AIDS is one of the diseases which attract the attention of scientists across the world as we can’t find a country which is trying to prevent its people from this deadly disease. Till now 126 strains of HIV-1 have been reported around the world, which have been classified into groups A-J. The major difference among these strains is their genetic composition. The values of genetic variation were ranged form 8903 to 9720. B-subtype was found to be more diverse (mean 9381.5 ± 396.34) in comparison to others subtypes. Phylogenetic analysis revealed perfect clustering of subtypes. Further wide genomic variation between subtypes is due to more polymorphic sites; varies from 6538 to 8913. On an all genomic bases, the transition mutation was found to be from 2195 to 5161 and the transversion mutation was found from 4343 to 7682.