The Role of Differentially Expressed miRNAs and Potential miRNA-mRNA Regulatory Network in Prostate Cancer Progression and Metastasis

Arisan, E.D., Freitas, I.L., Pazoki, K., Rahim, S. and Uysal Onganer, P. 2020. The Role of Differentially Expressed miRNAs and Potential miRNA-mRNA Regulatory Network in Prostate Cancer Progression and Metastasis. Biomedical Journal of Technical and Scientific Research. 25 (4), pp. 19382-19387. https://doi.org/10.26717/BJSTR.2020.25.004241

TitleThe Role of Differentially Expressed miRNAs and Potential miRNA-mRNA Regulatory Network in Prostate Cancer Progression and Metastasis
TypeJournal article
AuthorsArisan, E.D., Freitas, I.L., Pazoki, K., Rahim, S. and Uysal Onganer, P.
Abstract

Purpose: Aberrant expression of microRNAs (miRNAs) has been discovered in prostate cancer progression however their function is not well understood, thereby further investigation is required to understand the importance of underlying mechanisms and their involvement in multiple signaling pathways, as well as their potential as therapeutic targets. In this study the role and expression levels of three miRNAs were evaluated: miR-21, miR-221 and miR-200c in different prostate cancer cell lines. In addition, based on the latest studies on miRNAs function, their association with other target genes and molecules were analyzed using bioinformatic tools.
Methods: Three PCa cell lines PC3, LNCaP and VCaP and normal prostate epithelial cell line PNT1A were screened for miRNA expression levels using qPCR. miRNA target genes and their association with signaling pathways were analyzed through several Network and pathway analysis online tools.
Findings: Upregulation of miR-21 and miR-221 was observed in PC3 and VCaP prostate cancer cells, respectively. According to KEGG analysis, we found that Hippo signaling pathway and cytokine-cytokine receptor interactions were affected by miR-21 while miR-221 would interfere with ECM-receptor interaction, Fatty acid elongation and Huntington disease molecular networks. Exposure of PC3 cells to TGF-β (10 µM) caused upregulation of miR-21 with the evidence with increased invasion potential.
Discussion and Conclusion: miRNAs could regulate several genes in multiple signaling pathways. Here, we demonstrated that in a panel of PCa cell lines, both mir-21 and miR-221 expressions were upregulated. miR-21 may be a dignostic and prognosticbiomarker for PCa.

JournalBiomedical Journal of Technical and Scientific Research
Journal citation25 (4), pp. 19382-19387
ISSN2574-1241
Year2020
PublisherBiomedical Journal of Scientific and Technical Research
Publisher's version
License
CC BY 4.0
File Access Level
Open (open metadata and files)
Digital Object Identifier (DOI)https://doi.org/10.26717/BJSTR.2020.25.004241
Web address (URL)https://biomedres.us/pdfs/BJSTR.MS.ID.004241.pdf
Publication dates
Published18 Feb 2020

Related outputs

microRNA 1307 Is a Potential Target for SARS-CoV-2 Infection: An <i>in Vitro</i> Model
Arisan, Elif Damla, Dart, D. Alwyn, Grant, Guy H., Dalby, A.R., Kancagi, Derya Dilek, Turan, Raife Dilek, Yurtsever, Bulut, Karakus, Gozde Sir, Ovali, Ercument, Lange, Sigrun and Uysal-Onganer, P. 2022. microRNA 1307 Is a Potential Target for SARS-CoV-2 Infection: An <i>in Vitro</i> Model. ACS Omega. 7 (42), pp. 38003-38014. https://doi.org/10.1021/acsomega.2c05245

3D Disease Modelling of Hard and Soft Cancer Using PHA-Based Scaffolds
Tomar, A., Uysal-Onganer, P., Basnett, P., Pati, U. and Roy, I. 2022. 3D Disease Modelling of Hard and Soft Cancer Using PHA-Based Scaffolds. Cancers. 14 (14) e3549. https://doi.org/10.3390/cancers14143549

microRNA expression in acute myeloid leukaemia: New targets for therapy?
Fletcher, D., Brown, E., Javadala, J., Uysal Onganer, P. and Guinn, B-A. 2022. microRNA expression in acute myeloid leukaemia: New targets for therapy? European Journal of Haemotology. 3 (3), pp. 596-608. https://doi.org/10.1002/jha2.441

Epigenetic Mechanisms of Metal Carcinogenicity
Manić, L., Wallace, D., Uysal Onganer, P., Taalab, Y.M., Farooqi, A.A., Antonijević, B. and Djordjevic, A.B. 2022. Epigenetic Mechanisms of Metal Carcinogenicity. Toxicology Reports. 9, pp. 778-787. https://doi.org/10.1016/j.toxrep.2022.03.037

Nickel's Role in Pancreatic Ductal Adenocarcinoma: Potential Involvement of microRNAs
Mortoglou, M., Manić, L., Buha Djordjevic, A., Bulat, Z., Đorđević, Vladimir, Manis, Katherine, Valle, Elizabeth, York, Lauren, Wallace, D. and Uysal Onganer, P. 2022. Nickel's Role in Pancreatic Ductal Adenocarcinoma: Potential Involvement of microRNAs. Toxics. 10 (3) 148. https://doi.org/10.3390/toxics10030148

microRNA-21 Regulates Stemness in Pancreatic Ductal Adenocarcinoma Cells
Mortoglou, M., Miralles, F., Arisan, E.D., Dart, A., Jurcevic, S., Lange, S. and Uysal Onganer, P. 2022. microRNA-21 Regulates Stemness in Pancreatic Ductal Adenocarcinoma Cells. International Journal of Molecular Sciences. 23 (3) 1275. https://doi.org/10.3390/ijms23031275

Role of microRNAs in response to cadmium chloride in pancreatic ductal adenocarcinoma
Mortoglou, M., Djordjevic, A.B., Djordjevic, V., Collins, H., York, L., Mani, K., Valle, E., Wallace, D. and Uysal Onganer, P. 2021. Role of microRNAs in response to cadmium chloride in pancreatic ductal adenocarcinoma. Archives of Toxicology. 96, p. 467–485. https://doi.org/10.1007/s00204-021-03196-9

The Role of CDK4 in the Pathogenesis of Pancreatic Cancer.
Jiggens, E., Mortoglou, M., Grant, G.H. and Uysal-Onganer, P. 2021. The Role of CDK4 in the Pathogenesis of Pancreatic Cancer. Healthcare. 9 (11) 1478. https://doi.org/10.3390/healthcare9111478

In Vitro Investigations of miR-33a Expression in Estrogen Receptor-Targeting Therapies in Breast Cancer Cells
Ozfiliz Kilbas, P., Ozlem Sonmez, Pinar Obakan-Yerlikaya, Ajda Coker-Gurkan, Narcin Palavan-Ünsal, Uysal Onganer, P. and Arisan, E. 2021. In Vitro Investigations of miR-33a Expression in Estrogen Receptor-Targeting Therapies in Breast Cancer Cells. Cancers. 13 (21), p. e5322. https://doi.org/10.3390/cancers13215322

Prostate Cancer Cell Extracellular Vesicles Increase Mineralisation of Bone Osteoblast Precursor Cells in an In Vitro Model
Lanning, Ben, Webber, J., Uysal Onganer, P., Jiang, W., Clayton, Aled and Dart, D. 2021. Prostate Cancer Cell Extracellular Vesicles Increase Mineralisation of Bone Osteoblast Precursor Cells in an In Vitro Model. Biology. 10 (4), p. e318. https://doi.org/10.3390/biology10040318

Non-coding RNAs in pancreatic ductal adenocarcinoma: New approaches for better diagnosis and therapy
Mortoglou, M., Tabin, Z.K., Arisan, E.D., Kocher, H.M. and Uysal Onganer, P. 2021. Non-coding RNAs in pancreatic ductal adenocarcinoma: New approaches for better diagnosis and therapy. Translational Oncology. 14 (7) 101090. https://doi.org/10.1016/j.tranon.2021.101090

AMPK Is the Crucial Target for the CDK4/6 Inhibitors Mediated Therapeutic Responses in PANC-1 and MIA PaCa-2 Pancreatic Cancer Cell Lines
Sevgin, B., Coban, M., Özge Rencuzogullari, Coker Gurkan, A., Obakan Yerlikaya, P., Uysal Onganer, P. and Arisan, E. 2021. AMPK Is the Crucial Target for the CDK4/6 Inhibitors Mediated Therapeutic Responses in PANC-1 and MIA PaCa-2 Pancreatic Cancer Cell Lines. Stresses. 1 (1), pp. 48-68. https://doi.org/10.3390/stresses1010005

MicroRNA-Regulated Signaling Pathways: Potential Biomarkers for Pancreatic Ductal Adenocarcinoma
Mortoglou, M., Wallace, D., Buha Djordjevic, A., Vladimir Djordjevic, Arisan, E. and Uysal Onganer, P. 2021. MicroRNA-Regulated Signaling Pathways: Potential Biomarkers for Pancreatic Ductal Adenocarcinoma. Stresses. 1 (1), pp. 30-47. https://doi.org/10.3390/stresses1010004

MicroRNAs for Virus Pathogenicity and Host Responses, Identified in SARS-CoV-2 Genomes, May Play Roles in Viral-Host Co-Evolution in Putative Zoonotic Host Species
Lange, S., Arisan, E., Grant, G.H. and Uysal Onganer, P. 2021. MicroRNAs for Virus Pathogenicity and Host Responses, Identified in SARS-CoV-2 Genomes, May Play Roles in Viral-Host Co-Evolution in Putative Zoonotic Host Species. Viruses. 13 (1) 117. https://doi.org/10.3390/v13010117

MiR-21 Is Required for the Epithelial–Mesenchymal Transition in MDA-MB-231 Breast Cancer Cells
Arisan, E., Rencuzogullari, O., Clara Cieza-Borrella, Miralles Arenas, F., Miriam Dwek, Lange, S. and Uysal Onganer, P. 2021. MiR-21 Is Required for the Epithelial–Mesenchymal Transition in MDA-MB-231 Breast Cancer Cells. International Journal of Molecular Sciences. 22 (4), p. e1557 1557. https://doi.org/10.3390/ijms22041557

Peptidylarginine Deiminase Inhibitor Application, Using Cl-Amidine, PAD2, PAD3 and PAD4 Isozyme-Specific Inhibitors in Pancreatic Cancer Cells, Reveals Roles for PAD2 and PAD3 in Cancer Invasion and Modulation of Extracellular Vesicle Signatures
Uysal Onganer, P., D'alessio, S., Mortoglou, M., Kraev, I. and Lange, S. 2021. Peptidylarginine Deiminase Inhibitor Application, Using Cl-Amidine, PAD2, PAD3 and PAD4 Isozyme-Specific Inhibitors in Pancreatic Cancer Cells, Reveals Roles for PAD2 and PAD3 in Cancer Invasion and Modulation of Extracellular Vesicle Signatures. International Journal of Molecular Sciences. 22 (3) 1396. https://doi.org/10.3390/ijms22031396

MicroRNAs for Virus Pathogenicity and Host Responses, Identified in SARS-CoV-2 Genomes, May Play Roles in Viral-Host Co-Evolution in Putative Zoonotic Host Species
Lange, S., Arisan, E.D., Grant, G.H. and Uysal Onganer, P. 2021. MicroRNAs for Virus Pathogenicity and Host Responses, Identified in SARS-CoV-2 Genomes, May Play Roles in Viral-Host Co-Evolution in Putative Zoonotic Host Species. MDPI Viruses. 13 (1), p. 117 10.3390/v13010117 . https://doi.org/10.3390/v13010117

Elevated microRNA expression could be diagnostic biomarker for PDAC
Mortoglou, M., Arisan, D., Ferreira, T., McCormick, A.L. and Uysal Onganer, P. 2020. Elevated microRNA expression could be diagnostic biomarker for PDAC. Pancreatology. 20 (8) e19. https://doi.org/10.1016/j.pan.2020.10.015

Specific c-Jun N-Terminal Kinase Inhibitor, JNK-IN-8 Suppresses Mesenchymal Profile of PTX-Resistant MCF-7 Cells through Modulating PI3K/Akt, MAPK and Wnt Signaling Pathways
Ozfiliz Kilbas, P., Sonmez, Ozlem, Uysal Onganer, P., Coker Gurkan, A., Obakan Yerlikaya, P. and Arisan, Elif Damla 2020. Specific c-Jun N-Terminal Kinase Inhibitor, JNK-IN-8 Suppresses Mesenchymal Profile of PTX-Resistant MCF-7 Cells through Modulating PI3K/Akt, MAPK and Wnt Signaling Pathways. Biology. 9 (10), p. e320. https://doi.org/10.3390/biology9100320

Putative Roles for Peptidylarginine Deiminases in COVID-19
Arisan, E., Uysal Onganer, P. and Lange, S. 2020. Putative Roles for Peptidylarginine Deiminases in COVID-19. International Journal of Molecular Sciences. 21 (13) 4662. https://doi.org/10.3390/ijms21134662

The Prediction of miRNAs in SARS-CoV-2 Genomes: hsa-miR Databases Identify 7 Key miRs Linked to Host Responses and Virus Pathogenicity-Related KEGG Pathways Significant for Comorbidities
Arisan, E.D., Dart, A., Grant, G.H., Arisan, S., Cuhadaroglu, S., Lange, S. and Uysal Onganer, P. 2020. The Prediction of miRNAs in SARS-CoV-2 Genomes: hsa-miR Databases Identify 7 Key miRs Linked to Host Responses and Virus Pathogenicity-Related KEGG Pathways Significant for Comorbidities. MDPI Viruses. 12 (6) 614. https://doi.org/10.3390/v12060614

Protein Deimination Signatures in Plasma and Plasma-EVs and Protein Deimination in the Brain Vasculature in a Rat Model of Pre-Motor Parkinson’s Disease
Marco Sancandi, Uysal Onganer, P., Igor Kraev, Mercer, A. and Lange, S. 2020. Protein Deimination Signatures in Plasma and Plasma-EVs and Protein Deimination in the Brain Vasculature in a Rat Model of Pre-Motor Parkinson’s Disease. International Journal of Molecular Sciences. 21 (8) 2743. https://doi.org/10.3390/ijms21082743

Demonstration of microRNA using in situ hybridisation on formalin fixed paraffin wax samples using conventional oligonucleotide probes: a comparison with the use of locked nucleic acid probes
Warford, A., Rahman, N.S., Ribeiro, D.A. and Uysal Onganer, P. 2020. Demonstration of microRNA using in situ hybridisation on formalin fixed paraffin wax samples using conventional oligonucleotide probes: a comparison with the use of locked nucleic acid probes. British Journal of Biomedical Science. 77 (3), pp. 135-141. https://doi.org/10.1080/09674845.2020.1748913

Upregulated wnt-11 and mir-21 expression trigger epithelial mesenchymal transition in aggressive prostate cancer cells
Arisan, E.D., Rencuzogullari, O., Freitas, I.L., Radzali, S., Keskin, B., Kothari, A., Warford, A. and Uysal-Onganer, P. 2020. Upregulated wnt-11 and mir-21 expression trigger epithelial mesenchymal transition in aggressive prostate cancer cells. Biology. 9 (3) 52. https://doi.org/10.3390/biology9030052

Deiminated Proteins and Extracellular Vesicles as Novel Biomarkers in Pinnipeds: Grey seal (Halichoerus gryptus) and Harbour seal (Phoca vitulina)
Magnadóttir, B., Uysal Onganer, P., Kraev, I., Svansson, V., Skírnisson, K. and Lange, S. 2020. Deiminated Proteins and Extracellular Vesicles as Novel Biomarkers in Pinnipeds: Grey seal (Halichoerus gryptus) and Harbour seal (Phoca vitulina). Biochimie . 171-172, pp. 79-90. https://doi.org/10.1016/j.biochi.2020.02.017

Peptidylarginine Deiminase Isozyme-Specific PAD2, PAD3 and PAD4 Inhibitors Differentially Modulate Extracellular Vesicle Signatures and Cell Invasion in Two Glioblastoma Multiforme Cell Lines
Uysal Onganer, P., Amy MacLatchy, Rayan Mahmoud, Kraev, I., Paul R. Thompson, Jameel M. Inal and Lange, S. 2020. Peptidylarginine Deiminase Isozyme-Specific PAD2, PAD3 and PAD4 Inhibitors Differentially Modulate Extracellular Vesicle Signatures and Cell Invasion in Two Glioblastoma Multiforme Cell Lines. International Journal of Molecular Sciences. 21 (4) 1495. https://doi.org/10.3390/ijms21041495

Deiminated proteins and extracellular vesicles - Novel serum biomarkers in whales and orca
Magnadóttir, B., Uysal-Onganer, P., Kraev, I., Svansson, V., Hayes, P.M. and Lange, S. 2020. Deiminated proteins and extracellular vesicles - Novel serum biomarkers in whales and orca. Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics. 34, p. 100676 100676. https://doi.org/10.1016/j.cbd.2020.100676

Extracellular Vesicles, Deiminated Protein Cargo and microRNAs are Novel Serum Biomarkers for Environmental Rearing Temperature in Atlantic Cod (Gadus morhua L.)
Magnadóttir, B., Uysal Onganer, P., Kraev, I., Dodds, A.W., Gudmundsdottir, S. and Lange, S. 2020. Extracellular Vesicles, Deiminated Protein Cargo and microRNAs are Novel Serum Biomarkers for Environmental Rearing Temperature in Atlantic Cod (Gadus morhua L.) . Aquaculture Reports. 16 100245. https://doi.org/10.1016/j.aqrep.2019.100245

Wnt-11 expression promotes invasiveness and correlates with survival in human pancreatic ductal adeno carcinoma
Dart, D.A., Arisan, D.E., Owen, S., Hao, C., Jiang, W.G. and Uysal-Onganer, P. 2019. Wnt-11 expression promotes invasiveness and correlates with survival in human pancreatic ductal adeno carcinoma. Genes. 10 (11) 921. https://doi.org/10.3390/genes10110921

Post-Translational Deimination of Immunological and Metabolic Protein Markers in Plasma and Extracellular Vesicles of Naked Mole-Rat (Heterocephalus glaber)
Matthew E. Pamenter, Uysal Onganer, P., Kenny W. Huynh, Kraev, I. and Lange, S. 2019. Post-Translational Deimination of Immunological and Metabolic Protein Markers in Plasma and Extracellular Vesicles of Naked Mole-Rat (Heterocephalus glaber). International Journal of Molecular Sciences. 20 (21) 5378. https://doi.org/10.3390/ijms20215378

Peptidylarginine Deiminases Post-translationally deiminate Prohibitin and modulate Extracellular Vesicle Release and microRNAs in Glioblastoma Multiforme
Kosgodage, U., Uysal Onganer, P., Maclatchy, A., Kraev, I., Chatterton, N.P., Nicholas, A.P., Inal, J.M. and Lange, S. 2019. Peptidylarginine Deiminases Post-translationally deiminate Prohibitin and modulate Extracellular Vesicle Release and microRNAs in Glioblastoma Multiforme . International Journal of Molecular Sciences. 20 (1) 103. https://doi.org/10.3390/ijms20010103

Cannabidiol Affects Extracellular Vesicle Release, miR21 and miR126, and Reduces Prohibitin Protein in Glioblastoma Multiforme Cells
Kosgodage, U., Uysal Onganer, P., Maclatchy, A., Mould, R., Nunn, A.V.W., Guy, G.W., Kraev, I., Chatterton, N.P., Thomas, E.L., Inal, J.M., Bell, J.D. and Lange, S. 2019. Cannabidiol Affects Extracellular Vesicle Release, miR21 and miR126, and Reduces Prohibitin Protein in Glioblastoma Multiforme Cells. Translational Oncology. 12 (3), pp. 513-522. https://doi.org/10.1016/j.tranon.2018.12.004

Prostate-specific PTen deletion in mice activates inflammatory microRNA expression pathways in the epithelium early in hyperplasia development
Dart Alwyn, D., Uysal-Onganer, P. and Jiang, W.G. 2017. Prostate-specific PTen deletion in mice activates inflammatory microRNA expression pathways in the epithelium early in hyperplasia development. Oncogenesis. 14 (6) 400. https://doi.org/10.1038/s41389-017-0007-5

The interaction of Wnt-11 and signalling cascades in prostate cancer
Koushyar, S., Grant, G.H. and Uysal-Onganer, P. 2016. The interaction of Wnt-11 and signalling cascades in prostate cancer. Tumor Biology. 37 (10), pp. 13049-13057. https://doi.org/10.1007/s13277-016-5263-z

Distinct expression and activity of GSK-3α and GSK-3β in prostate cancer
Darrington, R.S., Campa, V.M., Walker, M.M., Bengoa-Vergniory, N., Gorrono-Etxebarria, I., Uysal-Onganer, P., Kawano, Y., Waxman, J. and Kypta, R.M. 2012. Distinct expression and activity of GSK-3α and GSK-3β in prostate cancer. International Journal of Cancer. 131 (6), p. E872–E883. https://doi.org/10.1002/ijc.27620

Secreted Frizzled-related protein-1 is a negative regulator of androgen receptor activity in prostate cancer
Kawano, Y., Diez, S., Uysal-Onganer, P., Darrington, R.S., Waxman, J. and Kypta, R.M. 2009. Secreted Frizzled-related protein-1 is a negative regulator of androgen receptor activity in prostate cancer. British Journal of Cancer. 100, pp. 1165-1174. https://doi.org/10.1038/sj.bjc.6604976

Epidermal growth factor upregulates motility of Mat-LyLu rat prostate cancer cells partially via voltage-gated Na+ channel activity
Ding, Y., Brackenbury, W.J., Uysal Onganer, P., Montano, X., Porter, L.M., Bates, L.F. and Djamgoz, M.B.A. 2008. Epidermal growth factor upregulates motility of Mat-LyLu rat prostate cancer cells partially via voltage-gated Na+ channel activity. Journal of Cellular Physiology. 215 (1), pp. 77-81. https://doi.org/10.1002/jcp.21289

The expression and functional characterization of sigma (sigma) 1 receptors in breast cancer cell lines
Aydar, E., Uysal Onganer, P., Perrett, R., Djamgoz, M.B.A. and Palmer, C.P. 2006. The expression and functional characterization of sigma (sigma) 1 receptors in breast cancer cell lines. Cancer Letters. 242 (2), pp. 245-257. https://doi.org/10.1016/j.canlet.2005.11.011

An acetylcholinesterase-derived peptide inhibits endocytic membrane activity in a human metastatic breast cancer cell line
Uysal Onganer, P., Djamgoz, M.B.A., Whyte, K. and Greenfield, S.A. 2006. An acetylcholinesterase-derived peptide inhibits endocytic membrane activity in a human metastatic breast cancer cell line. Biochimica et Biophysica Acta: general subjects. 1760 (3), pp. 415-420. https://doi.org/10.1016/j.bbagen.2005.12.016

Small-cell lung cancer (human): potentiation of endocytic membrane activity by voltage-gated Na(+) channel expression in vitro
Uysal Onganer, P. and Djamgoz, M.B.A. 2005. Small-cell lung cancer (human): potentiation of endocytic membrane activity by voltage-gated Na(+) channel expression in vitro. Journal of Membrane Biology. 204, pp. 67-75. https://doi.org/10.1007/s00232-005-0747-6

Neuronal characteristics of small-cell lung cancer
Uysal Onganer, P., Seckl, M.J. and Djamgoz, M.B.A. 2005. Neuronal characteristics of small-cell lung cancer. British Journal of Cancer. 93, pp. 1197-1201. https://doi.org/10.1038/sj.bjc.6602857

Permalink - https://westminsterresearch.westminster.ac.uk/item/qyq7x/the-role-of-differentially-expressed-mirnas-and-potential-mirna-mrna-regulatory-network-in-prostate-cancer-progression-and-metastasis


Share this

Usage statistics

117 total views
46 total downloads
These values cover views and downloads from WestminsterResearch and are for the period from September 2nd 2018, when this repository was created.