Title | Campylobacter jejuni gene cj0511 encodes a serine peptidase essential for colonisation |
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Authors | Karlyshev, A.V., Thacker, G., Jones, M.A., Clements, M.O. and Wren, B.W. |
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Abstract | According to MEROPS peptidase database, Campylobacter species encode 64 predicted peptidases. However, proteolytic properties of only a few of these proteins have been confirmed experimentally. In this study we identified and characterised a Campylobacter jejuni gene cj0511 encoding a novel peptidase. The proteolytic activity associated with this enzyme was demonstrated in cell lysates. Moreover, enzymatic studies conducted with a purified protein confirmed a prediction of it being a serine peptidase. Furthermore, cj0511 mutant was found to be severely attenuated in chicken colonisation model, suggesting a role of the Cj0511 protein in infection. |
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Keywords | Campylobacter, protease, infection |
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Journal | FEBS Open Biology |
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Journal citation | 4, pp. 468-472 |
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ISSN | 2211-5463 |
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Year | 2014 |
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Publisher | Elsevier |
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Publisher's version | |
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Digital Object Identifier (DOI) | https://doi.org/10.1016/j.fob.2014.04.012 |
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Publication dates |
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Published | 09 May 2014 |
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