Ultra-deep sequencing provides insights into the virology of hepatitis C super-infections in a case of three sequential infections with different genotypes

Chung, E., Ferns, R.B., He, M., Rigatti, R., Grant, P., McCormick, A.L., Bhagani, S., Webster, D.P., Nastouli, E. and Waters, L.J. 2015. Ultra-deep sequencing provides insights into the virology of hepatitis C super-infections in a case of three sequential infections with different genotypes. Journal of Clinical Virology. 70 (Sept), pp. 63-66. doi:10.1016/j.jcv.2015.06.105

TitleUltra-deep sequencing provides insights into the virology of hepatitis C super-infections in a case of three sequential infections with different genotypes
AuthorsChung, E., Ferns, R.B., He, M., Rigatti, R., Grant, P., McCormick, A.L., Bhagani, S., Webster, D.P., Nastouli, E. and Waters, L.J.
Abstract

The current epidemic of Hepatitis C infection in HIV-positive men who have sex with men is associated with increasing use of recreational drugs. Multiple HCV infections have been reported in haemophiliacs and intravenous drug users. Using ultra-deep sequencing analysis, we present the case of an HIV-positive MSM with evidence of three sequential HCV infections, each occurring during the acute phase of the preceding infection, following risk exposures. We observed rapid replacement of the original strain by the incoming genotype at subsequent time points. The impact of HCV super-infection remains unclear and UDS may provide new insights.

KeywordsHepatitis C; HIV; Ultra-deep sequencing; Super-infection; Genotype
JournalJournal of Clinical Virology
Journal citation70 (Sept), pp. 63-66
ISSN1386-6532
Year2015
PublisherElsevier
Digital Object Identifier (DOI)doi:10.1016/j.jcv.2015.06.105
Web address (URL)http://www.sciencedirect.com/science/article/pii/S1386653215002905
Publication dates
PublishedSep 2015

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