In vitro activities of Kissorphin, a novel hexapeptide KiSS-1 derivative, in neuronal cells

Milton, N.G.N. 2012. In vitro activities of Kissorphin, a novel hexapeptide KiSS-1 derivative, in neuronal cells. Journal of Amino Acids. 2012, p. Article ID 691463. doi:10.1155/2012/691463

TitleIn vitro activities of Kissorphin, a novel hexapeptide KiSS-1 derivative, in neuronal cells
AuthorsMilton, N.G.N.
Abstract

The primary products of the metastasis-suppressor KiSS-1 gene are the kisspeptin (KP) peptides that stimulate gonadotrophin-releasing-hormone (GnRH) release via GPR-54 receptor activation. Recent studies have suggested that the KP-10 peptide also activates neuropeptide FF (NPFF) receptors. The aim of the current study was to determine the activities of the KiSS-1 derivative kissorphin (KSO), which contains the first six amino acids of the KP-10 peptide, is C-terminally amidated, and shares amino acid similarities with the biologically active NPFF 3–8 sequence. The KSO peptide inhibited forskolin-stimulated cyclic adenosine monophosphate (cAMP) production in ND7/23 neuroblastoma cells via an action that could be inhibited by the NPFF receptor antagonist RF9. Release of GnRH by LA-N-1 neuroblastoma cells was not altered by the KSO peptide. In ND7/23 neuroblastoma cells, the KSO peptide was able to reduce forskolin neuroprotection against H2O2 toxicity. The KSO peptide was also able to prevent prostaglandin E2-induced apoptosis in rat cortical neurons. The NPFF receptor antagonist RF9 could inhibit these actions of the KSO peptide in oxidative stress and apoptosis models. In conclusion, the kissorphin peptide, comprising the amino acid sequence Tyr-Asn-Trp-Asn-Ser-Phe-NH2, has NPFF-like biological activity without showing any GnRH releasing activity and inhibits forskolin-activated cAMP release.

JournalJournal of Amino Acids
Journal citation2012, p. Article ID 691463
ISSN2090-0104
Year2012
PublisherHindawi
FileMilton_2012_as_published.pdf
Digital Object Identifier (DOI)doi:10.1155/2012/691463
Publication dates
Published2012

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