Expansion of CD4+ T cells with a cytotoxic phenotype in patients with B-chronic lymphocytic leukaemia (B-CLL)

Porakishvili, N., Roschupkina, T., Kalber, T., Jewell, A.P., Patterson, K., Yong, K.L. and Lydyard, P.M. 2001. Expansion of CD4+ T cells with a cytotoxic phenotype in patients with B-chronic lymphocytic leukaemia (B-CLL). Clinical and Experimental Immunology. 126 (1), pp. 29-36.

TitleExpansion of CD4+ T cells with a cytotoxic phenotype in patients with B-chronic lymphocytic leukaemia (B-CLL)
AuthorsPorakishvili, N., Roschupkina, T., Kalber, T., Jewell, A.P., Patterson, K., Yong, K.L. and Lydyard, P.M.
Abstract

Abnormal CD4/CD8 ratios and T-cell function have previously been shown in patients with B-chronic lymphocytic leukaemia (B-CLL). We have demonstrated that CD4+ T cells containing both serine esterase and perforin (PF) are increased in the blood of these patients. Using flow cytometry, we have shown that the CD4+ PF+ cells were CD57+ but lacked expression of CD28, suggesting a mature population. The same phenotype in CD8+ T cells is characteristic of mature cytotoxic T cells. However, in contrast to the CD8+ T cells, the CD4+ T cells were more frequently CD45RO positive than CD45RA positive, indicating prior antigen experience. In contrast, this population lacked expression of either CD69 or HLA-DR, arguing that they were not activated or that they are an abnormal population of T cells. Their constitutive cytokine levels showed them mainly to contain IL4 and not IFNgamma, suggesting a Th2 phenotype. The role of the CD4+ PF+ T-cell population is at present uncertain. However, this potentially cytotoxic T-cell population could contribute both to enhancing survival of the B-CLL tumour cells through production of IL4, and to the immunodeficient state frequently seen in patients with this tumour, independent of drug treatment.

JournalClinical and Experimental Immunology
Journal citation126 (1), pp. 29-36
ISSN0009-9104
YearOct 2001
Digital Object Identifier (DOI)doi:10.1046/j.1365-2249.2001.01639.x
Web address (URL)http://www.blackwell-synergy.com/doi/pdf/10.1046/j.1365-2249.2001.01639.x
Publication dates
PublishedOct 2001

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