Antigen retrieval, blocking, detection and visualisation systems in immunohistochemistry: A review and practical evaluation of tyramide and rolling circle amplification systems.

Warford, A., Akbar, H. and Riberio, D. 2014. Antigen retrieval, blocking, detection and visualisation systems in immunohistochemistry: A review and practical evaluation of tyramide and rolling circle amplification systems. Methods. 70 (1), p. 28–33.

TitleAntigen retrieval, blocking, detection and visualisation systems in immunohistochemistry: A review and practical evaluation of tyramide and rolling circle amplification systems.
AuthorsWarford, A., Akbar, H. and Riberio, D.
Abstract

To achieve specificity and sensitivity using immunohistochemistry it is necessary to combine the
application of validated primary antibodies with optimised pre-treatment, detection and visualisation
steps. The influence of these surrounding procedures is reviewed. A practical evaluation of tyramide
signal amplification and rolling circle amplification detection methods is provided in which formalin
fixed paraffin embedded sections of adenocarcinomas of breast, colon and lung together with squamous
metaplasia of lung were immunostained with CD20 and CK19 primary antibodies. The results indicate
that the detection systems are of comparable sensitivity and specificity.

KeywordsImmunohistochemistry Antigen retrieval Detection systems Tyramide signal amplification Rolling circle amplification
JournalMethods
Journal citation70 (1), p. 28–33
ISSN1046-2023
Year2014
PublisherElsevier
Digital Object Identifier (DOI)doi:10.1016/j.ymeth.2014.03.001
Publication dates
PublishedNov 2014
Published12 Mar 2014

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