John Mina, Y. Okada, Nilu Wansadhipathi-Kannangara, S. Pratt, H. Shams-Eldin, Ralph T. Schwarz, P. G. Steel, Tony Fawcett and Paul Denny 2010. Functional analyses of differentially expressed isoforms of the Arabidopsis Inositol phosphorylceramide synthase. Plant molecular biology. 73 (4-5), pp. 399-407. https://doi.org/10.1007/s11103-010-9626-3
| Title | Functional analyses of differentially expressed isoforms of the Arabidopsis Inositol phosphorylceramide synthase |
|---|---|
| Type | Journal article |
| Authors | John Mina, Y. Okada, Nilu Wansadhipathi-Kannangara, S. Pratt, H. Shams-Eldin, Ralph T. Schwarz, P. G. Steel, Tony Fawcett and Paul Denny |
| Abstract | Sphingolipids are key components of eukaryotic plasma membranes that are involved in many functions, including the formation signal transduction complexes. In addition, these lipid species and their catabolites function as secondary signalling molecules in, amongst other processes, apoptosis. The biosynthetic pathway for the formation of sphingolipid is largely conserved. However, unlike mammalian cells, fungi, protozoa and plants synthesize inositol phosphorylceramide (IPC) as their primary phosphosphingolipid. This key step involves the transfer of the phosphorylinositol group from phosphatidylinositol (PI) to phytoceramide, a process catalysed by IPC synthase in plants and fungi. This enzyme activity is at least partly encoded by the AUR1 gene in the fungi, and recently the distantly related functional orthologue of this gene has been identified in the model plant Arabidopsis. Here we functionally analysed all three predicted Arabidopsis IPC synthases, confirming them as aureobasidin A resistant AUR1p orthologues. Expression profiling revealed that the genes encoding these orthologues are differentially expressed in various tissue types isolated from Arabidopsis. |
| Keywords | inositol phosphorylceramide |
| Aureobasidin | |
| Phosphorylinositol | |
| Sphingolipids | |
| Arabidopsis thaliana | |
| Trypanosoma brucei | |
| Journal | Plant molecular biology |
| Journal citation | 73 (4-5), pp. 399-407 |
| ISSN | 1573-5028 |
| 0167-4412 | |
| Year | 2010 |
| Publisher | Springer |
| Digital Object Identifier (DOI) | https://doi.org/10.1007/s11103-010-9626-3 |
| Web address (URL) | https://link.springer.com/article/10.1007/s11103-010-9626-3 |
| Publication dates | |
| Published | 23 Mar 2010 |
| Supplemental file | File Access Level Controlled (open metadata, closed files) |