Paschinger, K., Hykollari, A., Razzazi-Fazeli, E., Greenwell, P., Leitsch, D., Walochnik, J. and Wilson, I.B.H. 2012. The N-glycans of Trichomonas vaginalis contain variable core and antennal modifications. Glycobiology. 22 (2), pp. 300-313. https://doi.org/10.1093/glycob/cwr149
| Title | The N-glycans of Trichomonas vaginalis contain variable core and antennal modifications |
|---|---|
| Authors | Paschinger, K., Hykollari, A., Razzazi-Fazeli, E., Greenwell, P., Leitsch, D., Walochnik, J. and Wilson, I.B.H. |
| Abstract | Trichomonad species are widespread unicellular flagellated parasites of vertebrates which interact with their hosts through carbohydrate–lectin interactions. In the past, some data have been accumulated regarding their lipo(phospho)glycans, a major glycoconjugate on their cell surfaces; on the other hand, other than biosynthetic aspects, few details about their N-linked oligosaccharides are known. In this study, we present both mass spectrometric and high-performance liquid chromatography data about the N-glycans of different strains of Trichomonas vaginalis, a parasite of the human reproductive tract. The major structure in all strains examined is a truncated oligomannose form (Man5GlcNAc2) with α1,2-mannose residues, compatible with a previous bioinformatic examination of the glycogenomic potential of T. vaginalis. In addition, dependent on the strain, N-glycans modified by pentose residues, phosphate or phosphoethanolamine and terminal N-acetyllactosamine (Galβ1,4GlcNAc) units were found. The modification of N-glycans by N-acetyllactosamine in at least some strains is shared with the lipo(phospho)glycan and may represent a further interaction partner for host galectins, thereby playing a role in binding of the parasite to host epithelia. On the other hand, the variation in glycosylation between strains may be the result of genetic diversity within this species. |
| Journal | Glycobiology |
| Journal citation | 22 (2), pp. 300-313 |
| ISSN | 0959-6658 |
| Year | Feb 2012 |
| Publisher | Oxford University Press |
| Digital Object Identifier (DOI) | https://doi.org/10.1093/glycob/cwr149 |
| Publication dates | |
| Published | Feb 2012 |
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