Arabidopsis AtPARK13, which confers thermotolerance, targets misfolded proteins

Basak, I., Pal, R., Patil, K.S., Dunne, A., Ho, H.P., Lee, S., Peiris, D., Maple-Grødem, J., Odell, M., Chang, E.J., Larsen, J.P. and Møller, S. 2014. Arabidopsis AtPARK13, which confers thermotolerance, targets misfolded proteins. Journal of Biological Chemistry. 289, pp. 14458-14469. https://doi.org/10.1074/jbc.M114.548156

TitleArabidopsis AtPARK13, which confers thermotolerance, targets misfolded proteins
AuthorsBasak, I., Pal, R., Patil, K.S., Dunne, A., Ho, H.P., Lee, S., Peiris, D., Maple-Grødem, J., Odell, M., Chang, E.J., Larsen, J.P. and Møller, S.
Abstract

Mutations in HTRA2/Omi/PARK13 have been implicated in Parkinson disease (PD). PARK13 is a neuroprotective serine protease; however, little is known about how PARK13 confers stress protection and which protein targets are directly affected by PARK13. We have reported that Arabidopsis thaliana represents a complementary PD model, and here we demonstrate that AtPARK13, similar to human PARK13 (hPARK13), is a mitochondrial protease. We show that the expression/accumulation of AtPARK13 transcripts are induced by heat stress but not by other stress conditions, including oxidative stress and metals. Our data show that elevated levels of AtPARK13 confer thermotolerance in A. thaliana. Increased temperatures accelerate protein unfolding, and we demonstrate that although AtPARK13 can act on native protein substrates, unfolded proteins represent better AtPARK13 substrates. The results further show that AtPARK13 and hPARK13 can degrade the PD proteins α-synuclein (SNCA) and DJ-1/PARK7 directly, without autophagy involvement, and that misfolded SNCA and DJ-1 represent better substrates than their native counterparts. Comparative proteomic profiling revealed AtPARK13-mediated proteome changes, and we identified four proteins that show altered abundance in response to AtPARK13 overexpression and elevated temperatures. Our study not only suggests that AtPARK13 confers thermotolerance by degrading misfolded protein targets, but it also provides new insight into possible roles of this protease in neurodegeneration.

JournalJournal of Biological Chemistry
Journal citation289, pp. 14458-14469
ISSN0021-9258
Year2014
PublisherAmerican Society for Microbiology and Molecular Biology
Digital Object Identifier (DOI)https://doi.org/10.1074/jbc.M114.548156
Publication dates
Published2014

Related outputs

Fatty acid metabolism of Mycobacterium tuberculosis: a double-edged sword
Gaspar Quinonez, C., Lee, J.J., Lim, J., Odell, M., Lawson, C.P., Anyogu, A., Raheem, S. and Eoh, H. 2022. Fatty acid metabolism of Mycobacterium tuberculosis: a double-edged sword. mBio. 13 (1) e03559-21. https://doi.org/10.15698/mic2022.05.777

The Role of Fatty Acid Metabolism in Drug Tolerance of Mycobacterium tuberculosis
Gaspar Quinonez, C., Lee, J.J., Lim, J., Odell, M., Lawson, C.P., Anyogu, A., Raheem, S. and Eoh, H. 2022. The Role of Fatty Acid Metabolism in Drug Tolerance of Mycobacterium tuberculosis. mBio. 13 (1), pp. e03559-21. https://doi.org/10.1128/mbio.03559-21

Glutamate mediated metabolic neutralization mitigates propionate toxicity in intracellular Mycobacterium tuberculosis
Lee, J.J., Lim, J., Gao, S., Lawson, C.P., Odell, M., Raheem, S., Woo, J., Kang, S-H., Kang, S-S., Jeon, B-Y. and Eoh, H. 2018. Glutamate mediated metabolic neutralization mitigates propionate toxicity in intracellular Mycobacterium tuberculosis. Scientific Reports. 8 8506. https://doi.org/10.1038/s41598-018-26950-z

Identification of O-Linked Glycoproteins Binding to the Lectin Helix pomatia Agglutinin as Markers of Metastatic Colorectal Cancer
Peiris, D., Ossondo, M., Fry, S., Loizidou, M., Smith-Ravin, J. and Dwek, M. 2015. Identification of O-Linked Glycoproteins Binding to the Lectin Helix pomatia Agglutinin as Markers of Metastatic Colorectal Cancer. PLoS ONE. 10 (10) e0138345. https://doi.org/10.1371/journal.pone.0138345

ROS-mediated DJ-1 monomerization modulates intracellular trafficking involving Karyopherin-2
Bjørkblom, B., Maple-Grødem, J., Puno, M.R., Odell, M., Larsen, J.P. and Møller, S. 2014. ROS-mediated DJ-1 monomerization modulates intracellular trafficking involving Karyopherin-2. Molecular and Cellular Biology. 34 (16), pp. 3024-3040. https://doi.org/10.1128/MCB.00286-14

Arabidopsis AtPARK13, which confers thermotolerance, targets misfolded proteins
Basak, I., Pal, R., Patil, K.S., Dunne, A., Ho, H.P., Lee, S., Peiris, D., Maple-Grødem, J., Odell, M., Chang, E.J., Larsen, J.P. and Møller, S. 2014. Arabidopsis AtPARK13, which confers thermotolerance, targets misfolded proteins. Journal of Biological Chemistry. 289 (21), pp. 14458-69.

Proteomics analysis of Bacillus licheniformis in response to oligosaccharides elicitors
Reffatti, P.F., Roy, I., Odell, M. and Keshavarz, T. 2014. Proteomics analysis of Bacillus licheniformis in response to oligosaccharides elicitors. Enzyme Microb Technol. . 61-62, pp. 61-66. https://doi.org/10.1016/j.enzmictec.2014.03.007

Evidence for the involvement of intracellular Ca 2+ ions in the elicitation mechanism of Bacillus Licheniformis
Reffatti, P., Roy, I., Odell, M. and Keshavarz, T. 2013. Evidence for the involvement of intracellular Ca 2+ ions in the elicitation mechanism of Bacillus Licheniformis. Journal of Molecular Microbiology and Biotechnology. OnlineFirst. https://doi.org/10.1159/000351515

The neuroprotective role of catalase overexpression in SH-SY5Y cells against beta-amyloid and H2O2 toxicity
Chilumuri, A., Odell, M. and Milton, N.G.N. 2013. The neuroprotective role of catalase overexpression in SH-SY5Y cells against beta-amyloid and H2O2 toxicity. Alzheimer's & Dementia. 9 (4), p. P361.

Structure of Cu(I)-bound DJ-1 reveals a biscysteinate metal binding site at the homodimer interface: insights into mutational inactivation of DJ-1 in parkinsonism
Puno, M.R., Patel, N.A., Møller, S.G., Robinson, C.V., Moody, P.C.E. and Odell, M. 2013. Structure of Cu(I)-bound DJ-1 reveals a biscysteinate metal binding site at the homodimer interface: insights into mutational inactivation of DJ-1 in parkinsonism. Journal of the American Chemical Society. 135 (43), p. 15974–15977. https://doi.org/10.1021/ja406010m

Benzothiazole aniline-tetra(ethylene glycol) and 3-amino-1,2,4-triazole inhibit neuroprotection against amyloid peptides by catalase overexpression in vitro
Chilumuri, A., Odell, M. and Milton, N.G.N. 2013. Benzothiazole aniline-tetra(ethylene glycol) and 3-amino-1,2,4-triazole inhibit neuroprotection against amyloid peptides by catalase overexpression in vitro. ACS chemical neuroscience. 4 (11), pp. 1501-1512. https://doi.org/10.1021/cn400146a

Identification of metastasis-associated glycoproteins in colorectal cancer
Peiris, D., Markiv, A., Curley, G.P. and Dwek, M. 2012. Identification of metastasis-associated glycoproteins in colorectal cancer. European Journal of Cancer. 48 (5), pp. S39-S40. https://doi.org/10.1016/S0959-8049(12)70863-8

Is quorum sensing involved in lovastatin production in the filamentous fungus Aspergillus terreus?
Raina, S., De Vizio, D., Palonen, E.K., Odell, M., Brandt, A.M., Soini, J.T. and Keshavarz, T. 2012. Is quorum sensing involved in lovastatin production in the filamentous fungus Aspergillus terreus? Process Biochemistry. 47 (5), pp. 843-852. https://doi.org/10.1016/j.procbio.2012.02.021

The mycelial response of the white-rot fungus, Schizophyllum commune to the biocontrol agent, Trichoderma viride
Ujor, V.C., Monti, M., Peiris, D., Clements, M.O. and Hedger, J.N. 2012. The mycelial response of the white-rot fungus, Schizophyllum commune to the biocontrol agent, Trichoderma viride. Fungal Biology. 116 (2), pp. 332-341. https://doi.org/10.1016/j.funbio.2011.12.008

Quantitative proteomic analysis of the response of the wood-rot fungus, Schizophyllum commune to the biocontrol fungus, Trichoderma viride
Ujor, V.C., Peiris, D., Monti, M., Kang, A.S., Clements, M.O. and Hedger, J.N. 2012. Quantitative proteomic analysis of the response of the wood-rot fungus, Schizophyllum commune to the biocontrol fungus, Trichoderma viride. Letters in Applied Microbiology. 54 (4), pp. 336-343. https://doi.org/10.1111/j.1472-765X.2012.03215.x

2DE- based proteomics for the analysis of metastasis associated proteins
Dwek, M. and Peiris, D. 2012. 2DE- based proteomics for the analysis of metastasis associated proteins. Methods in Molecular Biology. 878, pp. 111-120. https://doi.org/10.1007/978-1-61779-854-2_7

A novel approach to determining the affinity of protein-carbohydrate interactions employing adherent cancer cells grown on a biosensor surface
Peiris, D., Markiv, A., Curley, G.P. and Dwek, M. 2012. A novel approach to determining the affinity of protein-carbohydrate interactions employing adherent cancer cells grown on a biosensor surface. Biosensors and Bioelectronics. 35 (1), pp. 160-166. https://doi.org/10.1016/j.bios.2012.02.037

A physiological approach to assess the affinity of lectin carbohydrate interactions using cancer cells immobilised on a biosensor surface
Peiris, D., Markiv, A. and Dwek, M. 2011. A physiological approach to assess the affinity of lectin carbohydrate interactions using cancer cells immobilised on a biosensor surface. Glycobiology. 21 (11), p. 1523. https://doi.org/10.1093/glycob/cwr126

Identification, cloning and characterization of two N-acetylgalactosamine binding lectins from the albumen gland of Helix pomatia
Markiv, A., Peiris, D., Curley, G.P., Odell, M. and Dwek, M. 2011. Identification, cloning and characterization of two N-acetylgalactosamine binding lectins from the albumen gland of Helix pomatia. Journal of Biological Chemistry. 286 (23), pp. 20260-20266. https://doi.org/10.1074/jbc.M110.184515

Quorum sensing as a method for improving sclerotiorin production in Penicillium sclerotiorum
Raina, S., Odell, M. and Keshavarz, T. 2010. Quorum sensing as a method for improving sclerotiorin production in Penicillium sclerotiorum. Journal of Biotechnology. 148 (2-3), pp. 91-98. https://doi.org/10.1016/j.jbiotec.2010.04.009

Cancer prognostication using a recombinant form of the lectin from Helix pomatia agglutinin
Dwek, M., Markiv, A. and Odell, M. 2009. Cancer prognostication using a recombinant form of the lectin from Helix pomatia agglutinin. Glycobiology. 19 (11), p. 1318. https://doi.org/10.1093/glycob/cwp135

Microbial quorum sensing: a tool or a target for antimicrobial therapy?
Raina, S., De Vizio, D., Odell, M., Clements, M.O., Vanhulle, S. and Keshavarz, T. 2009. Microbial quorum sensing: a tool or a target for antimicrobial therapy? Biotechnology and Applied Biochemistry. 54 (2), pp. 65-84. https://doi.org/10.1042/BA20090072

Metabolite profiles of interacting mycelial fronts differ for pairings of the wood decay basidiomycete fungus, Stereum hirsutum with its competitors Coprinus micaceus and Coprinus disseminatus
Peiris, D., Dunn, W.B., Brown, M., Kell, D.B., Roy, I. and Hedger, J.N. 2008. Metabolite profiles of interacting mycelial fronts differ for pairings of the wood decay basidiomycete fungus, Stereum hirsutum with its competitors Coprinus micaceus and Coprinus disseminatus. Metabolomics. 4 (1), pp. 52-62. https://doi.org/10.1007/s11306-007-0100-4

Polyhydroxyalkanoate synthase in Bacillus cereus SPV, a class IV PHA synthase
Philip, S.E., Odell, M., Keshavarz, T. and Roy, I. 2008. Polyhydroxyalkanoate synthase in Bacillus cereus SPV, a class IV PHA synthase. 11th International Symposium on Biodegradable Polyesters. Massey University in Auckland, New Zealand 23 - 26 Nov 2008

Structural basis for nick recognition by a minimal pluripotent DNA ligase
Nair, P.A., Nandakumar, J., Smith, P., Odell, M., Lima, C.D. and Shuman, S. 2007. Structural basis for nick recognition by a minimal pluripotent DNA ligase. Nature Structural and Molecular Biology. 14 (8), pp. 770-778. https://doi.org/10.1038/nsmb1266

Polyhydroxy-alkanoates - the biodegradable polymers
Philip, S.E., Odell, M., Keshavarz, T. and Roy, I. 2007. Polyhydroxy-alkanoates - the biodegradable polymers. International Conference on Biodegradable Polymers: their production, characterisation and application. SCI, London 10 Dec 2007

Polyhydroxyalkanoate (PHA) biosynthesis from structurally unrelated carbon sources by a newly characterized Bacillus spp
Valappil, S.P., Peiris, D., Langley, G.J., Herniman, J.M., Boccaccini, A.R., Bucke, C. and Roy, I. 2007. Polyhydroxyalkanoate (PHA) biosynthesis from structurally unrelated carbon sources by a newly characterized Bacillus spp. Journal of Biotechnology. 127 (3), pp. 475-487. https://doi.org/10.1016/j.jbiotec.2006.07.015

Circadian orchestration of the hepatic proteome
Reddy, A.B., Karp, N.A., Maywood, E.S., Sage, E.A., Deery, M., O'Neill, J.S., Wong, G.K.Y., Chesham, J., Odell, M., Lilley, K.S., Kyriacou, C.P. and Hastings, M.H. 2006. Circadian orchestration of the hepatic proteome. Current Biology. 16 (11), pp. 1107-1115. https://doi.org/10.1016/j.cub.2006.04.026

Polyhydroxyalkanoate synthases: the enzymes involved in biodegradable polymer synthesis
Valappil, S.P., Philip, S.E., Odell, M., Keshavarz, T. and Roy, I. 2005. Polyhydroxyalkanoate synthases: the enzymes involved in biodegradable polymer synthesis. Proceedings of Frontiers in Chemical Biology: Mechanistic Enzymology and Biocatalysis. Exeter, UK 31 Aug - 02 Sep 2005

DNA Ligases: Stuctures
Odell, M. 2004. DNA Ligases: Stuctures. in: Lennarz, W.J. and Lane, M.D. (ed.) Encyclopedia of biological chemistry Amsterdam, Netherlands Elsevier.

No ARM in it? (reply to Kippert and Gerloff)
Kyriacou, C.P. and Odell, M. 2004. No ARM in it? (reply to Kippert and Gerloff). Current Biology. 14 (16), pp. R652-R653. https://doi.org/10.1016/j.cub.2004.08.009

Analysis of the DNA joining repertoire of Chlorella virus DNA ligase and a new crystal structure of the ligase-adenylate intermediate
Odell, M., Malinina, L., Sriskanda, V., Teplova, M. and Shuman, S. 2003. Analysis of the DNA joining repertoire of Chlorella virus DNA ligase and a new crystal structure of the ligase-adenylate intermediate. Nucleic Acids Research. 31 (17), pp. 5090-5100. https://doi.org/10.1093/nar/gkg665

Structure-based experimental confirmation of biochemical function to a methyltransferase, MJ0882, from hyperthermophile Methanococcus jannaschii
Huang, L., Hung, L., Odell, M., Yokota, H., Kim, R. and Kim, S.H. 2002. Structure-based experimental confirmation of biochemical function to a methyltransferase, MJ0882, from hyperthermophile Methanococcus jannaschii. Journal of Structural and Functional Genomics. 2 (3), pp. 121-127. https://doi.org/10.1023/A:1021279113558

The Drosophila clock protein Timeless is a member of the Arm/HEAT family
Vodovar, N., Clayton, J., Costa, R., Odell, M. and Kyriacou, C.P. 2002. The Drosophila clock protein Timeless is a member of the Arm/HEAT family. Current Biology. 12 (18), pp. R610-R611. https://doi.org/10.1016/S0960-9822(02)01130-2

Crystal structure of eukaryotic DNA ligase–adenylate illuminates the mechanism of nick sensing and strand joining
Odell, M., Sriskanda, V., Shuman, S. and Nikolov, D.B. 2000. Crystal structure of eukaryotic DNA ligase–adenylate illuminates the mechanism of nick sensing and strand joining. Molecular Cell. 6 (5), pp. 1183-1193.

Footprinting of Chlorella virus DNA ligase bound at a nick in duplex DNA
Odell, M. and Shuman, S. 1999. Footprinting of Chlorella virus DNA ligase bound at a nick in duplex DNA. Journal of Biological Chemistry. 274 (20), pp. 14032-14039.

Permalink - https://westminsterresearch.westminster.ac.uk/item/964w6/arabidopsis-atpark13-which-confers-thermotolerance-targets-misfolded-proteins


Share this

Usage statistics

217 total views
0 total downloads
These values cover views and downloads from WestminsterResearch and are for the period from September 2nd 2018, when this repository was created.