Non-typhoidal Salmonella Dublin: Why should we all care?

Mohammed, M. 2022. Non-typhoidal Salmonella Dublin: Why should we all care? International Colloquium on Infectious Diseases, Epidemiology and Public Health (ICIEP 2022). Online 31 Aug - 01 Sep 2022

TitleNon-typhoidal Salmonella Dublin: Why should we all care?
AuthorsMohammed, M.
TypeConference keynote
Abstract

Non-typhoidal Salmonella serovar Dublin is primarily associated with self-limiting gastrointestinal illness however, it has adapted to cause invasive disease in humans such as bacteremia and septicemia. In this study, whole genome sequencing (WGS) of human Salmonella Dublin strains revealed several virulence factors and mobile genetic elements (MGEs) that may contribute to bacterial virulence and enable the bacteria to cause invasive disease in humans including Gifsy-2 prophage, virulence plasmid and Salmonella pathogenicity islands; SPI-7 harbouring the Vi antigen, SPI-6 and SPI-19 harbouring two different T6SSs and the novel pathogenicity island ST313-GI. Interestingly, no genomic markers were detected that differentiate among invasive and non-invasive isolates therefore comparative transcriptomics will be carried out to identify differentially expressed genes in invasive strains compared with non-invasive strains aiming to identify novel virulence-attenuated strains with a potential for use as vaccine candidates for high-risk groups including children, elderly and immunocompromised patients.
Interestingly, non-typhoidal Salmonella serovars including Dublin have developed multi-drug resistance (MDR) against current antibiotics. Bacteriophage therapy is therefore the hope for the treatment of MDR bacterial infections however one of the key limitations to therapeutic use of phages, is the limited host range of many phages and the ease of development of bacterial resistance to phages. A solution is to develop one or a cocktail of engineered phage that overcome these limitations. An essential step towards this goal is understanding the complex dynamics of bacteria-phage interaction. We therefore use Anderson phage typing scheme as a valuable model system for study of phage-host interaction to characterize all bacterial antiviral systems (including clustered regularly interspaced short palindromic repeat (CRISPRs) loci and CRISPR-associated (Cas) proteins (CRISPR-Cas) immune systems, superinfection exclusion (Sie) and restriction-modification (R-M) systems) as well as phage evasion strategies (including anti-CRISPR). We aim to get new insights into phage biology and strategies for genetic modification of phages and designing effective broad spectrum engineered phages to overcome the limitations of bacteriophages as therapeutic agents.

Year2022
Conference International Colloquium on Infectious Diseases, Epidemiology and Public Health (ICIEP 2022)
Accepted author manuscript
Publication dates
Published31 Aug 2022
File

Related outputs

Marburg virus outbreak in Rwanda – what you need to know
Mohammed, M. 2024. Marburg virus outbreak in Rwanda – what you need to know. The Conversation.

Genomic Analysis of uropathogenic E. coli strains in Egypt
Mohammed, M. 2024. Genomic Analysis of uropathogenic E. coli strains in Egypt. Microbiology Society Annual Conference 2024. Edinburgh 08 - 11 Apr 2024 Microbiology Society.

Developing Phage Therapy by Investigating the Basis of Bacteria-Phage interaction
Mohammed, M. 2024. Developing Phage Therapy by Investigating the Basis of Bacteria-Phage interaction. Research & Innovation 2024: Accelerating Future Drug Discovery. Manchester University, UK 20 - 21 Mar 2024

Dr Manal Mohammed comments on the spread of waterborne disease outbreaks during Gaza war
Mohammed, M. 2023. Dr Manal Mohammed comments on the spread of waterborne disease outbreaks during Gaza war. METRO.

Video: Join us at the University of Westminster to Fight superbugs!
Mohammed, M. 2023. Video: Join us at the University of Westminster to Fight superbugs!

Air pollution linked with global rise in antibiotic resistance.
Mohammed, M. 2023. Air pollution linked with global rise in antibiotic resistance. The Conversation.

Investigating the Dynamics of Host-Phage interaction: Towards Effective Phage Therapy!
Mohammed, M. 2023. Investigating the Dynamics of Host-Phage interaction: Towards Effective Phage Therapy! 5th International Conference on Applied Microbiology and Beneficial Microbes. London 09 - 10 Oct 2023

Alternative forms of assessment - the oral tradition
Smith, C.L. and Mohammed, M. 2023. Alternative forms of assessment - the oral tradition. Westminster Learning and Teaching Symposium 2023: Better Than the Real Thing? Exploring Education Futures at the University of Westminster. University of Westminster 04 Sep 2023

Dr Manal Mohammed,has launched a campaign to raise awareness of superbugs (antibiotic resistant bacteria).
Mohammed, M. 2023. Dr Manal Mohammed,has launched a campaign to raise awareness of superbugs (antibiotic resistant bacteria).

Groupwork: Advantages & Disadvantages. A Students as Co-Creators Project
Mohammed, Manal, Agarkar, Ishwari and Begum, Kamnoor 2023. Groupwork: Advantages & Disadvantages. A Students as Co-Creators Project. University of Westminster.

Arcturus: what to know about the new COVID variant, omicron XBB.1.16
Mohammed, M. 2023. Arcturus: what to know about the new COVID variant, omicron XBB.1.16. The Conversation.

Genomic analysis of Anderson typing phages of Salmonella Typhimrium: towards understanding the basis of bacteria-phage interaction
Mohammed, M., Casjens, S.R., Millard, A.D., Harrison, C., Gannon, L. and Chattaway, M.A. 2023. Genomic analysis of Anderson typing phages of Salmonella Typhimrium: towards understanding the basis of bacteria-phage interaction. Scientific Reports. 13 10484. https://doi.org/10.1038/s41598-023-37307-6

Genomic Analysis of Clinical Multidrug Resistant E. coli in Nigerian Hospitals
Mohammed, M., Otong, M.M., Mboto, C., Asuquo, B.S., Okoro, B., Ekpo, C., Okon, U.E. and Mbim, E.N. 2023. Genomic Analysis of Clinical Multidrug Resistant E. coli in Nigerian Hospitals. Microbiology Society Annual Conference 2023. Birmingham, UK 17 - 20 Apr 2023

Difference Festival: Fighting superbugs
Mohammed, M. 2023. Difference Festival: Fighting superbugs. University of Westminster.

Dr Manal Mohammed for The Conversation on the norovirus
Mohammed, M. 2023. Dr Manal Mohammed for The Conversation on the norovirus. The Conversation.

Dr Manal Mohammed for CNN on how often you should wear your clothes without washing them
Mohammed, M. 2023. Dr Manal Mohammed for CNN on how often you should wear your clothes without washing them. CNN.

Dr Manal Mohammed on the new Omicron BF.7 variant for The Conversation
Mohammed, M. 2022. Dr Manal Mohammed on the new Omicron BF.7 variant for The Conversation. The Conversation.

Dr Manal Mohammed on the new Omicron BQ.1 and BQ.1.1 subvariants for The Conversation
Mohammed, M. 2022. Dr Manal Mohammed on the new Omicron BQ.1 and BQ.1.1 subvariants for The Conversation. The Conversation.

Genetic basis of phage-host interaction: Towards effective phage therapy of non-typhoidal Salmonella
Mohammed, M. 2022. Genetic basis of phage-host interaction: Towards effective phage therapy of non-typhoidal Salmonella. Research & Innovation ELRIG 2022. London 04 - 05 Oct 2022

Dr Manal Mohammed on the new Omicron BA.4.6 variant for The Conversation
Mohammed, M. 2022. Dr Manal Mohammed on the new Omicron BA.4.6 variant for The Conversation. The Conversation.

Dr Manal Mohammed for The Conversation on the new ‘bivalent’ COVID-19 booster vaccine
Mohammed, M. 2022. Dr Manal Mohammed for The Conversation on the new ‘bivalent’ COVID-19 booster vaccine. The Conversation.

Dr Manal Mohammed for The Conversation about why eating fermented food aids gut health
Mohammed, M. 2022. Dr Manal Mohammed for The Conversation about why eating fermented food aids gut health. The Conversation.

Dr Manal Mohammed for Which? Travel about how to best protect your health when travelling
Mohammed, M. 2021. Dr Manal Mohammed for Which? Travel about how to best protect your health when travelling. Which?.

Dr Manal Mohammed for CNN about how often you should wash your towels
Mohammed, M. 2021. Dr Manal Mohammed for CNN about how often you should wash your towels. CNN.

CNN Health: How frequently should you wash your bed sheets? More often than you think
Mohammed, M. 2021. CNN Health: How frequently should you wash your bed sheets? More often than you think. CNN.

Dr Manal Mohammed for The Conversation about bed hygiene.
Mohammed, M. 2021. Dr Manal Mohammed for The Conversation about bed hygiene. The Conversation.

Investigating the difference in phage susceptibility among MDR Salmonella Typhimurium phage types DT104 and DT104b.
Mohammed, M. and Orzechowska, B. 2021. Investigating the difference in phage susceptibility among MDR Salmonella Typhimurium phage types DT104 and DT104b. Microbiology Society Annual Conference 2021. Online 26 - 30 Apr 2021

Characterisation of Phage Susceptibility Variation in Salmonellaenterica Serovar Typhimurium DT104 and DT104b
Mohammed, M. and Orzechowska, B. 2021. Characterisation of Phage Susceptibility Variation in Salmonellaenterica Serovar Typhimurium DT104 and DT104b. MDPI Microorganisms. 9 (4) 865. https://doi.org/10.3390/microorganisms9040865

Dr Manal Mohammed for The Conversation about the long-term problems of hand sanitiser
Mohammed, M. 2021. Dr Manal Mohammed for The Conversation about the long-term problems of hand sanitiser. The Conversation.

Stop Superbugs!
Mohammed, M. 2021. Stop Superbugs!

Dr Manal Mohammed for The Independent on handwashing and hand-sanitisers
Mohammed, M. Forthcoming. Dr Manal Mohammed for The Independent on handwashing and hand-sanitisers. The Conversation.

Erratum for Cadel-Six et al., “Draft Genome Sequences of Salmonella enterica subsp. enterica Serovar Dublin Strains from Raw Milk Cheeses Characterized by Multilocus Variable-Number Tandem-Repeat Analysis Profiles Associated with Two Outbreaks in France”
Cadel-Six, Sabrina, Vignaud, Marie-Leone and Mohammed, Manal 2020. Erratum for Cadel-Six et al., “Draft Genome Sequences of Salmonella enterica subsp. enterica Serovar Dublin Strains from Raw Milk Cheeses Characterized by Multilocus Variable-Number Tandem-Repeat Analysis Profiles Associated with Two Outbreaks in France”. Microbiology Resource Announcements. 9 (7). https://doi.org/10.1128/mra.01590-19

Viruses Can Also Wear the White Hats
Mohammed, M. 2020. Viruses Can Also Wear the White Hats. BBVA OpenMind.

Manal Mohammed for Which? about the common mistakes that people make when using hand sanitiser
Mohammed, M. 2020. Manal Mohammed for Which? about the common mistakes that people make when using hand sanitiser. Which?.

Sputnik Radio in Edinburgh: UK Effort to Secure Pfizer Vaccine is Good News but Will Not Give Immediate Protection, Says Expert Dr Manal Mohammed
Mohammed, M. 2020. Sputnik Radio in Edinburgh: UK Effort to Secure Pfizer Vaccine is Good News but Will Not Give Immediate Protection, Says Expert Dr Manal Mohammed. Sputnik Radio in Edinburgh with Craig Johnstone.

Dr Manal Mohammed for The Conversation about the Pfizer vaccine and what to expect next
Mohammed, M. 2020. Dr Manal Mohammed for The Conversation about the Pfizer vaccine and what to expect next. The Conversation.

Dr Manal Mohammed for The Conversation on three major scientific controversies about coronavirus
Mohammed, M. 2020. Dr Manal Mohammed for The Conversation on three major scientific controversies about coronavirus. The Conversation.

Evaluation of WGS-subtyping methods for epidemiological surveillance of foodborne salmonellosis
Mohammed, M. and Thapa, S. 2020. Evaluation of WGS-subtyping methods for epidemiological surveillance of foodborne salmonellosis. One Health Outlook. 2 13 (2020). https://doi.org/10.1186/s42522-020-00016-5

Dr Manal Mohammed holds public lecture about superbugs at Westminster’s Difference Festival (27 February 2020)
Mohammed, M. 2020. Dr Manal Mohammed holds public lecture about superbugs at Westminster’s Difference Festival (27 February 2020).

Dr Manal Mohammed for The Conversation about which kinds of sanitisers are most effective against coronavirus
Mohammed, M. 2020. Dr Manal Mohammed for The Conversation about which kinds of sanitisers are most effective against coronavirus. The Conversation.

Dr Manal Mohammed for the Daily Mail about whether face masks can help stop the spread of viruses (11 Feburary 2020)
Mohammed, M. 2020. Dr Manal Mohammed for the Daily Mail about whether face masks can help stop the spread of viruses (11 Feburary 2020).

The Conversation (Manal Mohammed & Andrew Millard): Antibiotic resistance: Scientists are reengineering viruses to cure bacterial infections
Mohammed, M. and Millard, A. 2020. The Conversation (Manal Mohammed & Andrew Millard): Antibiotic resistance: Scientists are reengineering viruses to cure bacterial infections. The Conversation.

BBC Radio: Manal Mohammed for BBC Radio London on getting the flu at Christmas (17 December 2019).
Mohammed, M. 2019. BBC Radio: Manal Mohammed for BBC Radio London on getting the flu at Christmas (17 December 2019).

The Conversation: Kombucha, kimchi and yogurt: how fermented foods could be harmful to your health.
Mohammed, M. 2019. The Conversation: Kombucha, kimchi and yogurt: how fermented foods could be harmful to your health. The Conversation.

The War between Bacteria and Bacteriophages
Orzechowska, B. and Mohammed, M. 2019. The War between Bacteria and Bacteriophages. in: Growing and Handling of Bacterial Cultures [Working Title] InTech Publishing.

The Conversation: Can surgical masks protect you from getting the flu?
Mohammed, M. 2019. The Conversation: Can surgical masks protect you from getting the flu? The Conversation.

Breaking the wall of Salmonella outbreaks investigation (1/10/2019)
Thapa, S. and Mohammed, M. 2019. Breaking the wall of Salmonella outbreaks investigation (1/10/2019). Falling Walls Lab. London 01 - 01 Oct 2019

BBC Radio London: Dr Manal Mohammed discusses children’s personal hygiene as children go back to school (2 September 2019)
Mohammed, M. 2019. BBC Radio London: Dr Manal Mohammed discusses children’s personal hygiene as children go back to school (2 September 2019).

ITV News: Dr Manal Mohammed discusses bacteria that are resistant to drugs
Mohammed, M. 2019. ITV News: Dr Manal Mohammed discusses bacteria that are resistant to drugs.

Plasma non-termal: teknologi baru yang dapat membunuh 99,9% kuman mematikan di udara
Mohammed, M. 2019. Plasma non-termal: teknologi baru yang dapat membunuh 99,9% kuman mematikan di udara.

The Conversation: Non-thermal plasma: new technology could kill 99.9% of the deadly germs in the air.
Mohammed, M. 2019. The Conversation: Non-thermal plasma: new technology could kill 99.9% of the deadly germs in the air. The Conversation.

Characterization of the Mobilome of Invasive Non-typhoidal Salmonella Dublin
Mohammed, M. 2019. Characterization of the Mobilome of Invasive Non-typhoidal Salmonella Dublin. The Microbiology Society. ICC Belfast, UK 08 - 11 Apr 2019

Characterisation of Prophages in Salmonella Typhimurium DT8
Thapa, S. and Mohammed, M. 2019. Characterisation of Prophages in Salmonella Typhimurium DT8. The Microbiology Society. ICC Belfast, UK 08 - 11 Apr 2019

Novel Approaches for Treatment and Prevention of Invasive Non-typhoidal Salmonella
Mohammed, M. 2019. Novel Approaches for Treatment and Prevention of Invasive Non-typhoidal Salmonella. Research & Innovation ELRIG 2019. Homerton College, Cambridge 02 - 03 Apr 2019

Daily Mail: The perils of sharing popcorn (and earphones, lip salve and even the sofa)! How bacteria, fungi and viruses can give you a very nasty surprise
Mohammed, M. 2019. Daily Mail: The perils of sharing popcorn (and earphones, lip salve and even the sofa)! How bacteria, fungi and viruses can give you a very nasty surprise.

Whole genome sequences of two Salmonella Dublin strains harbour viaA, viaB and ompB loci of the Vi antigen
Mohammed, M., Vignaud, M.-L. and Cadel-Six, S. 2019. Whole genome sequences of two Salmonella Dublin strains harbour viaA, viaB and ompB loci of the Vi antigen . Microbiology Resource Announcements. 8 (14), pp. e00028-19 e00028-19. https://doi.org/10.1128/MRA.00028-19

BBC News: Victoria Derbyshire: Dr Manal Mohammed Comments on Superbugs and Antibiotic Resistance
Mohammed, M. 2019. BBC News: Victoria Derbyshire: Dr Manal Mohammed Comments on Superbugs and Antibiotic Resistance.

Draft Genome Sequences of Salmonella enterica subsp. enterica Serovar Dublin Strains from St. Nectaire and Morbier Cheeses Characterized by Multilocus Variable-Number Tandem-Repeat Analysis Profiles Associated with Two Fatal Outbreaks in France
Six, S.C., Vignaud, M.L. and Mohammed, M. 2019. Draft Genome Sequences of Salmonella enterica subsp. enterica Serovar Dublin Strains from St. Nectaire and Morbier Cheeses Characterized by Multilocus Variable-Number Tandem-Repeat Analysis Profiles Associated with Two Fatal Outbreaks in France. Microbiology Resource Announcements. 8 (1) e01361-18. https://doi.org/10.1128/MRA.01361-18

Novel Insights Into the prevention and Treatment of Invasive Salmonella Dublin
Mohammed, M. 2018. Novel Insights Into the prevention and Treatment of Invasive Salmonella Dublin. 7th European Clinical Microbiology Congress. London, UK 01 - 02 Nov 2018

Novel Insights Into the prevention and Treatment of Invasive Salmonella Dublin. (1-2 November 2018): Invited speaker and congress moderator at the 7th European Clinical Microbiology congress. London, UK.
Mohammed, M. 2018. Novel Insights Into the prevention and Treatment of Invasive Salmonella Dublin. (1-2 November 2018): Invited speaker and congress moderator at the 7th European Clinical Microbiology congress. London, UK. 7th European Clinical Microbiology Congress. London, UK 01 - 02 Nov 2018

Old school wins: outbreak investigation of foodborne salmonellosis
Mohammed, M. 2018. Old school wins: outbreak investigation of foodborne salmonellosis. Atlas of Science.

Atlas of Science: Old School Wins: outbreak investigation of foodborne Salmonellosis
Mohammed, M. 2018. Atlas of Science: Old School Wins: outbreak investigation of foodborne Salmonellosis.

BBC Radio London: Dr Manal Mohammed talks about research finding 19 types of bacteria on cash and coins
Mohammed, M. 2018. BBC Radio London: Dr Manal Mohammed talks about research finding 19 types of bacteria on cash and coins.

BBC Radio London: Dr Manal Mohammed talks about spread of germs in public spaces
Mohammed, M. 2018. BBC Radio London: Dr Manal Mohammed talks about spread of germs in public spaces.

Keynote Speech: Towards Treatment and Prevention of Invasive Non-typhoidal Salmonella
Mohammed, M. 2018. Keynote Speech: Towards Treatment and Prevention of Invasive Non-typhoidal Salmonella. International Conference on Advanced Microbiology and Education. London, UK 14 - 16 Jun 2018

Who Fights Whom? Understanding the Complex Dynamics of Bacteria-Phage Interaction using Anderson Phage Typing System
Mohammed, M. 2018. Who Fights Whom? Understanding the Complex Dynamics of Bacteria-Phage Interaction using Anderson Phage Typing System. Journal of Infectious Diseases & Therapy. 6 (3), p. 367 367. https://doi.org/10.4172/2332-0877.1000367

Phage typing or CRISPR typing for epidemiological surveillance of Salmonella Typhimurium?
Mohammed, M. 2017. Phage typing or CRISPR typing for epidemiological surveillance of Salmonella Typhimurium? BMC Research Notes. 10, p. 578 578. https://doi.org/10.1186/s13104-017-2878-0

Why should Britain Care about Salmonella Dublin?
Mohammed, M. 2017. Why should Britain Care about Salmonella Dublin?

Whole genome sequencing reveals the secrets of Salmonella Dublin invasome
Mohammed, M., Le Hello, S., Leekitcharoenphon, P. and Hendriksen, R. 2017. Whole genome sequencing reveals the secrets of Salmonella Dublin invasome. European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). Vienna, Austria 25 Apr - 21 Jul 2017

The invasome of Salmonella Dublin as revealed by whole genome sequencing
Mohammed, M., Le Hello, S., Leekitcharoenphon, P. and Hendriksen, R. 2017. The invasome of Salmonella Dublin as revealed by whole genome sequencing . BMC Infectious Diseases. 17, p. 544 544. https://doi.org/10.1186/s12879-017-2628-x

Whole Genome Sequencing Provides Insights into the Genetic Determinants of Invasiveness in Salmonella Dublin
Mohammed, M. and Cormican, M. 2016. Whole Genome Sequencing Provides Insights into the Genetic Determinants of Invasiveness in Salmonella Dublin. Epidemiology and Infection. 144 (11), pp. 2430-2439. https://doi.org/10.1017/S0950268816000492

Whole genome sequencing provides an unambiguous link between Salmonella Dublin outbreak strain and a historical isolate
Mohammed, M., Delappe, N., O'Connor, J., McKeown, P., Garvey, P. and Cormican, M. 2016. Whole genome sequencing provides an unambiguous link between Salmonella Dublin outbreak strain and a historical isolate. Epidemiology and Infection. 144 (3), pp. 576-581. https://doi.org/10.1017/S0950268815001636

Whole genome sequencing provides possible explanations for the difference in phage susceptibility among two Salmonella Typhimurium phage types (DT8 and DT30) associated with a single foodborne outbreak.
Mohammed, M. and Cormican, M. 2015. Whole genome sequencing provides possible explanations for the difference in phage susceptibility among two Salmonella Typhimurium phage types (DT8 and DT30) associated with a single foodborne outbreak. BMC Research Notes. 8, p. 728 728. https://doi.org/10.1186/s13104-015-1687-6

Salmonella occurrence and Enterobacteriaceae counts in pig feed ingredients and compound feed from feed mills in Ireland
Burns, A.M., Lawlor, P.G., Gardiner, G.E., McCabe, E.M., Walsh, D., Mohammed, M., Grant, J. and Duffy, G. 2015. Salmonella occurrence and Enterobacteriaceae counts in pig feed ingredients and compound feed from feed mills in Ireland. Preventive Veterinary Medicine. 121 (3-4), pp. 231-239. https://doi.org/10.1016/j.prevetmed.2015.07.002

Permalink - https://westminsterresearch.westminster.ac.uk/item/vz6w9/non-typhoidal-salmonella-dublin-why-should-we-all-care


Share this

Usage statistics

82 total views
56 total downloads
These values cover views and downloads from WestminsterResearch and are for the period from September 2nd 2018, when this repository was created.