In situ hybridisation: Technologies and their application to understanding disease

Warford, A. 2016. In situ hybridisation: Technologies and their application to understanding disease. Progress in Histochemistry and Cytochemistry. 50 (4), pp. 37-48.

TitleIn situ hybridisation: Technologies and their application to understanding disease
AuthorsWarford, A.
Abstract

In situ hybridisation (ISH) is unique amongst molecular analysis methods in providing for the precise microscopic localisation of genes, mRNA and microRNA in metaphase spreads,cell and tissue preparations. The method is well established as a tool to guide appropriate therapeutic intervention in breast, gastric and lung cancer. With the description of ultrasensitive ISH technologies for low copy mRNA demonstration and the relative ease by which microRNA can be visualised, the applications for research and diagnostic purposes is set to increase dramatically. In this review ISH is considered with emphasis on recent technological developments and surveyed for present and future applications in the context of the demonstration of genes, mRNA and microRNA in health and disease.

KeywordsIn situ hybridisation,DNA amplification, mRNA expression, microRNA localisationa
JournalProgress in Histochemistry and Cytochemistry
Journal citation50 (4), pp. 37-48
ISSN0079-6336
Year2016
PublisherElsevier
Digital Object Identifier (DOI)doi:10.1016/j.proghi.2015.12.001
Publication dates
PublishedJan 2016
Published25 Dec 2016

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