Comparison of the two different recombinant proteins representing region II of the Duffy binding protein of Plasmodium vivax by assaying for natural antibodies

Nilu Wansadhipathi-Kannangara, Nilu Wansadhipathi, P H Premarathne, W T A Wickremarachchi, K L R L Perera, S Bandara, S M Handunnetti and P V Udagama-Randeniya 2005. Comparison of the two different recombinant proteins representing region II of the Duffy binding protein of Plasmodium vivax by assaying for natural antibodies. Allergy and Immunology Society of Sri Lanka (and FIMSA). Medical Research Council, Sri Lanka 03 - 05 Jan 2005

TitleComparison of the two different recombinant proteins representing region II of the Duffy binding protein of Plasmodium vivax by assaying for natural antibodies
AuthorsNilu Wansadhipathi-Kannangara, Nilu Wansadhipathi, P H Premarathne, W T A Wickremarachchi, K L R L Perera, S Bandara, S M Handunnetti and P V Udagama-Randeniya
TypeConference paper
Abstract

Two different recombinant proteins representing region II of the Duffy Binding Protein of Plasmodium vivax, DBP and PvRII expressed in the bacculovirus and Eschericia coli vector
systems, respectively, were compared by assaying the total immunoglobulin (IgM + IgG) responses of sera of patients with acute vivax malaria in an indirect ELISA. The patients were
from two malaria endemic areas, Anuradhapura (n=64) and Kataragama (n=90), and a nonendemic area, Colombo (n=90).
The antibody prevalence was 50% and 44% from Anuradhapura, 39% and 28% Kataragama and 57% and 41% from Colombo, for PvRII and DBP, respectively. The antibody prevalence for PvRII was higher than that for DBP in each test area, that was significant only in Colombo (p=0.001). The percentages of patients that they responded to both proteins were 34% (n=22), 19% (n=17) and 40% (n=36) from Anuradhapura, Kataragama and from Colombo, respectively. In comparison, a significantly lower (p=0.007) percentage of individuals from Kataragama responded to both proteins. Further 16% (n=10) from Anuradhapura, 19% (n=17) from Kataragama and 16% (n=14) from Colombo preferentially recognised PvRII, whereas, corresponding values for DBP were 9% (n=6), 10% (n=9) and 1% (n=1), respectively, where this difference was significant only in Colombo (p=0.031). Among the previously non-exposed patients from Colombo, 24% responded preferentially to PvRII whereas it was only 3% for DBP (p=0.021).On the other hand, of the previously exposed patients from Colombo, 10% preferentially responded to PvRII whereas no preferential recognition of DBP was observed (p=0.063).

Thus the results of this study show a higher natural antibody response to recombinant protein PvRII, which represents the functional conformation of region II of the Duffy Binding
Protein.

We are thankful to Dr Chitnis, Malaria Division, ICGEB, New Delhi, India and Dr S Longacre, Department of Immunology, Institute Pasteur, France for kindly providing the two
recombinant proteins. Financial support by the International Foundation for Science, Sweden (Grant No: F3008-1) and University of Colombo (Grant No: 2001/S/23) are acknowledged.

KeywordsPlasmodium vivax
Malaria
Duffy binding protein
Natural antibodies
recombinant proteins
Year2005
ConferenceAllergy and Immunology Society of Sri Lanka (and FIMSA)

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