Fluorescence Characterization of Clinically-Important Bacteria

Dartnell, L.R., Roberts, T.A., Moore, G., Ward, J.M. and Muller, J-P. 2013. Fluorescence Characterization of Clinically-Important Bacteria. PLoS ONE. 8 (9) e75270. https://doi.org/10.1371/journal.pone.0075270

TitleFluorescence Characterization of Clinically-Important Bacteria
TypeJournal article
AuthorsDartnell, L.R., Roberts, T.A., Moore, G., Ward, J.M. and Muller, J-P.
Abstract

Healthcare-associated infections (HCAI/HAI) represent a substantial threat to patient health during hospitalization and incur billions of dollars additional cost for subsequent treatment. One promising method for the detection of bacterial contamination in a clinical setting before an HAI outbreak occurs is to exploit native fluorescence of cellular molecules for a hand-held, rapid-sweep surveillance instrument. Previous studies have shown fluorescence-based detection to be sensitive and effective for food-borne and environmental microorganisms, and even to be able to distinguish between cell types, but this powerful technique has not yet been deployed on the macroscale for the primary surveillance of contamination in healthcare facilities to prevent HAI. Here we report experimental data for the specification and design of such a fluorescence-based detection instrument. We have characterized the complete fluorescence response of eleven clinically-relevant bacteria by generating excitation-emission matrices (EEMs) over broad wavelength ranges. Furthermore, a number of surfaces and items of equipment commonly present on a ward, and potentially responsible for pathogen transfer, have been analyzed for potential issues of background fluorescence masking the signal from contaminant bacteria. These include bedside handrails, nurse call button, blood pressure cuff and ward computer keyboard, as well as disinfectant cleaning products and microfiber cloth. All examined bacterial strains exhibited a distinctive double-peak fluorescence feature associated with tryptophan with no other cellular fluorophore detected. Thus, this fluorescence survey found that an emission peak of 340nm, from an excitation source at 280nm, was the cellular fluorescence signal to target for detection of bacterial contamination. The majority of materials analysed offer a spectral window through which bacterial contamination could indeed be detected. A few instances were found of potential problems of background fluorescence masking that of bacteria, but in the case of the microfiber cleaning cloth, imaging techniques could morphologically distinguish between stray strands and bacterial contamination.

Article numbere75270
JournalPLoS ONE
Journal citation8 (9)
ISSN1932-6203
Year2013
PublisherPublic Library of Science
Publisher's version
Digital Object Identifier (DOI)https://doi.org/10.1371/journal.pone.0075270
Publication dates
Published30 Sep 2013

Related outputs

Research Trends and Future Perspectives in Marine Biomimicking Robotics
Aguzzi, J., Costa, C., Calisti, Marcello, Funari, Valerio, Stefanni, S., Danovaro, Roberto, Gomes, Helena I., Vecchi, Fabrizio, Dartnell, Lewis R., Weiss, Peter, Nowak, Kathrin, Chatzievangelou, D. and Marini, S. 2021. Research Trends and Future Perspectives in Marine Biomimicking Robotics. Sensors. 21 (11), p. e3778. https://doi.org/10.3390/s21113778

Do responses to the COVID-19 pandemic anticipate a long-lasting shift towards peer-to-peer production or degrowth?
Dartnell, L. and Kish, K. 2021. Do responses to the COVID-19 pandemic anticipate a long-lasting shift towards peer-to-peer production or degrowth? Sustainable Production and Consumption. 27, pp. 2165-2177. https://doi.org/10.1016/j.spc.2021.05.018

Infrared Spectroscopic Detection of Biosignatures at Lake Tírez, Spain: Implications for Mars
Preston, L.J., Barcenilla, R., Dartnell, L., Kucukkilic-Stephens, E. and Olsson-Francis, K. 2019. Infrared Spectroscopic Detection of Biosignatures at Lake Tírez, Spain: Implications for Mars. Astrobiology. 20 (1), pp. 15-25. https://doi.org/10.1089/ast.2019.2106

Origins: How The Earth Made Us
Dartnell, L.R. 2019. Origins: How The Earth Made Us. Bodley Head.

The paleo-environment reconstruction on Mars: focus points for the next astrobiology missions
Kereszturi, A., Kanuchova, Z., Dartnell, L. and Hauber, E. 2018. The paleo-environment reconstruction on Mars: focus points for the next astrobiology missions. in: Mehler, N. (ed.) Research Advances in Astronomy Nova Science Publishers. pp. 49-88

Society, Worldview and Outreach
Capova, K.A., Dartnell, L., Dunér, D., Melin, A. and Mitrikeski, P.T. 2018. Society, Worldview and Outreach. in: Capova, K.A., Persson, E., Milligan, T. and Dunér, D. (ed.) Astrobiology and Society in Europe Today Springer. pp. 19-24

Transitory Microbial Habitat in the Hyperarid Atacama Desert
Schulze-Makuch, D., Wagner, D., Kounaves, S.P., Mangelsdorf, K., Devine, K.G., de Vera, J-P., Schmitt-Kopplin, P., Grossart, H-P., Parro, V., Kaupenjohann, M., Galy, A., Schneider, B., Airo, A., Frösler, J., Davila, A.F., Arens, F.L., Cáceres, L., Cornejo, F.S., Carrizo, D., Dartnell, L.R., DiRuggiero, J., Flury, M., Ganzert, L., Gessner, M.O., Grathwohl, P., Guan, L., Heinz, J., Hess, M., Keppler, F., Maus, D., McKay, C.P., Meckenstock, R.U., Montgomery, W., Oberlin, E.A., Probst, A.J., Sáenz, J.S., Sattler, T., Schirmack, J., Sephton, M.A., Schloter, M., Uhl, J., Valenzuela, B., Vestergaard, G., Wörmer, L. and Zamorano, P. 2018. Transitory Microbial Habitat in the Hyperarid Atacama Desert. Proceedings of the National Academy of Sciences. 115 (11), pp. 2670-2675. https://doi.org/10.1073/pnas.1714341115

(Un)welcome Visitors: Why Aliens Might Visit Us
Dartnell, L.R. 2016. (Un)welcome Visitors: Why Aliens Might Visit Us. in: Al-Khalili, J. (ed.) Aliens: Science Asks: Is There Anyone Out There? Profile Books. pp. 25-34

The Astrobiology Primer v2.0
Domagal-Goldman, S.D., Wright, K.E., Adamala, K., de la Rubia Leigh, A., Bond, J., Dartnell, L.R., Goldman, A.D., Lynch, K., Naud, M.-E., Paulino-Lima, I.G., Kelsi, S., Walter-Antonio, M., Abrevaya, X.C., Anderson, R., Arney, G., Atri, D., Azúa-Bustos, A., Bowman, J.S., Brazelton, W.J., Brennecka, G.A., Carns, R., Chopra, A., Colangelo-Lillis, J., Crockett, C.J., DeMarines, J., Frank, E.A., Frantz, C., de la Fuente, E., Galante, D., Glass, J., Gleeson, D., Glein, C.R., Goldblatt, C., Horak, R., Horodyskyj, L., Kaçar, B., Kereszturi, A., Knowles, E., Mayeur, P., McGlynn, S., Miguel, Y., Montgomery, M., Neish, C., Noack, L., Rugheimer, S., Stüeken, E.E., Tamez-Hidalgo, P., Walker, S.I. and Wong, T. 2016. The Astrobiology Primer v2.0. Astrobiology. 16 (8), pp. 561-653. https://doi.org/10.1089/ast.2015.1460

Ionization of the Venusian atmosphere from solar and galactic cosmic rays
Nordheim, T.A., Dartnell, L., Desorgher, L., Coates, A.J. and Jones, G.H. 2015. Ionization of the Venusian atmosphere from solar and galactic cosmic rays. Icarus. 245, pp. 80-86. https://doi.org/10.1016/j.icarus.2014.09.032

Constraints on a potential aerial biosphere on Venus: I. Cosmic rays
Dartnell, L., Nordheim, T.A., Patel, M., Mason, J.P., Coates, A.J. and Jones, G.H. 2015. Constraints on a potential aerial biosphere on Venus: I. Cosmic rays. Icarus. 257, pp. 396-405. https://doi.org/10.1016/j.icarus.2015.05.006

Isolation of Radiation-Resistant Bacteria from Mars Analog Antarctic Dry Valleys by Preselection, and the Correlation between Radiation and Desiccation Resistance
Musilova, M., Wright, G., Ward, J.M. and Dartnell, L.R. 2015. Isolation of Radiation-Resistant Bacteria from Mars Analog Antarctic Dry Valleys by Preselection, and the Correlation between Radiation and Desiccation Resistance. Astrobiology. 15 (12), pp. 1076-1090. https://doi.org/10.1089/ast.2014.1278

Planetary habitability: lessons learned from terrestrial analogues
Preston, L.J. and Dartnell, L. 2014. Planetary habitability: lessons learned from terrestrial analogues. International Journal of Astrobiology. 13 (1). https://doi.org/10.1017/S1473550413000396

Degradation of microbial fluorescence biosignatures by solar ultraviolet radiation on Mars
Dartnell, L. and Patel, M.R. 2014. Degradation of microbial fluorescence biosignatures by solar ultraviolet radiation on Mars. International Journal of Astrobiology. 13 (2), pp. 112-123. https://doi.org/10.1017/S1473550413000335

An Origin-of-Life Reactor to Simulate Alkaline Hydrothermal Vents
Herschy, B., Whicher, A., Camprubi, E., Watson, C., Dartnell, L., Ward, J., Evans, J.R.G. and Lane, N. 2014. An Origin-of-Life Reactor to Simulate Alkaline Hydrothermal Vents. Journal of Molecular Evolution. 79 (5-6), pp. 213-227. https://doi.org/10.1007/s00239-014-9658-4

Fluorescent analysis of photosynthetic microbes and Polycyclic Aromatic Hydrocarbons linked to optical remote sensing
Zhang, D., Muller, J.-P., Lavender, S., Walton, D. and Dartnell, L. 2012. Fluorescent analysis of photosynthetic microbes and Polycyclic Aromatic Hydrocarbons linked to optical remote sensing. International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences. XXXIX-B8, pp. 555-559. https://doi.org/10.5194/isprsarchives-XXXIX-B8-555-2012

Martian sub-surface ionising radiation: biosignatures and geology
Dartnell, L., Desorgher, L., Ward, J.M. and Coates, A.J. 2007. Martian sub-surface ionising radiation: biosignatures and geology. Biogeosciences. 4, pp. 545-558.

Permalink - https://westminsterresearch.westminster.ac.uk/item/qw580/fluorescence-characterization-of-clinically-important-bacteria


Share this
Tweet
Email

Usage statistics

37 total views
10 total downloads
1 views this month
1 downloads this month
These values are for the period from September 2nd 2018, when this repository was created

Export as