The fungal alkaloid Okaramine-B activates an L-glutamate-gated chloride channel from Ixodes scapularis, a tick vector of Lyme disease

Furutani, S., Ihara, M., Lees, K., Buckingham, S.D., Partridge, F.A., David, J.A., Patel, R., Warchal, S., Mellor, I.R., Matsuda, K. and Sattelle, D.B. 2018. The fungal alkaloid Okaramine-B activates an L-glutamate-gated chloride channel from Ixodes scapularis, a tick vector of Lyme disease. International Journal for Parasitology: Drugs and Drug Resistance. 8 (2), pp. 350-360. https://doi.org/10.1016/j.ijpddr.2018.06.001

TitleThe fungal alkaloid Okaramine-B activates an L-glutamate-gated chloride channel from Ixodes scapularis, a tick vector of Lyme disease
TypeJournal article
AuthorsFurutani, S., Ihara, M., Lees, K., Buckingham, S.D., Partridge, F.A., David, J.A., Patel, R., Warchal, S., Mellor, I.R., Matsuda, K. and Sattelle, D.B.
Abstract

A novel L-glutamate-gated anion channel (IscaGluCl1) has been cloned from the black-legged tick, Ixodes scapularis, which transmits multiple pathogens including the agents of Lyme disease and human granulocytic anaplasmosis. When mRNA encoding IscaGluCl1 was expressed in Xenopus laevis oocytes, we detected robust 50–400 nA currents in response to 100 μM L-glutamate. Responses to L-glutamate were concentration-dependent (pEC50 3.64 ± 0.11). Ibotenate was a partial agonist on IscaGluCl1. We detected no response to 100 μM aspartate, quisqualate, kainate, AMPA or NMDA. Ivermectin at 1 μM activated IscaGluCl1, whereas picrotoxinin (pIC50 6.20 ± 0.04) and the phenylpyrazole fipronil (pIC50 6.90 ± 0.04) showed concentration-dependent block of the L-glutamate response. The indole alkaloid okaramine B, isolated from fermentation products of Penicillium simplicissimum (strain AK40) grown on okara pulp, activated IscaGluCl1 in a concentration-dependent manner (pEC50 5.43 ± 0.43) and may serve as a candidate lead compound for the development of new acaricides.

JournalInternational Journal for Parasitology: Drugs and Drug Resistance
Journal citation8 (2), pp. 350-360
ISSN2211-3207
Year2018
PublisherElsevier
Publisher's version
License
CC BY 4.0
File Access Level
Open (open metadata and files)
Digital Object Identifier (DOI)https://doi.org/10.1016/j.ijpddr.2018.06.001
Web address (URL)https://doi.org/10.1016/j.ijpddr.2018.06.001
Publication dates
PublishedAug 2018

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