Increased expression of transglutaminase-1 and PPARgamma after vitamin E treatment in human keratinocytes

De Pascale, M.C., Bassi, A.M., Patrone, V., Villacorta, L., Azzi, A. and Zing, J.M. 2006. Increased expression of transglutaminase-1 and PPARgamma after vitamin E treatment in human keratinocytes. Archives of Biochemistry and Biophysics. 447 (2), pp. 97-106. https://doi.org/10.1016/j.abb.2006.02.002

TitleIncreased expression of transglutaminase-1 and PPARgamma after vitamin E treatment in human keratinocytes
AuthorsDe Pascale, M.C., Bassi, A.M., Patrone, V., Villacorta, L., Azzi, A. and Zing, J.M.
Abstract

In skin, vitamin E acts as the predominant lipophilic antioxidant with a protective function against irradiation and oxidative stress. In addition to that, vitamin E can also modulate signal transduction and gene expression. To study whether the four natural tocopherol analogues (α-, β-, γ-, δ-tocopherol) can influence transcriptional activity by modulating the activity of nuclear receptors, a human keratinocytes cell line (NCTC 2544) was transfected with plasmids containing the luciferase reporter gene under control by direct repeat elements (DR1–DR4), representing binding sites for four different classes of nuclear receptors. In this model, the tocopherols positively modulated only the reporter construct containing a consensus element for peroxisome proliferator-activated receptors (PPARs). The induction was strongest with γ-tocopherol and was most likely the direct consequence of stimulation of PPARγ protein expression in keratinocytes. Vitamin E treatment also led to increased expression of a known PPARγ target gene involved in terminal keratinocytes differentiation, the transglutaminase-1.

JournalArchives of Biochemistry and Biophysics
Journal citation447 (2), pp. 97-106
ISSN0003-9861
Year2006
PublisherElsevier
Digital Object Identifier (DOI)https://doi.org/10.1016/j.abb.2006.02.002
Publication dates
Published15 Mar 2006

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