Xiaoan Wu, Kevin P. Cunningham, Andrew Bruening-Wright, Shilpi Pandey and H. Peter Larsson 2024. Preprint: Loose Coupling between the Voltage Sensor and the Activation Gate in Mammalian HCN Channels Suggests a Gating Mechanism. Preprints.org. https://doi.org/10.20944/preprints202403.0499.v1
| Title | Preprint: Loose Coupling between the Voltage Sensor and the Activation Gate in Mammalian HCN Channels Suggests a Gating Mechanism |
|---|---|
| Authors | Xiaoan Wu, Kevin P. Cunningham, Andrew Bruening-Wright, Shilpi Pandey and H. Peter Larsson |
| Description | Voltage-gated potassium (Kv) channels and hyperpolarization-activated cyclic nucleotide-gated (HCN) channels share similar structures but have opposite gating polarity. Kv channels have a strong coupling ( >109) between the voltage sensor (S4) and the activation gate: when S4s are activated the gate is open to >80%, but when S4s are deactivated, the gate is open < 10-9 of the time. Using noise analysis, we show that the coupling between S4 and the gate is < 200 in HCN channels. In addition, using voltage clamp fluorometry, locking the gate open in a Kv channel drastically altered the energetics of S4 movement. In contrast, locking the gate open or decreasing the coupling between S4 and the gate in HCN channels had only minor effects on the energetics of S4 movement, consistent with a weak coupling between S4 and the gate. We propose that this loose coupling is a prerequisite for the reversed voltage gating in HCN channels. |
| Year | 2024 |
| Output media | Preprints.org |
| Publisher | Preprints.org |
| Publication dates | |
| Published | 08 Mar 2024 |
| Digital Object Identifier (DOI) | https://doi.org/10.20944/preprints202403.0499.v1 |
| Web address (URL) | https://doi.org/10.20944/preprints202403.0499.v1 |