Fluorescent analysis of photosynthetic microbes and Polycyclic Aromatic Hydrocarbons linked to optical remote sensing

Zhang, D., Muller, J.-P., Lavender, S., Walton, D. and Dartnell, L. 2012. Fluorescent analysis of photosynthetic microbes and Polycyclic Aromatic Hydrocarbons linked to optical remote sensing. International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences. XXXIX-B8, pp. 555-559. doi:10.5194/isprsarchives-XXXIX-B8-555-2012

TitleFluorescent analysis of photosynthetic microbes and Polycyclic Aromatic Hydrocarbons linked to optical remote sensing
TypeJournal article
AuthorsZhang, D., Muller, J.-P., Lavender, S., Walton, D. and Dartnell, L.
Abstract

Fluorescence analysis, being a non-invasive technique, has become one of the most powerful and widely used techniques for microbiologists and chemists to study various types of sample from photosynthetic microbes to hydrocarbons. The work reported here focuses on experimental results of fluorescent features of photosynthetic microbial species (cyanobacteria) and also five different crude oil samples. The cyanobacteria samples were collected from the Baltic Sea at the end of July 2011 and were associated with cyanobacterial bloom events, and the crude oil samples were from various oil spill events. The aim of the study was to find fluorescent biosignatures of cyanobacteria (initially a species specific to the Baltic Sea) and the fingerprints of crude oil; oil spills can be difficult to differentiate from biogenic films when using Synthetic Aperture Radar (SAR) or sunglint contaminated optical imagery. All samples were measured using a Perkin Elmer LS55 Luminescence spectrometer over a broad range of excitation and emission wavelength from ultraviolet (UV) to near infrared (NIR). The results are presented in Excitation Emission Matrices (EEMs) that exhibit the fluorescent features of each sample. In the EEM of the seawater sample containing cyanobacteria, there is an intense emission peak from tryptophan with fluorescent excitation and emission peaks at 285 and 345 nm respectively. In addition, fluorescent signatures of phycocyanin and chlorophyll-a are present with excitation and emission centre wavelengths at 555 nm, 645 nm and 390 nm, 685 nm, respectively. Additionally, the fluorescence signatures of Polycyclic Aromatic Hydrocarbons (PAHs) are present in the EEMs of crude oil samples with excitation and emission peaks at 285 nm and 425 nm. This study underpins further research on how to distinguish cyanobacteria species by their fluorescence signatures and the potential role that PAHs play in detection of cyanobacteria fluorescence features.

KeywordsFluorescence
Excitation-Emission Matrix
Cyanobacteria
Crude oil
PAHs
JournalInternational Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences
Journal citationXXXIX-B8, pp. 555-559
ISSN1682-1750
Year2012
PublisherCopernicus Publications
Publisher's versionisprsarchives-XXXIX-B8-555-2012.pdf
Digital Object Identifier (DOI)doi:10.5194/isprsarchives-XXXIX-B8-555-2012
LicenseCC BY 3.0

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