Antibody validation of immunohistochemistry for biomarker discovery: Recommendations of a consortium of academic and pharmaceutical based histopathology researchers

Howat, W.J., Lewis, A., Jones, P., Kampf, C., Pontén, F., F, van der Loos, C.M., Gray, N., Womack, C. and Warford, A. 2014. Antibody validation of immunohistochemistry for biomarker discovery: Recommendations of a consortium of academic and pharmaceutical based histopathology researchers. Methods. 70 (1), pp. 34-38.

TitleAntibody validation of immunohistochemistry for biomarker discovery: Recommendations of a consortium of academic and pharmaceutical based histopathology researchers
AuthorsHowat, W.J., Lewis, A., Jones, P., Kampf, C., Pontén, F., F, van der Loos, C.M., Gray, N., Womack, C. and Warford, A.
Abstract

As biomarker discovery takes centre-stage, the role of immunohistochemistry within that process is increasing. At the same time, the number of antibodies being produced for ‘‘research use’’ continues to rise and it is important that antibodies to be used as biomarkers are validated for specificity and sensitivity before use. This guideline seeks to provide a stepwise approach for the validation of an antibody for immunohistochemical assays, reflecting the views of a consortium of academic and pharmaceutical based histopathology researchers. We propose that antibodies are placed into a tier system, level 1–3, based on evidence of their usage in immunohistochemistry, and that the degree of validation required is proportionate to their place on that tier.

KeywordsAntibody validation Immunohistochemistry Biomarker Guidelines Histopathology
JournalMethods
Journal citation70 (1), pp. 34-38
ISSN1046-2023
Year2014
PublisherElsevier
Accepted author manuscript1-s2.0-S1046202314000280-main.pdf
Digital Object Identifier (DOI)doi:10.1016/j.ymeth.2014.01.018
Publication dates
PublishedNov 2014
Published11 Feb 2014
LicenseCC BY-NC-SA 3.0

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