Cervical Cancer Therapy.
Saha, S., Rundle S, Kotsopoulos IC, Begbie J, Howarth R, Pappworth IY, Mukhopadhyay A, Kucukmetin A, Marchbank KJ and Curtin N 2022. Determining the Potential of DNA Damage Response (DDR) Inhibitors in Cervical Cancer Therapy. Cancers. 14 (17) 4288. https://doi.org/10.3390/cancers14174288

Survival outcomes of parotid carcinoma treated with surgery and radiotherapy or radiotherapy alone.
Iqbal, M.S., Jamil, F., Kovarik, J., Cunnell, M., Kelly, C. and Saha, S. 2022. Survival outcomes of parotid carcinoma treated with surgery and radiotherapy or radiotherapy alone. ICHNO–ECHNO 2022 Congress. Brussels, Belgium 03 - 05 Mar 2022

Molecular approaches toward targeted cancer therapy with some food plant products: On the role of antioxidants and immune microenvironment
Khuda-Bukhsh, A.R., Saha, S., Das, S. and Saha. S.S. 2021. Molecular approaches toward targeted cancer therapy with some food plant products: On the role of antioxidants and immune microenvironment. in: Preedy, V.R. and Patel, V.B. (ed.) Cancer (2nd edition) Academic Press. pp. 191-202

HCV Activates Somatic L1 Retrotransposition-A Potential Hepatocarcinogenesis Pathway.
Sudhindar, P.D., Wainwright, D., Saha, S., Howarth, R., McCain, M., Bury, Y., Saha, S.S., McPherson, S., Reeves, H., Patel, A.H., Faulkner, G.J., Lunec, J. and Shukla, R. 2021. HCV Activates Somatic L1 Retrotransposition-A Potential Hepatocarcinogenesis Pathway. Cancers. 13 (20) 5079. https://doi.org/10.3390/cancers13205079

PARP inhibitors: A new frontier in the battle against cervical cancer
Saha, S. 2021. PARP inhibitors: A new frontier in the battle against cervical cancer. SelectScience.

Introduction to Cancer Stem Cells
Saha, S., Khuda-Bukhsh, A.R., Samadder, A. and Saha, S.K. 2020. Introduction to Cancer Stem Cells. in: Pathak, S. and Banerjee, A. (ed.) Cancer Stem Cells: New Horizons in Cancer Therapies Springer.

Potential use of the PARP inhibitor rucaparib to enhance cervical cancer treatment
Saha, S., Howarth, R., Pappworth, I., Marchbank, K. and Curtin, N. 2020. Potential use of the PARP inhibitor rucaparib to enhance cervical cancer treatment. European Journal of Cancer. 138 (Supp 2), p. S37. https://doi.org/10.1016/s0959-8049(20)31169-2

SP-0517: Exploring rucaparib as chemo-radiosensitiser to increase therapeutic ratio in cervical cancer
Saha, S., Howarth, R., Dubrovska, A., Marchbank, K. and Curtin, N. 2020. SP-0517: Exploring rucaparib as chemo-radiosensitiser to increase therapeutic ratio in cervical cancer. Radiotherapy & Oncology. 152 (Supp 1), pp. S283-S284. https://doi.org/10.1016/S0167-8140(21)00539-9

Association of Hyaluronan and CD44 during development of Head and Neck Squamous Cell Carcinoma in Indian patients
Mukhopadhyay, D., Chakraborty, B., Alam, N., Biswas, J., Roy, A., Roychoudhury, S., Panda, C.K. and Saha, S. 2017. Association of Hyaluronan and CD44 during development of Head and Neck Squamous Cell Carcinoma in Indian patients. Recent Trends in Microbiology Conference. RK Mission, Kolkata, India

Molecular progression of Head and Neck Squamous Cell Carcinoma
Saha, S., Maiti, G.P., Roychoudhury, S. and Panda, C.K. 2017. Molecular progression of Head and Neck Squamous Cell Carcinoma. The Nucleus. 60, pp. 111-119. https://doi.org/10.1007/s13237-017-0212-9

Molecular Approaches Toward Targeted Cancer Therapy with Some Food Plant Products
Khuda-Bukhsh, A.R., Saha, S. and Das, S. 2014. Molecular Approaches Toward Targeted Cancer Therapy with Some Food Plant Products. in: Preedy, V. (ed.) Cancer Academic Press. pp. 77-89

Molecular approaches toward targeted cancer prevention with some food plants and their products: inflammatory and other signal pathways
Khuda-Bukhsh, A.R., Das, S. and Saha, S. 2014. Molecular approaches toward targeted cancer prevention with some food plants and their products: inflammatory and other signal pathways. Nutrition and Cancer. 66 (2), pp. 194-205. https://doi.org/10.1080/01635581.2014.864420

Berberine alters epigenetic modifications, disrupts microtubule network, and modulates HPV-18 E6--E7 oncoproteins by targeting p53 in cervical cancer cell HeLa: A mechanistic study including molecular docking
Saha, S. and Khuda-Bukhsh, A.R. 2014. Berberine alters epigenetic modifications, disrupts microtubule network, and modulates HPV-18 E6--E7 oncoproteins by targeting p53 in cervical cancer cell HeLa: A mechanistic study including molecular docking. European Journal of Pharmacology. 744, pp. 132-146. https://doi.org/10.1016/j.ejphar.2014.09.048

Water-Soluble Heteronuclear [NaCuII6] Metallomacrocyclic Sandwich Complexes: Synthesis, Structure, Properties and In Vitro Biological Studies
Patra, A., Saha, S., Sen, T.K., Carrella, L., Musie, G.T., Khuda-Bukhsh, A.R. and Bera, M. 2014. Water-Soluble Heteronuclear [NaCuII6] Metallomacrocyclic Sandwich Complexes: Synthesis, Structure, Properties and In Vitro Biological Studies. European Journal of Inorganic Chemistry. Volume 2014 (30), pp. 5217-5232. https://doi.org/10.1002/ejic.201402582

Poly (lactic-co-glycolic) acid loaded nano-insulin has greater potentials of combating arsenic induced hyperglycemia in mice: some novel findings
Asmita Samadder, A., Das, J., Das, S., De, A., Saha, S., Bhattacharyya, S.S. and Khuda-Bukhsh, A.R. 2013. Poly (lactic-co-glycolic) acid loaded nano-insulin has greater potentials of combating arsenic induced hyperglycemia in mice: some novel findings. Toxicology and Applied Pharmacology. 267 (1), pp. 57-73. https://doi.org/10.1016/j.taap.2012.12.018

Molecular approaches towards development of purified natural products and their structurally known derivatives as efficient anti-cancer drugs: current trends
Saha, S. and Khuda-Bukhsh, A.R. 2009. Molecular approaches towards development of purified natural products and their structurally known derivatives as efficient anti-cancer drugs: current trends. European Journal of Pharmacology. 714 (1-3), pp. 239-248. https://doi.org/10.1016/j.ejphar.2013.06.009

Permalink - https://westminsterresearch.westminster.ac.uk/item/x521y/ethanolic-extract-of-the-goldenseal-hydrastis-canadensis-has-demonstrable-chemopreventive-effects-on-hela-cells-in-vitro-drug-dna-interaction-with-calf-thymus-dna-as-target


Share this

Usage statistics

21 total views
0 total downloads
These values cover views and downloads from WestminsterResearch and are for the period from September 2nd 2018, when this repository was created.