Laurson, J., Selden, C., Clements, M.O., Mavri-Damelin, D., Coward, S., Lowdell, M. and Hodgson, H.J.F. 2007. Putative human liver progenitor cells in explanted liver. Cells Tissues Organs. 186 (3), pp. 180-191. https://doi.org/10.1159/000106360
| Title | Putative human liver progenitor cells in explanted liver |
|---|---|
| Authors | Laurson, J., Selden, C., Clements, M.O., Mavri-Damelin, D., Coward, S., Lowdell, M. and Hodgson, H.J.F. |
| Abstract | Background/Aims: Hepatocyte progenitors have frequently been cultured from rodents but reports from human liver are rare. Methods: Non-parenchymal cell fraction isolated from 19 explant livers (removed at orthotopic liver transplantation for acute or chronic liver disease) and histologically normal human liver was cultured. Results: Proliferating epithelioid colonies were identifiable after 2-3 weeks culture as a very rare event (<1 per million cells plated) expressing mRNAs and protein antigens of mixed hepatocytic/biliary phenotype. Colony survival could be prolonged by transduction of the catalytic sub-unit of telomerase. Hepatocyte growth factor, epidermal growth factor and oncostatin M did not further enhance hepatocytic differentiation. The expression of markers associated with hepatocyte precursor status was investigated by flow cytometry. Cells expressing the stem cell-associated markers CD133 and CD117 were identified at low frequency. The proportion of cells expressing the integrin CD49f was higher in diseased liver than in normal liver, but the proportion expressing the hepatocyte growth factor receptor c-met was lower. Successful enrichment of plated populations for progenitors was not achieved. Conclusion: Although there is clear histological evidence of hepatocyte precursors in human explant livers, predictable culture of such cells with differentiation toward mature hepatocyte phenotype remains elusive. |
| Journal | Cells Tissues Organs |
| Journal citation | 186 (3), pp. 180-191 |
| ISSN | 1422-6405 |
| Year | 2007 |
| Publisher | S. Karger AG |
| Digital Object Identifier (DOI) | https://doi.org/10.1159/000106360 |
| Publication dates | |
| Published | 2007 |
Overexpression of the Mtr pathway in Shewanella oneidensis for bioelectricity production
Fapetu, S.A., Keshavarz, T., Clements, M.O. and Kyazze, G. 2017. Overexpression of the Mtr pathway in Shewanella oneidensis for bioelectricity production. (Society for Applied Microbiology) 6th ECS Research Symposium. University of Westminster 19 Apr 2017 Society for Applied Microbiology.
Enhancing electricity production from wastewater using microbial fuel cells
Fapetu, S., Keshavarz, T., Clements, M.O. and Kyazze, G. 2016. Enhancing electricity production from wastewater using microbial fuel cells. Postgraduate Fair 2016. NCS, University of Westminster 28 Apr 2016
Enhancing electricity production from wastewater using microbial fuel cells
Fapetu, S., Keshavarz, T., Clements, M.O. and Kyazze, G. 2016. Enhancing electricity production from wastewater using microbial fuel cells. Aulenta, F. and Majone, M. (ed.) EU-ISMET 2016: The 3rd European Meeting of the International Society for Microbial Electrochemistry and Technology. Department of Chemistry (NEC) Sapienza, University of Rome, Rome, Italy 26 - 28 Sep 2016 ISMET.
Contribution of direct electron transfer mechanisms to overall electron transfer in microbial fuel cells utilising Shewanella oneidensis as biocatalyst
Fapetu, S.A., Keshavarz, T., Clements, M.O. and Kyazze, G. 2016. Contribution of direct electron transfer mechanisms to overall electron transfer in microbial fuel cells utilising Shewanella oneidensis as biocatalyst. Biotechnology Letters. 38 (9), pp. 1465-1473. https://doi.org/10.1007/s10529-016-2128-x
Enhancing electricity production in microbial fuel cells using defined co-cultures
Fapetu, S., Keshavarz, T., Clements, M.O. and Kyazze, G. 2015. Enhancing electricity production in microbial fuel cells using defined co-cultures. Postgraduate Fair. New Cavendish Street, London 30 Apr 2015
Application of RT-Bst to enhance detection of pathogenic viruses of the respiratory tract
Kabir, M.S., Clements, M.O., Atkins, M. and Kimmitt, P.T. 2015. Application of RT-Bst to enhance detection of pathogenic viruses of the respiratory tract. British Journal of Biomedical Science. 72 (3), pp. 128-134.
RT-Bst: an integrated approach for reverse transcription and enrichment of cDNA from viral RNA
Kabir, Md. Shahidul, Kimmitt, P.T. and Clements, M.O. 2015. RT-Bst: an integrated approach for reverse transcription and enrichment of cDNA from viral RNA. British Journal of Biomedical Sciences. 72 (1), pp. 1-6.
Campylobacter jejuni gene cj0511 encodes a serine peptidase essential for colonisation
Karlyshev, A.V., Thacker, G., Jones, M.A., Clements, M.O. and Wren, B.W. 2014. Campylobacter jejuni gene cj0511 encodes a serine peptidase essential for colonisation. FEBS Open Biology. 4, pp. 468-472. https://doi.org/10.1016/j.fob.2014.04.012
On - track: A Change Academy project investigating student attendance and engagement
Smith, C.L., Benson, N., Reilly, A.M., Allen, J., Clements, M.O., Shacklady, D., Davies, P., Bacquet, S. and Umeh, K. 2013. On - track: A Change Academy project investigating student attendance and engagement. 12th Annual Westminster Learning and Teaching Symposium. 04 Jul 2013
The role of cancer stem cells in osteosarcoma
Jenks, A., Smith, C.L., Patel, V. and Clements, M.O. 2013. The role of cancer stem cells in osteosarcoma. International society for stem cell research conference. Boston (Massachusetts, USA) June 12 – 15 2013
The mycelial response of the white-rot fungus, Schizophyllum commune to the biocontrol agent, Trichoderma viride
Ujor, V.C., Monti, M., Peiris, D., Clements, M.O. and Hedger, J.N. 2012. The mycelial response of the white-rot fungus, Schizophyllum commune to the biocontrol agent, Trichoderma viride. Fungal Biology. 116 (2), pp. 332-341. https://doi.org/10.1016/j.funbio.2011.12.008
γ-Heptalactone is an endogenously produced quorum-sensing molecule regulating growth and secondary metabolite production by Aspergillus nidulans
Williams, H.E., Steele, J.C.P., Clements, M.O. and Keshavarz, T. 2012. γ-Heptalactone is an endogenously produced quorum-sensing molecule regulating growth and secondary metabolite production by Aspergillus nidulans. Applied Microbiology and Biotechnology. 96 (3), pp. 773-781. https://doi.org/10.1007/s00253-012-4065-5
Quantitative proteomic analysis of the response of the wood-rot fungus, Schizophyllum commune to the biocontrol fungus, Trichoderma viride
Ujor, V.C., Peiris, D., Monti, M., Kang, A.S., Clements, M.O. and Hedger, J.N. 2012. Quantitative proteomic analysis of the response of the wood-rot fungus, Schizophyllum commune to the biocontrol fungus, Trichoderma viride. Letters in Applied Microbiology. 54 (4), pp. 336-343. https://doi.org/10.1111/j.1472-765X.2012.03215.x
Genetic analysis of the pnp–deaD genetic region reveals membrane lipoprotein NlpI as an independent participant in cold acclimatization of Salmonella enterica serovar Typhimurium
Fazle Rouf, S., Anwar, N., Clements, M.O. and Rhen, M. 2011. Genetic analysis of the pnp–deaD genetic region reveals membrane lipoprotein NlpI as an independent participant in cold acclimatization of Salmonella enterica serovar Typhimurium. FEMS Microbiology Letters. 325 (1), pp. 56-63. https://doi.org/10.1111/j.1574-6968.2011.02416.x
The Making Assessment Count (MAC) consortium–maximising assessment and feedback design by working together
Kerrigan, M.J.P., Walker, S., Gamble, M., Lindsay, S., Reader, K., Papaefthimiou, M.C., Newman-Ford, L., Saunders, G. and Clements, M.O. 2011. The Making Assessment Count (MAC) consortium–maximising assessment and feedback design by working together. in: Proceedings of ALT-C: Thriving in a colder and more challenging climate, 6-8 September 2011, University of Leeds Association for Learning Technology.
Microbial quorum sensing: a tool or a target for antimicrobial therapy?
Raina, S., De Vizio, D., Odell, M., Clements, M.O., Vanhulle, S. and Keshavarz, T. 2009. Microbial quorum sensing: a tool or a target for antimicrobial therapy? Biotechnology and Applied Biochemistry. 54 (2), pp. 65-84. https://doi.org/10.1042/BA20090072
The student voice in biosciences
Kerrigan, M.J.P., Clements, M.O. and Rumpus, A. 2009. The student voice in biosciences. University of Westminster Teaching & Learning Symposium. University of Westminster, London 2009 Association for Learning Technology.
Listening to the student voices in biosciences modules
Kerrigan, M.J.P., Clements, M.O. and Rumpus, A. 2009. Listening to the student voices in biosciences modules. Hearing the Student Voice Conference: Enabling students' voices to shape the curriculum. Leeds Metropolitan University 14 May 2009
eReflect: enhancing student support through a reflective online process
Kerrigan, M.J.P., Clements, M.O., Oradini, F., Saunders, G. and Bond, A. 2009. eReflect: enhancing student support through a reflective online process. BbWorld'09. National Harbor, Maryland 14 - 16 Jul 2009
eReflect: making assessment count
Kerrigan, M.J.P., Clements, M.O., Bond, A. and Saunders, G. 2009. eReflect: making assessment count. in: Bracq, A., Jefferies, A.L. and Terry, E.B. (ed.) Proceedings of the Fourth International Blended Learning Conference "Engaging Students in the Curriculum" Hatfield University of Hertfordshire. pp. 219-232
Making assessment count: integrating Web 2.0 to support student assessment and reflection
Kerrigan, M.J.P., Clements, M.O., Bond, A., Oradini, F. and Saunders, G. 2009. Making assessment count: integrating Web 2.0 to support student assessment and reflection. E-learning 2.0. Brunel University, Uxbridge 06 - 07 Jul 2009
Making assessment count – integrating feedback and reflection through eReflect
Kerrigan, M.J.P., Clements, M.O., Bond, A., Oradini, F., Nedelcheva, Y. and Saunders, G. 2009. Making assessment count – integrating feedback and reflection through eReflect. in: Terry, E.B., Jefferies, A.L. and Bracq, A. (ed.) Proceedings of the Fourth International Blended Learning Conference "Engaging Students in the Curriculum" Hatfield University of Hertfordshire.
University of westminster, Making assessment count: a JISC funded project
Kerrigan, M.J.P., Clements, M.O., Bond, A., Nedelcheva, Y. and Saunders, G. 2009. University of westminster, Making assessment count: a JISC funded project. 6th International Conference of the Association for Learning Technology. Manchester, UK 08 - 10 Sep 2009 Association for Learning Technology.
University of Westminster, Making assessment count: a JISC funded project
Kerrigan, M.J.P., Clements, M.O., Bond, A., Nedelcheva, Y. and Saunders, G. 2009. University of Westminster, Making assessment count: a JISC funded project. SSHL: Teaching and Learning Day. University of Westminster, London 2009 Association for Learning Technology.
University of Westminster, Making assessment count: a JISC funded project
Kerrigan, M.J.P., Clements, M.O., Bond, A., Nedelcheva, Y. and Saunders, G. 2009. University of Westminster, Making assessment count: a JISC funded project. University of Westminster Teaching & Learning Symposium. University of Westminster, London 2009 Association for Learning Technology.
The biology of human mesenchymal stem cells
Westwood, C. and Clements, M.O. 2008. The biology of human mesenchymal stem cells. in: Levičar, N., Habib, N., Gordon, M.Y. and Dimarakis, I. (ed.) Stem cell repair and regeneration London Imperial College Press.
Transformation of human mesenchymal stem cells increases their dependency on oxidative phosphorylation for energy production
Funes, J.M., Quintero, M., Henderson, S.R., Martinez, D., Qureshi, U., Westwood, C., Clements, M.O., Bourboulia, D., Pedley, R.B., Moncada, S. and Boshoff, C. 2007. Transformation of human mesenchymal stem cells increases their dependency on oxidative phosphorylation for energy production. Proceedings of the National Academy of Sciences. 104 (15), pp. 6223-6228. https://doi.org/10.1073/pnas.0700690104
Inhibitation of γ-secretases alters both proliferation and differentiation of mesenchymal stem cells
Vujovic, S., Henderson, S.R., Flanagan, A.M. and Clements, M.O. 2007. Inhibitation of γ-secretases alters both proliferation and differentiation of mesenchymal stem cells. Cell Proliferation. 40 (2), pp. 185-195. https://doi.org/10.1111/j.1365-2184.2007.00426.x
Polynucleotide phosphorylase negatively controls spv virulence gene expression in Salmonella enterica
Ygberg, S.E., Clements, M.O., Rytkonen, A., Thompson, A., Holden, D.W., Hinton, J.C.D. and Rhen, M. 2006. Polynucleotide phosphorylase negatively controls spv virulence gene expression in Salmonella enterica. Infection and Immunity. 74 (2), pp. 1243-1254. https://doi.org/10.1128/IAI.74.2.1243-1254.2006
Notch signalling in self-renewal and differentiation of human mesenchymal stem cells
Vujovic, S., Henderson, S.R., Flanagan, A.M. and Clements, M.O. 2006. Notch signalling in self-renewal and differentiation of human mesenchymal stem cells. 1st UK Mesenchymal Stem Cell Meeting. York, UK 08 Jun 2006
Behavior of adult human mesenchymal stem cells entrapped in alginate-GRGDY beads
Markusen, J.F., Mason, C., Hull, D.A., Town, M.A., Tabor, A.B., Clements, M.O., Boshoff, C. and Dunnill, P. 2006. Behavior of adult human mesenchymal stem cells entrapped in alginate-GRGDY beads. Tissue Engineering. 12 (4), pp. 821-830.
Extending the lineage potential of human mesenchymal stem cells
Elliman, S.J., Bryson, K., Trotter, M.W.B., Cellek, S., Kyriakou, C., Yong, K.L., Boshoff, C. and Clements, M.O. 2006. Extending the lineage potential of human mesenchymal stem cells. 1st UK Mesenchymal Stem Cell Meeting. York, UK 08 Jun 2006
Lentiviral manipulation of gene expression in human adult and embryonic stem cells
Clements, M.O., Godfrey, A., Crossley, J., Wilson, S.J., Takeuchi, Y. and Boshoff, C. 2006. Lentiviral manipulation of gene expression in human adult and embryonic stem cells. Tissue Engineering. 12 (7), pp. 1741-1751.
Mesenchymal stem cells: lineage, plasticity, and skeletal therapeutic potential
Oreffo, R.O.C., Cyrus, C., Mason, C. and Clements, M.O. 2005. Mesenchymal stem cells: lineage, plasticity, and skeletal therapeutic potential. Stem Cell Reviews. 1 (2), pp. 169-178.
Early molecular regulation of adipogenesis in human mesenchymal stem cells
Westwood, C., Henderson, S.R., Boshoff, C. and Clements, M.O. 2005. Early molecular regulation of adipogenesis in human mesenchymal stem cells. Keystone Symposium 2005: Molecular Regulation of Stem Cells. Banff, Canada 10-15 Feb 2005
Patterns of gene expression and novel factors involved in cartilage development identified through transcriptional analysis of hMSC chondrogenesis
Vujovic, S., Henderson, S.R., Clements, M.O. and Flanagan, A.M. 2005. Patterns of gene expression and novel factors involved in cartilage development identified through transcriptional analysis of hMSC chondrogenesis. Keystone Symposium 2005: Molecular Regulation of Stem Cells. Banff, Canada 10-15 Feb 2005
Book review: Human embryonic stem cells
Clements, M.O. 2004. Book review: Human embryonic stem cells. British Journal of Cancer. 90 (2), pp. 558-559. https://doi.org/10.1038/sj.bjc.6601577
The basis of persistent bacterial infections
Rhen, M., Eriksson, S., Clements, M.O., Bergstrom, S. and Normark, S.J. 2003. The basis of persistent bacterial infections. Trends in Microbiology. 11 (2), pp. 80-86. https://doi.org/10.1016/S0966-842X(02)00038-0
Germination of Bacillus cereus spores in response to L-alanine and to inosine: the roles of gerL and gerQ operons
Barlass, P.J., Houston, C.W., Clements, M.O. and Moir, A. 2002. Germination of Bacillus cereus spores in response to L-alanine and to inosine: the roles of gerL and gerQ operons. Microbiology. 148 (7), pp. 2089-2095.
Polynucleotide phosphorylase is a global regulator of virulence and persistency in Salmonella enterica
Clements, M.O., Eriksson, S., Thompson, A., Lucchini, S., Hinton, J.C.D., Normark, S.J. and Rhen, M. 2002. Polynucleotide phosphorylase is a global regulator of virulence and persistency in Salmonella enterica. Proceedings of the National Academy of Sciences of the United States of America. 99 (13), pp. 8784-8789. https://doi.org/10.1073/pnas.132047099
PerR controls oxidative stress resistance and iron storage proteins and is required for virulence in Staphylococcus aureus
Horsburgh, M.J., Clements, M.O., Crossley, H., Ingham, E. and Foster, S.J. 2001. PerR controls oxidative stress resistance and iron storage proteins and is required for virulence in Staphylococcus aureus. Infection and Immunity. 69 (6), pp. 3744-3754. https://doi.org/10.1128/IAI.69.6.3744-3754.2001
Virulence gene regulation in Salmonella enterica
Clements, M.O., Eriksson, S., Tezcan-Merdol, D., Hinton, J.C.D. and Rhen, M. 2001. Virulence gene regulation in Salmonella enterica. Annals of Medicine. 33 (3), pp. 178-185.
Cerebral proton and phosphorus-31 magnetic resonance spectroscopy in patients with subclinical hepatic encephalopathy
Taylor-Robinson, S.D., Buckley, C., Changani, K.K., Hodgson, H.J.F. and Bell, J.D. 1999. Cerebral proton and phosphorus-31 magnetic resonance spectroscopy in patients with subclinical hepatic encephalopathy. Liver. 19 (5), pp. 389-398. https://doi.org/10.1111/j.1478-3231.1999.tb00067.x
In vivo and in vitro hepatic phosphorus-31 magnetic resonance spectroscopy and electron microscopy in chronic ductopenic rejection of human liver allografts
Taylor-Robinson, S.D., Sargentoni, J., Bell, J.D., Thomas, E.L., Marcus, C.D., Changani, K.K., Saeed, N., Hodgson, H., Davidson, B.R., Burroughs, A.K., Rolles, K., Foster, C.S. and Cox, I.J. 1998. In vivo and in vitro hepatic phosphorus-31 magnetic resonance spectroscopy and electron microscopy in chronic ductopenic rejection of human liver allografts. Gut. 42 (5), pp. 735-43. https://doi.org/10.1136/gut.42.5.735
In vivo and in vitro hepatic 31P magnetic resonance spectroscopy and electron microscopy of the cirrhotic liver
Taylor-Robinson, S.D., Sargentoni, J., Bell, J.D., Saeed, N., Changani, K.K., Davidson, B.R., Rolles, K., Burroughs, A.K., Hodgson, H.J.F., Foster, C.S. and Cox, I.J. 1997. In vivo and in vitro hepatic 31P magnetic resonance spectroscopy and electron microscopy of the cirrhotic liver. Liver. 17 (4), pp. 198-209. https://doi.org/10.1111/j.1600-0676.1997.tb00806.x
Phosphorus-31 magnetic resonance spectroscopy of the human liver using chemical shift imaging techniques
Cox, I.J., Menon, D.K., Sargentoni, J., Bryant, D.J., Collins, A.G., Coutts, G.A., Iles, R.A., Bell, J.D., Benjamin, I.S., Gilbey, S., Hodgson, H.J.F. and Morgan, M.Y. 1992. Phosphorus-31 magnetic resonance spectroscopy of the human liver using chemical shift imaging techniques. Journal of Hepatology. 14 (2-3), pp. 265-275. https://doi.org/10.1016/0168-8278(92)90169-P
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