Antioxidant status in J774A.1 macrophage cell line during chronic exposure to glycated serum

Bassi, A.M., Ledda, S., De Pascale, M.C., Penco, S., Rossi, S., Odetti, P. and Cottalasso, D. 2005. Antioxidant status in J774A.1 macrophage cell line during chronic exposure to glycated serum. Biochemistry and Cell Biology. 83 (2), pp. 176-87.

TitleAntioxidant status in J774A.1 macrophage cell line during chronic exposure to glycated serum
AuthorsBassi, A.M., Ledda, S., De Pascale, M.C., Penco, S., Rossi, S., Odetti, P. and Cottalasso, D.
Abstract

Advanced glycation end-products (AGEs) are linked to aging and correlated diseases. The aim of present study was to evaluate oxidative stress related parameters in J774A.1 murine macrophage cells during chronic exposure to a subtoxic concentration of AGE (5% ribose-glycated serum (GS)) and subsequently for 48 h to a higher dose (10% GS). No effects on cell viability were evident in either experimental condition. During chronic treatment, glycative markers (free and bound pentosidine) increased significantly in intra- and extracellular environments, but the production and release of thiobarbituric acid reactive substances (TBARs), as an index of lipid peroxidation, underwent a time-dependent decrease. Exposure to 10% GS evidenced that glycative markers rose further, while TBARs elicited a cellular defence against oxidative stress. Nonadapted cultures showed an accumulation of AGEs, a marked oxidative stress, and a loss of viability. During 10% GS exposure, reduced glutathione levels in adapted cultures remained constant, as did the oxidized glutathione to reduced glutathione ratio, while nonadapted cells showed a markedly increased redox ratio. A constant increase of heat shock protein 70 (HSP70) mRNA was observed in all experimental conditions. On the contrary, HSP70 expression became undetectable for a longer exposure time; this could be due to the direct involvement of HSP70 in the refolding of damaged proteins. Our findings suggest an adaptive response of macrophages to subtoxic doses of AGE, which could constitute an important factor in the spread of damage to other cellular types during aging.Key words: in vitro cytotoxicity, AGE, pentosidine, glycoxidation, oxidative stress, TBARs.

JournalBiochemistry and Cell Biology
Journal citation83 (2), pp. 176-87
ISSN0829-8211
Year2005
PublisherNRC Research Press
Digital Object Identifier (DOI)doi:10.1139/o05-024
Publication dates
PublishedApr 2005

Related outputs

Influence of chylomicron remnants on human monocyte activation in vitro
Bentley, C., Hathaway, N., Widdows, J., Bejta, F., De Pascale, C., Avella, M., Wheeler-Jones, C.P.D., Botham, K.M. and Lawson, C. 2011. Influence of chylomicron remnants on human monocyte activation in vitro. Nutr Metab Cardiovasc Dis.. 21 (11), pp. 871-878.

Suppression of nuclear factor-kappaB activity in macrophages by chylomicron
De Pascale, C., Graham, V., Fowkes, R.C., Wheeler-Jones, C.P. and Botham, K.M. 2010. Suppression of nuclear factor-kappaB activity in macrophages by chylomicron. European Atherosclerosis Society Meeting . Hamburg, germany 20 - 24 Jun 2010 Elsevier.

P221 lipid droplets in human macrophages recruit cytosolic phospholipase A2 and promote secretion of inflammatory mediators
De Pascale, C., Bostrom, P., Wickström, Y., Mogensen, C., MattssonHultén, L., Perkins, R., Ståhlman, M., Wiklund, O., Olofsson, S.-O. and Borén, J. 2010. P221 lipid droplets in human macrophages recruit cytosolic phospholipase A2 and promote secretion of inflammatory mediators. European Atherosclerosis Society (EAS) Conference. Hamburg 20 - 24 Jun 2010 Elsevier.

Modulation of CYP1A1 by PKC Inhibitors and TPA Pre-Treatments in MH1C1 Rat Hepatoma Cells Exposed to 3 -Methylcholanthrene
De Pascale, C., Domenicotti, C., Nitti, M., Marengo, B., Catalano, M., Scanarotti, C., Sanguineti, R., Siri, M., Ledda, S., Penco, S. and Bassi, A. 2009. Modulation of CYP1A1 by PKC Inhibitors and TPA Pre-Treatments in MH1C1 Rat Hepatoma Cells Exposed to 3 -Methylcholanthrene. The Open Toxicology Journal . (3), pp. 47-57.

Suppression of nuclear factor-κB activity in macrophages by chylomicron remnants: modulation by the fatty acid composition of the particles
De Pascale, C., Graham, V., Fowkes, R.C., Wheeler-Jones, C.P.D. and Botham, K.M. 2009. Suppression of nuclear factor-κB activity in macrophages by chylomicron remnants: modulation by the fatty acid composition of the particles. FEBS Journal. 276 (19), pp. 5689-5702.

Characterization of three human sec14p-like proteins: a-Tocopherol
Zingg, J.M., Kempna, P., Paris, M., Reiter, E., Villacorta, L., Cipollone, R., Munteanu, A., De Pascale, C., Menini, S., Cueff, A., Arock, M., Azzi, A. and Ricciarelli, R. 2008. Characterization of three human sec14p-like proteins: a-Tocopherol. Biochimie . 90 (11-12), p. 1703–1715.

The induction of macrophages foam cell formation by chylomicron remnants
Botham, K.M., Moore, E.H., De Pascale, C. and Bejta, F. 2007. The induction of macrophages foam cell formation by chylomicron remnants. Biochemical Society Transactions. 35 (3), pp. 454-458.

Dietary fats induce human monocyte activation in vitro
Bentley, C., Bejta, F., De Pascale, C., Avella, M., Wheeler-Jones, C.P.D., Botham, K.M. and Lawson, C. 2007. Dietary fats induce human monocyte activation in vitro. Biochemical Society Transactions. 35 (3), pp. 464-465.

Effects of lycopene on the induction of foam cell formation by modified LDL.
Napolitano, M., De Pascale, C., Wheeler-Jones, C., Botham, K.M. and Bravo, E. 2007. Effects of lycopene on the induction of foam cell formation by modified LDL. American Journal of Physiology: Endocrinology and Metabolism. 293 (6), pp. E1820-7.

Increased expression of transglutaminase-1 and PPARgamma after vitamin E treatment in human keratinocytes
De Pascale, M.C., Bassi, A.M., Patrone, V., Villacorta, L., Azzi, A. and Zing, J.M. 2006. Increased expression of transglutaminase-1 and PPARgamma after vitamin E treatment in human keratinocytes. Archives of Biochemistry and Biophysics. 447 (2), pp. 97-106.

Fatty acids composition of Chylomicron remnants like particles influences their uptake and induction of lipid accumulation in macrophages.
De Pascale, C., Avella, M., Perona, J.S., Ruiz-Gutierrez, V., Wheeler-Jones, C.P.D. and Botham, K.M. 2006. Fatty acids composition of Chylomicron remnants like particles influences their uptake and induction of lipid accumulation in macrophages. FEBS Journal . 273 (24), p. 5632–5640.

Damaging effects of advanced glycation end product in the murine macrophages cell line J774A.1.
Bassi, A.M., Ledda, S., Valentini, S., De Pascale, M.C., Rossi, S., Odetti, P. and Cottalasso, D. 2002. Damaging effects of advanced glycation end product in the murine macrophages cell line J774A.1. Toxicology In Vitro. 16 (4), pp. 339-347.

Permalink - https://westminsterresearch.westminster.ac.uk/item/9y20y/antioxidant-status-in-j774a-1-macrophage-cell-line-during-chronic-exposure-to-glycated-serum


Share this
Tweet
Email