Modulation of a cytoskeletal calpain-like protein induces major transitions in trypanosome morphology

Hayes, P.M., Varga, V., Olego-Fernandez, S., Sunter, J., Ginger, M.L., Gull, K. and Dr Michael Ginger 2014. Modulation of a cytoskeletal calpain-like protein induces major transitions in trypanosome morphology. Journal of Cell Biology. 206 (3), pp. 377-384. doi:10.1083/jcb.201312067

TitleModulation of a cytoskeletal calpain-like protein induces major transitions in trypanosome morphology
AuthorsHayes, P.M., Varga, V., Olego-Fernandez, S., Sunter, J., Ginger, M.L., Gull, K. and Dr Michael Ginger
Abstract

Individual eukaryotic microbes, such as the kinetoplastid parasite Trypanosoma brucei, have a defined size, shape, and form yet transition through life cycle stages, each having a distinct morphology. In questioning the structural processes involved in these transitions, we have identified a large calpain-like protein that contains numerous GM6 repeats (ClpGM6) involved in determining T. brucei cell shape, size, and form. ClpGM6 is a cytoskeletal protein located within the flagellum along the flagellar attachment zone (FAZ). Depletion of ClpGM6 in trypomastigote forms produces cells with long free flagella and a shorter FAZ, accompanied by repositioning of the basal body, the kinetoplast, Golgi, and flagellar pocket, reflecting an epimastigote-like morphology. Hence, major changes in microbial cell form can be achieved by simple modulation of one or a few proteins via coordinated association and positioning of membrane and cytoskeletal components.

JournalJournal of Cell Biology
Journal citation206 (3), pp. 377-384
ISSN0021-9525
Year2014
PublisherRockefeller University Press
Publisher's versionJ Cell Biol-2014-Hayes-377-84-1.pdf
Supplementary data or filesJCB_201312067_sm.pdf
Digital Object Identifier (DOI)doi:10.1083/jcb.201312067
Publication dates
Published04 Aug 2014

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