Schaeffer, M., Hodson, D.J., Meunier, A.C., Lafont, C., Birkenstock, J., Carmignac, D., Murray, J.F., Gavois, E., Robinson, I.C., Le Tissier, P. and Mollard, P. 2011. Influence of estrogens on GH-cell network dynamics in females: a live in situ imaging approach. Endocrinology. 152 (12), pp. 4789-4799. https://doi.org/10.1210/en.2011-1430
| Title | Influence of estrogens on GH-cell network dynamics in females: a live in situ imaging approach |
|---|---|
| Authors | Schaeffer, M., Hodson, D.J., Meunier, A.C., Lafont, C., Birkenstock, J., Carmignac, D., Murray, J.F., Gavois, E., Robinson, I.C., Le Tissier, P. and Mollard, P. |
| Abstract | The secretion of endocrine hormones from pituitary cells finely regulates a multitude of homeostatic processes. To dynamically adapt to changing physiological status and environmental stimuli, the pituitary gland must undergo marked structural and functional plasticity. Endocrine cell plasticity is thought to primarily rely on variations in cell proliferation and size. However, cell motility, a process commonly observed in a variety of tissues during development, may represent an additional mechanism to promote plasticity within the adult pituitary gland. To investigate this, we used multiphoton time-lapse imaging methods, GH-enhanced green fluorescent protein transgenic mice and sexual dimorphism of the GH axis as a model of divergent tissue demand. Using these methods to acutely (12 h) track cell dynamics, we report that ovariectomy induces a dramatic and dynamic increase in cell motility, which is associated with gross GH-cell network remodeling. These changes can be prevented by estradiol supplementation and are associated with enhanced network connectivity as evidenced by increased coordinated GH-cell activity during multicellular calcium recordings. Furthermore, cell motility appears to be sex-specific, because reciprocal alterations are not detected in males after castration. Therefore, GH-cell motility appears to play an important role in the structural and functional pituitary plasticity, which is evoked in response to changing estradiol concentrations in the female. |
| Journal | Endocrinology |
| Journal citation | 152 (12), pp. 4789-4799 |
| ISSN | 0013-7227 |
| Year | 2011 |
| Publisher | Endocrine Society |
| Digital Object Identifier (DOI) | https://doi.org/10.1210/en.2011-1430 |
| Publication dates | |
| Published | 2011 |
The role of pituitary melanocortin receptor 3 (MC3) in regulation of hormone output
Murray, J.F., Berruien, N., Dowejko, M.M., Santiago Andres, Y, Laiho, L, Andoniadou, C, Fiordelisio, T, Le Tissier, P. and Smith, C.L. 2022. The role of pituitary melanocortin receptor 3 (MC3) in regulation of hormone output. The International Congress of Neuroendocrinology. SEC, Glasgow, Scotland, UK 07 - 10 Aug 2022
Melanocortin‐3 receptor responseis influenced by the melanocortin receptoraccessory protein‐1
Berruien, N., Le Tissier, P., Murray, J.F. and Smith, C.L. 2018. Melanocortin‐3 receptor responseis influenced by the melanocortin receptoraccessory protein‐1. Pharmacology 2018. Queen Elizabeth II conference centre London 18 - 20 Dec 2018 Wiley-Blackwell. https://doi.org/10.1111/bph.14681
Analysis of the melanocortin system in the regulation of pituitary gland function in mice
Le Tissier, P., Smith, C.L., Nuri A. Berruien, N. and Murray, J.F. 2018. Analysis of the melanocortin system in the regulation of pituitary gland function in mice. International Congress of Neuroendocinology. Toronto, Canada 15 - 18 Jul 2018
Melanin-concentrating hormone in peripheral circulation in the human
Naufahu, J., Alzaid, F., Fiuza Brito, M., Doslikova, B., Valencia, T. and Murray, J.F. 2017. Melanin-concentrating hormone in peripheral circulation in the human. Journal of Endocrinology. 232, pp. 513-523. https://doi.org/10.1530/JOE-16-0240
Effect of age and pregnancy on the expression of melanocortin receptors 4 and 5 in the mouse female reproductive axis
Smith, C.L., Nuri A. Berruien, N., Murray, J.F. and Le Tissier, P.R. 2016. Effect of age and pregnancy on the expression of melanocortin receptors 4 and 5 in the mouse female reproductive axis. BPS Pharmacology 2016. London, UK 12 - 14 Dec 2016 British Pharmacological Society.
Anatomy, embryology, and cell families
Locke, I.C. and Murray, J.F. 2014. Anatomy, embryology, and cell families. in: Orchard, G. and Nation, B. (ed.) Cell Structure & Function Oxford, UK Oxford University Press. pp. 82-110
The melanocortin system in the male reproductive axis
Dowejko, M.M., Smith, C.L., Getting, S.J., Le Tissier, P. and Murray, J.F. 2014. The melanocortin system in the male reproductive axis. Endocrine Abstracts. 34, p. P338. https://doi.org/10.1530/endoabs.34.P338
Melanocortin receptor expression in the hypothalamo-pituitary-gonadal system of WT and MC3 null mice
Dowejko, M.M., Smith, C.L., Getting, S.J., Le Tissier, P. and Murray, J.F. 2014. Melanocortin receptor expression in the hypothalamo-pituitary-gonadal system of WT and MC3 null mice. International Congress of Neuroendocrinology 2014. Sydney 17-20 August 2014
Melanocortin 3 receptors (MC3) exert anti-inflammatory effects in mouse testicular inflammation by reducing nitric oxide (NO) release from Leydig cells
Dowejko, M.M., Smith, C.L., Getting, S.J., Le Tissier, P. and Murray, J.F. 2014. Melanocortin 3 receptors (MC3) exert anti-inflammatory effects in mouse testicular inflammation by reducing nitric oxide (NO) release from Leydig cells. Society for Reproduction and Fertility Conference. EICC2, Edinburgh 1-2 September 2014
The roles of melanin-concentrating hormone in energy balance and reproductive function: are they connected?
Naufahu, J., Cunliffe, A. and Murray, J.F. 2013. The roles of melanin-concentrating hormone in energy balance and reproductive function: are they connected? Reproduction. 146, pp. R141-R150. https://doi.org/10.1530/REP-12-0385
Melanocortin 3 receptor (MC3) activation effects on testicular testosterone and nitrite production in vitro
Dowejko, M.M., Smith, C.L., Getting, S.J., Le Tissier, P. and Murray, J.F. 2013. Melanocortin 3 receptor (MC3) activation effects on testicular testosterone and nitrite production in vitro. Society for Reproduction and Fertility (SRF) Annual Conference. Cambridge 11th July 2013
Expression of melanocortin 3 receptors (MC3) in the reproductive axes of both male and female mice
Dowejko, M.M., Smith, C.L., Getting, S.J., Le Tissier, P. and Murray, J.F. 2012. Expression of melanocortin 3 receptors (MC3) in the reproductive axes of both male and female mice. Society for Reproduction and Fertility. Edinburgh 9th - 11th July 2012
Immunohistochemical assessment of intrinsic and extrinsic markers of hypoxia in reproductive tissue: differential expression of HIF1α and HIF2α in rat oviduct and endometrium
Gillies, R.M., Robinson, S.P., McPhail, L.D., Carter, N.D. and Murray, J.F. 2011. Immunohistochemical assessment of intrinsic and extrinsic markers of hypoxia in reproductive tissue: differential expression of HIF1α and HIF2α in rat oviduct and endometrium. Journal of Molecular Histology. 42 (4), pp. 341-354. https://doi.org/10.1007/s10735-011-9338-2
Synchronization of the sheep oestrous cycle
Murray, J.F. 2011. Synchronization of the sheep oestrous cycle. Animal Technology and Welfare. 10, pp. 205-208.
Novel ontogenetic patterns of sexual differentiation in arcuate nucleus GHRH neurons revealed in GHRH-enhanced green fluorescent protein transgenic mice
McArthur, S., Robinson, I.C. and Gillies, G.E. 2011. Novel ontogenetic patterns of sexual differentiation in arcuate nucleus GHRH neurons revealed in GHRH-enhanced green fluorescent protein transgenic mice. Endocrinology. 152 (2), pp. 607-617. https://doi.org/10.1210/en.2010-0798
Total pituitary hormone content in wild type and MC3 null male and female mice
Dowejko, M.M., Smith, C.L., Getting, S.J., Perretti, M., Le Tissier, P. and Murray, J.F. 2011. Total pituitary hormone content in wild type and MC3 null male and female mice. British Society for Neuroendocrinology. Cambridge 3rd - 5th July 2011
Comparison between the total pituitary hormone content in wild type and melanocortin 3 receptor (MC3) null male and female mice
Dowejko, M.M., Smith, C.L., Getting, S.J., Perretti, M., Le Tissier, P. and Murray, J.F. 2011. Comparison between the total pituitary hormone content in wild type and melanocortin 3 receptor (MC3) null male and female mice. Society for Reproduction and Fertility. Brighton 11th - 13th July 2011
Do soup preloads reduce total calorific intake in overweight and obese subjects outside the laboratory? A pilot study
Bailey, J. and Murray, J.F. 2011. Do soup preloads reduce total calorific intake in overweight and obese subjects outside the laboratory? A pilot study. International Journal of Food, Nutrition and Public Health. 4 (2), pp. 147-166.
Activation of testicular melanocortin 3 receptors (MC3-R) inhibits LH-stimulated testosterone production
Akindele, K., Horanin, M.M., Getting, S.J., Smith, C.L. and Murray, J.F. 2010. Activation of testicular melanocortin 3 receptors (MC3-R) inhibits LH-stimulated testosterone production. Society for Endocrinology BES 2010. Manchester 15th - 18th March 2010
Circulating melanin concentrating hormone (MCH) concentrations in the menstrual cycle
Naufahu, J., Cunliffe, A. and Murray, J.F. 2010. Circulating melanin concentrating hormone (MCH) concentrations in the menstrual cycle. Society for Reproduction and Fertility. Sutton Bonnington 11th - 13th July 2010
Expression of endogenous and exogenous hypoxic markers in sheep preovulatory antral follicles
Murray, J.F., Gillies, R.M., Carter, N.D. and Robinson, S.P. 2010. Expression of endogenous and exogenous hypoxic markers in sheep preovulatory antral follicles. Society for Reproduction and Fertility. Sutton Bonnington 11th - 13th July 2010
Testicular LH-stimulated testosterone production is inhibited by activation of melanocortin 3 receptors (MC3)
Akindele, K., Horanin, M.M., Getting, S.J., Smith, C.L. and Murray, J.F. 2010. Testicular LH-stimulated testosterone production is inhibited by activation of melanocortin 3 receptors (MC3). Society for Reproduction and Fertility. Sutton Bonnington 11th - 13th July 2010
The antrums of ovine ovarian preovulatory follicles are severely hypoxic
Murray, J.F., Gillies, R.M., Carter, N.D. and Robinson, S.P. 2009. The antrums of ovine ovarian preovulatory follicles are severely hypoxic. Proceedings of the Society For Reproduction and Fertility Annual Conference. Oxford 12-14 July 2009 pp. 38
Identification of a sub-population of pituitary lactotroph cells in mice required for the normal luteotrophic function of prolactin
Jaubert, A., Fernadez-Fuente, M., Carmignac, D., Murray, J.F., Christain, H. and Le Tissier, P. 2009. Identification of a sub-population of pituitary lactotroph cells in mice required for the normal luteotrophic function of prolactin. ENDO2009. Washington 10-13 June 2009
Molecular characterization of the melanocortin 3 receptor in the testes of mice
Horanin, M.M., Akindele, K., Getting, S.J., Smith, C.L. and Murray, J.F. 2009. Molecular characterization of the melanocortin 3 receptor in the testes of mice. pA2 Online. From the Queen Elizabeth II Conference Centre London, Winter 2009 Meeting: Proceedings of the British Pharmacological Society. 7 (4). https://doi.org/http://www.pa2online.org/abstracts/Vol7Issue4abst089P.pdf
Intraovarian changes associated with hypoxia during the rat oestrous cycle
Gillies, R.M., Carter, N.D., Robinson, S.P., McPhail, L.D. and Murray, J.F. 2009. Intraovarian changes associated with hypoxia during the rat oestrous cycle. National Ovarian Workshop. Fitzwilliam College, Cambridge 12-13 September 2009.
Inflamed phenotype of the mesenteric microcirculation of melanocortin type 3 receptor-null mice after ischemia-reperfusion
Leoni, G., Patel, H.B., Sampaio, A.L.F., Gavins, F.N.E., Murray, J.F., Grieco, P., Getting, S.J. and Perretti, M. 2008. Inflamed phenotype of the mesenteric microcirculation of melanocortin type 3 receptor-null mice after ischemia-reperfusion. FASEB Journal. 22 (12), pp. 4228-4238. https://doi.org/10.1096/fj.08-113886
Melanocortin 3 receptor in the testes of mice
Yogarajah, S., Lynch, S., Smith, C.L., Getting, S.J., Perretti, M. and Murray, J.F. 2008. Melanocortin 3 receptor in the testes of mice. Society for Reproduction and Fertility. Edinburgh 29 Jun - 01 Jul 2008 pp. Abstract P80
Melanocortin 3 receptor in the testes of mice
Wotherspoon, G., Yogarajah, S., Lynch, S., Smith, C.L., Getting, S.J., Perretti, M. and Murray, J.F. 2008. Melanocortin 3 receptor in the testes of mice. pA2 Online. From the University of Brighton, Winter 2008 Meeting: Proceedings of the British Pharmacological Society. 6 (4), p. P008.
Evidence for a stimulatory action of melanin-concentrating hormone on luteinising hormone release involving MCH1 and melanocortin-5 receptors
Murray, J.F., Hahn, J.D., Kennedy, A.R., Small, C.J., Bloom, S.R., Haskell-Luevano, C., Coen, C.W. and Wilson, C.A. 2006. Evidence for a stimulatory action of melanin-concentrating hormone on luteinising hormone release involving MCH1 and melanocortin-5 receptors. Journal of Neuroendocrinology. 18 (3), pp. 157-167. https://doi.org/10.1111/j.1365-2826.2005.01397.x
Effects of maternal undernutrition during early pregnancy on apoptosis regulators in the ovine fetal ovary
Lea, R.G., Andrade, L.P., Rae, M.T., Hannah, L.T., Kyle, C.E., Murray, J.F., Rhind, S.M. and Miller, D.W. 2006. Effects of maternal undernutrition during early pregnancy on apoptosis regulators in the ovine fetal ovary. Reproduction. 131, pp. 113-124. https://doi.org/10.1530/rep.1.00844
Developmental indices of nutritionally induced placental growth restriction in the adolescent sheep
Lea, R.G., Hannah, L.T., Redmer, D.A., Aitken, R.P., Milne, J.S., Fowler, P.A., Murray, J.F. and Wallace, J.M. 2005. Developmental indices of nutritionally induced placental growth restriction in the adolescent sheep. Pediatric Research. 57 (4), pp. 599-604. https://doi.org/10.1203/01.PDR.0000155949.08547.66
Leptin expression in the fetus and placenta during mouse pregnancy
Malik, N.M., Carter, N.D., Wilson, C.A., Scaramuzzi, R.J., Stock, M.J. and Murray, J.F. 2005. Leptin expression in the fetus and placenta during mouse pregnancy. Placenta. 26 (1), pp. 47-52. https://doi.org/10.1016/j.placenta.2004.03.009
Overexpression of dimethylarginine dimethylaminohydrolase enhances tumour hypoxia: an insight into the relationship of hypoxia and angiogenesis In vivo
Kostourou, V., Troy, H., Murray, J.F., Cullis, E.R., Whitley, G.S.J., Griffiths, J.R. and Robinson, S. 2004. Overexpression of dimethylarginine dimethylaminohydrolase enhances tumour hypoxia: an insight into the relationship of hypoxia and angiogenesis In vivo. NeoPlasia. 6 (4), pp. 401-411. https://doi.org/10.1593/neo.04109
Neonatal 5HT activity antagonizes the masculinizing effect of testosterone on the luteinizing hormone release response to gonadal steroids and on brain structures in rats
Murray, J.F., Dakin, C.L., Siddiqui, A., Pellatt, L.J., Ahmed, S., Ormerod, L.J.A., Swan, A.V., Davies, D.C. and Wilson, C.A. 2004. Neonatal 5HT activity antagonizes the masculinizing effect of testosterone on the luteinizing hormone release response to gonadal steroids and on brain structures in rats. European Journal of Neuroscience. 19 (2), pp. 387-395. https://doi.org/10.1111/j.0953-816X.2003.03158.x
Central orexin A has site-specific effects on luteinizing hormone release in female rats
Small, C.J., Goubillon, M.L., Murray, J.F., Siddiqui, A., Grimshaw, S.E., Young, H., Sivanesan, V., Kalamatianos, T., Kennedy, A.R., Coen, C.W., Bloom, S.R. and Wilson, C.A. 2003. Central orexin A has site-specific effects on luteinizing hormone release in female rats. Endocrinology. 144 (7), pp. 3225-3236. https://doi.org/10.1210/en.2002-0041
Measurement of cardiac troponin I in striated muscle using three experimental methods
Fredericks, S., Bainbridge, K., Murray, J.F., Collinson, P.O., Carter, N.D. and Holt, D.W. 2003. Measurement of cardiac troponin I in striated muscle using three experimental methods. Annals of Clinical Biochemistry. 40 (3), pp. 244-248. https://doi.org/10.1258/000456303321610547
Cardiac troponin T and creatine kinase MB content in skeletal muscle of the uremic rat
Fredericks, S., Murray, J.F., Carter, N.D., Chesser, A.M.S., Papachristou, S., Yaqoob, M.M., Collinson, P.O., Gaze, D. and Holt, D.W. 2002. Cardiac troponin T and creatine kinase MB content in skeletal muscle of the uremic rat. Clinical Chemistry. 48 (6), pp. 859-868.
Leptin requirement for conception, implantation, and gestation in the mouse
Malik, N.M., Carter, N.D., Murray, J.F., Scaramuzzi, R.J., Wilson, C.A. and Stock, M.J. 2001. Leptin requirement for conception, implantation, and gestation in the mouse. Endocrinology. 142 (12), pp. 5198-5202.
Cardiac troponin T and creatine kinase MB are not increased in exterior oblique muscle of patients with renal failure
Fredericks, S., Murray, J.F., Bewick, M., Chang, R., Collinson, P.O., Carter, N.D. and Holt, D.W. 2001. Cardiac troponin T and creatine kinase MB are not increased in exterior oblique muscle of patients with renal failure. Clinical Chemistry. 47 (6), pp. 1023-1030.
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