Constriction of Pulmonary Artery by Peroxide: Role of Ca2+ Release and PKC

Pourmahram, G.E., Snetkov, V.A., Shaifta, Y., Drndarski, S., Knock, G.A., Aaronson, P.I. and Ward, J.P. 2008. Constriction of Pulmonary Artery by Peroxide: Role of Ca2+ Release and PKC. Free Radical Biology & Medicine. 45 (10), pp. 1468-76. https://doi.org/10.1016/j.freeradbiomed.2008.08.020

TitleConstriction of Pulmonary Artery by Peroxide: Role of Ca2+ Release and PKC
TypeJournal article
AuthorsPourmahram, G.E., Snetkov, V.A., Shaifta, Y., Drndarski, S., Knock, G.A., Aaronson, P.I. and Ward, J.P.
Abstract

Reactive oxygen species are implicated in pulmonary hypertension and hypoxic pulmonary vasoconstriction. We examined the effects of low concentrations of peroxide on intrapulmonary arteries (IPA). IPAs from Wistar rats were mounted on a myograph for recording tension and estimating intracellular Ca2+ using Fura-PE3. Ca2+ sensitization was examined in alpha-toxin-permeabilized IPAs, and phosphorylation of MYPT-1 and MLC(20) was assayed by Western blot. Peroxide (30 microM) induced a vasoconstriction with transient and sustained components and equivalent elevations of intracellular Ca2+. The transient constriction was strongly suppressed by indomethacin, the TP-receptor antagonist SQ-29584, and the Rho kinase inhibitor Y-27632, whereas sustained constriction was unaffected. Neither vasoconstriction nor elevation of intracellular Ca2+ was affected by removal of extracellular Ca2+, whereas dantrolene suppressed the former and ryanodine abolished the latter. Peroxide-induced constriction of permeabilized IPAs was unaffected by Y-27632 but abolished by PKC inhibitors; these also suppressed constriction in intact IPAs. Peroxide caused translocation of PKCalpha, but had no significant effect on MYPT-1 or MLC(20) phosphorylation. We conclude that in IPAs peroxide causes transient release of vasoconstrictor prostanoids, but sustained constriction is associated with release of Ca2+ from ryanodine-sensitive stores and a PKC-dependent but Rho kinase- and MLC(20)-independent constrictor mechanism.

KeywordsPulmonary artery
Peroxide
Protein kinase C
Hypoxic pulmonary vasoconstriction
Rho kinase
Ca2+ stores
Free radicals
JournalFree Radical Biology & Medicine
Journal citation45 (10), pp. 1468-76
ISSN0891-5849
Year2008
PublisherElsevier
Digital Object Identifier (DOI)https://doi.org/10.1016/j.freeradbiomed.2008.08.020
PubMed ID18805479
Web address (URL)https://www.sciencedirect.com/science/article/pii/S0891584908004991?via%3Dihub
Publication dates
Published15 Nov 2008

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