Structural basis for nick recognition by a minimal pluripotent DNA ligase

Nair, P.A., Nandakumar, J., Smith, P., Odell, M., Lima, C.D. and Shuman, S. 2007. Structural basis for nick recognition by a minimal pluripotent DNA ligase. Nature Structural and Molecular Biology. 14 (8), pp. 770-778.

TitleStructural basis for nick recognition by a minimal pluripotent DNA ligase
AuthorsNair, P.A., Nandakumar, J., Smith, P., Odell, M., Lima, C.D. and Shuman, S.
Abstract

Chlorella virus DNA ligase, the smallest eukaryotic ligase known, has pluripotent biological activity and an intrinsic nick-sensing function, despite having none of the accessory domains found in cellular ligases. A 2.3-Å crystal structure of the Chlorella virus ligase-AMP intermediate bound to duplex DNA containing a 3'-OH–5'-PO4 nick reveals a new mode of DNA envelopment, in which a short surface loop emanating from the OB domain forms a -hairpin 'latch' that inserts into the DNA major groove flanking the nick. A network of interactions with the 3'-OH and 5'-PO4 termini in the active site illuminates the DNA adenylylation mechanism and the crucial roles of AMP in nick sensing and catalysis. Addition of a divalent cation triggered nick sealing in crystallo, establishing that the nick complex is a bona fide intermediate in the DNA repair pathway.

JournalNature Structural and Molecular Biology
Journal citation14 (8), pp. 770-778
ISSN1545-9993
YearAug 2007
Digital Object Identifier (DOI)doi:10.1038/nsmb1266
Publication dates
PublishedAug 2007

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