Pasternack, A., Coleman, S.K., Jouppila, A., Mottershead, D.G., Lindfors, M., Pasternack, M. and Keinänen, K. 2002. Alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor channels lacking the N-terminal domain. Journal of Biological Chemistry. 277 (51), pp. P49662-49667. https://doi.org/10.1074/jbc.m208349200
| Title | Alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor channels lacking the N-terminal domain. |
|---|---|
| Type | Journal article |
| Authors | Pasternack, A., Coleman, S.K., Jouppila, A., Mottershead, D.G., Lindfors, M., Pasternack, M. and Keinänen, K. |
| Abstract | Ionotropic glutamate receptor (iGluR) subunits contain a ∼400-residue extracellular N-terminal domain (“X domain”), which is sequence-related to bacterial amino acid-binding proteins and to class C G-protein-coupled receptors. The X domain has been implicated in the assembly, transport to the cell surface, allosteric ligand binding, and desensitization in various members of the iGluR family, but its actual role in these events is poorly characterized. We have studied the properties of homomeric α-amino-3-hydroxy-5-methylisoxazolepropionate (AMPA)-selective GluR-D glutamate receptors carrying N-terminal deletions. Our analysis indicates that, surprisingly, transport to the cell surface, ligand binding properties, agonist-triggered channel activation, rapid desensitization, and allosteric potentiation by cyclothiazide can occur normally in the complete absence of the X domain (residues 22–402). The relatively intact ligand-gated channel function of a homomeric AMPA receptor in the absence of the X domain indirectly suggests more subtle roles for this domain in AMPA receptors, e.g. in the assembly of heteromeric receptors and in synaptic protein interactions. |
| Journal | Journal of Biological Chemistry |
| Journal citation | 277 (51), pp. P49662-49667 |
| ISSN | 0021-9258 |
| 1083-351X | |
| Year | 2002 |
| Publisher | Elsevier |
| Publisher's version | License CC BY 4.0 File Access Level Open (open metadata and files) |
| Digital Object Identifier (DOI) | https://doi.org/10.1074/jbc.m208349200 |
| PubMed ID | 12393905 |
| Web address (URL) | http://europepmc.org/abstract/med/12393905 |
| Publication dates | |
| Published | 21 Oct 2002 |