|Title||Detection of human papillomavirus from liquid-based cytology specimens by in-house PCR: a pilot study|
|Authors||Rughooputh, S., Parmar, K. and Greenwell, P.|
The Papanicolaou smear remains the most common method for the detection of precancerous changes in cervical cytology. However, the introduction of a liquidbased cytology (LBC) technique expands the possibility of cervical intraepithelial neoplasia (CIN) diagnosis, and permits detection of precancerous changes and human papillomavirus (HPV) simultaneously. In the pilot study reported here, using an in-house polymerase chain reaction (PCR) method, high-grade HPV was detected in 32% of a cohort of 38 patients. This conventional PCR method could be developed for use on a real-time PCR platform or in a microtitre-well format and subsequently automated.
|Keywords||Cervical intraepithelial neoplasia, Cervical neoplasia. Human papillomavirus, Liquid-based cytology, Polymerase chain reaction|
|Journal||British Journal of Biomedical Science|
|Journal citation||61 (1), pp. 22-25|
|Web address (URL)||http://www.bjbs-online.org/article.asp?id=131|