Clements, M.O., Eriksson, S., Thompson, A., Lucchini, S., Hinton, J.C.D., Normark, S.J. and Rhen, M. 2002. Polynucleotide phosphorylase is a global regulator of virulence and persistency in Salmonella enterica. Proceedings of the National Academy of Sciences of the United States of America. 99 (13), pp. 8784-8789.
| Title | Polynucleotide phosphorylase is a global regulator of virulence and persistency in Salmonella enterica |
|---|---|
| Authors | Clements, M.O., Eriksson, S., Thompson, A., Lucchini, S., Hinton, J.C.D., Normark, S.J. and Rhen, M. |
| Abstract | For many pathogens, the ability to regulate their replication in host cells is a key element in establishing persistency. Here, we identified a single point mutation in the gene for polynucleotide phosphorylase (PNPase) as a factor affecting bacterial invasion and intracellular replication, and which determines the alternation between acute or persistent infection in a mouse model for Salmonella enterica infection. In parallel, with microarray analysis, PNPase was found to affect the mRNA levels of a subset of virulence genes, in particular those contained in Salmonella pathogenicity islands 1 and 2. The results demonstrate a connection between PNPase and Salmonella virulence and show that alterations in PNPase activity could represent a strategy for the establishment of persistency. Copyright (2002) National Academy of Sciences, U.S.A. |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Journal citation | 99 (13), pp. 8784-8789 |
| ISSN | 0027-8424 |
| Year | 25 Jun 2002 |
| Digital Object Identifier (DOI) | doi:10.1073/pnas.132047099 |
| Web address (URL) | http://www.pnas.org/cgi/doi/10.1073/pnas.132047099 |
| Publication dates | |
| Published | 25 Jun 2002 |
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