|Title||Transcriptome analysis shows activation of circulating CD8+ T cells in patients with severe asthma|
|Authors||Tsitsiou, E., Williams, A.E., Moschos, S.A., Patel, K., Rossios, C., Jiang, X., Adams, O.D., Macedo, P., Booton, R., Gibeon, D., Chung, K.F. and Lindsay, M.A.|
Background - Although previous studies have implicated tissue CD4+ T cells in the development and maintenance of the inflammatory response in asthmatic patients, little is known about the role of CD8+ T cells. There is now accumulating evidence that microRNAs and other noncoding RNAs are important regulators of T-cell function.
We sought to use transcriptomics to determine the activation state of circulating CD4+ and CD8+ T cells in patients with nonsevere and severe asthma.
mRNA and noncoding RNA expression in circulating T cells was measured by means of microarray, quantitative real-time PCR, or both.
Results - Comparison of mRNA expression showed widespread changes in the circulating CD8+ but not CD4+ T cells from patients with severe asthma. No changes were observed in the CD4+ and CD8+ T cells in patients with nonsevere asthma versus those in healthy control subjects. Bioinformatics analysis showed that the changes in CD8+ T-cell mRNA expression were associated with multiple pathways involved in T-cell activation. As with mRNAs, we also observed widespread changes in expression of noncoding RNA species, including natural antisense, pseudogenes, intronic long noncoding RNAs (lncRNAs), and intergenic lncRNAs in CD8+ T cells from patients with severe asthma. Measurement of the microRNA expression profile showed selective downregulation of miR-28-5p in CD8+ T cells and reduction of miR-146a and miR-146b in both CD4+ and CD8+ T cells.
Severe asthma is associated with the activation of circulating CD8+ T cells but not CD4+ T cells. This response is correlated with the downregulation of miR-146a/b and miR-28-5p, as well as changes in the expression of multiple species of lncRNA that might regulate CD8+ T-cell function.
|Journal||Journal of Allergy and Clinical Immunology|
|Journal citation||129 (1), pp. 95-103|
|Digital Object Identifier (DOI)||doi:10.1016/j.jaci.2011.08.011|