Role of microRNAs in response to cadmium chloride in pancreatic ductal adenocarcinoma

Mortoglou, M., Djordjevic, A.B., Djordjevic, V., Collins, H., York, L., Mani, K., Valle, E., Wallace, D. and Uysal Onganer, P. 2021. Role of microRNAs in response to cadmium chloride in pancreatic ductal adenocarcinoma. Archives of Toxicology. 96, p. 467–485. https://doi.org/10.1007/s00204-021-03196-9

TitleRole of microRNAs in response to cadmium chloride in pancreatic ductal adenocarcinoma
TypeJournal article
AuthorsMortoglou, M., Djordjevic, A.B., Djordjevic, V., Collins, H., York, L., Mani, K., Valle, E., Wallace, D. and Uysal Onganer, P.
Abstract

Pancreatic ductal adenocarcinoma (PDAC) is one of the most fatal and aggressive malignancies with a 5-year survival rate less than 9%. Early detection is particularly difficult due to the lack of symptoms even in advanced stages. microRNAs (miRs/miRNAs) are small (~18–24 nucleotides), endogenous, non-coding RNAs, which are involved in the pathogenesis of several malignancies including PDAC. Alterations of miR expressions can lead to apoptosis, angiogenesis, and metastasis.
The role of environmental pollutants such as cadmium (Cd) in PDAC has been suggested but not fully understood. This study underlines the role of miRs (miR-221, miR-155, miR-126) in response to cadmium chloride (CdCl2) in vitro. Lethal concentration (LC50) values for CdCl2 resulted in a toxicity series of AsPC-1>HPNE>BxPC3>Panc-1=Panc-10.5. Following the treatment with CdCl2, miR-221 and miR-155 were significantly overexpressed, whereas miR-126 was downregulated.
An increase in epithelial–mesenchymal transition (EMT) via the dysregulation of mesenchymal markers such as Wnt-11,E-cadherin, Snail, and Zeb1 was also observed. Hence, this study has provided evidence to suggest that the environmental pollutant Cd can have a signifcant role in the development of PDAC, suggesting a signifcant correlation between miRs and Cd exposure during PDAC progression. Further studies are needed to investigate the precise role of miRs in PDAC progression as well as the role of Cd and other environmental pollutants.

JournalArchives of Toxicology
Journal citation96, p. 467–485
ISSN1432-0738
0340-5761
Year2021
PublisherSpringer
Publisher's version
License
CC BY 4.0
File Access Level
Open (open metadata and files)
Digital Object Identifier (DOI)https://doi.org/10.1007/s00204-021-03196-9
Publication dates
Published online14 Dec 2021
Published in print2022

Related outputs

microRNA expression in acute myeloid leukaemia: New targets for therapy?
Fletcher, D., Brown, E., Javadala, J., Uysal Onganer, P. and Guinn, B-A. 2022. microRNA expression in acute myeloid leukaemia: New targets for therapy? European Journal of Haemotology. Advanced online publication. https://doi.org/10.1002/jha2.441

Epigenetic Mechanisms of Metal Carcinogenicity
Manić, L., Wallace, D., Uysal Onganer, P., Taalab, Y.M., Farooqi, A.A., Antonijević, B. and Djordjevic, A.B. 2022. Epigenetic Mechanisms of Metal Carcinogenicity. Toxicology Reports. 9, pp. 778-787. https://doi.org/10.1016/j.toxrep.2022.03.037

Nickel's Role in Pancreatic Ductal Adenocarcinoma: Potential Involvement of microRNAs
Mortoglou, M., Manić, L., Buha Djordjevic, A., Bulat, Z., Đorđević, Vladimir, Manis, Katherine, Valle, Elizabeth, York, Lauren, Wallace, D. and Uysal Onganer, P. 2022. Nickel's Role in Pancreatic Ductal Adenocarcinoma: Potential Involvement of microRNAs. Toxics. 10 (3) 148. https://doi.org/10.3390/toxics10030148

microRNA-21 Regulates Stemness in Pancreatic Ductal Adenocarcinoma Cells
Mortoglou, M., Miralles, F., Arisan, E.D., Dart, A., Jurcevic, S., Lange, S. and Uysal Onganer, P. 2022. microRNA-21 Regulates Stemness in Pancreatic Ductal Adenocarcinoma Cells. International Journal of Molecular Sciences. 23 (3) 1275. https://doi.org/10.3390/ijms23031275

The Role of CDK4 in the Pathogenesis of Pancreatic Cancer.
Jiggens, E., Mortoglou, M., Grant, G.H. and Uysal-Onganer, P. 2021. The Role of CDK4 in the Pathogenesis of Pancreatic Cancer. Healthcare. 9 (11) 1478. https://doi.org/10.3390/healthcare9111478

In Vitro Investigations of miR-33a Expression in Estrogen Receptor-Targeting Therapies in Breast Cancer Cells
Ozfiliz Kilbas, P., Ozlem Sonmez, Pinar Obakan-Yerlikaya, Ajda Coker-Gurkan, Narcin Palavan-Ünsal, Uysal Onganer, P. and Arisan, E. 2021. In Vitro Investigations of miR-33a Expression in Estrogen Receptor-Targeting Therapies in Breast Cancer Cells. Cancers. 13 (21), p. e5322. https://doi.org/10.3390/cancers13215322

Prostate Cancer Cell Extracellular Vesicles Increase Mineralisation of Bone Osteoblast Precursor Cells in an In Vitro Model
Lanning, Ben, Webber, J., Uysal Onganer, P., Jiang, W., Clayton, Aled and Dart, D. 2021. Prostate Cancer Cell Extracellular Vesicles Increase Mineralisation of Bone Osteoblast Precursor Cells in an In Vitro Model. Biology. 10 (4), p. e318. https://doi.org/10.3390/biology10040318

Non-coding RNAs in pancreatic ductal adenocarcinoma: New approaches for better diagnosis and therapy
Mortoglou, M., Tabin, Z.K., Arisan, E.D., Kocher, H.M. and Uysal Onganer, P. 2021. Non-coding RNAs in pancreatic ductal adenocarcinoma: New approaches for better diagnosis and therapy. Translational Oncology. 14 (7) 101090. https://doi.org/10.1016/j.tranon.2021.101090

AMPK Is the Crucial Target for the CDK4/6 Inhibitors Mediated Therapeutic Responses in PANC-1 and MIA PaCa-2 Pancreatic Cancer Cell Lines
Sevgin, B., Coban, M., Özge Rencuzogullari, Coker Gurkan, A., Obakan Yerlikaya, P., Uysal Onganer, P. and Arisan, E. 2021. AMPK Is the Crucial Target for the CDK4/6 Inhibitors Mediated Therapeutic Responses in PANC-1 and MIA PaCa-2 Pancreatic Cancer Cell Lines. Stresses. 1 (1), pp. 48-68. https://doi.org/10.3390/stresses1010005

MicroRNA-Regulated Signaling Pathways: Potential Biomarkers for Pancreatic Ductal Adenocarcinoma
Mortoglou, M., Wallace, D., Buha Djordjevic, A., Vladimir Djordjevic, Arisan, E. and Uysal Onganer, P. 2021. MicroRNA-Regulated Signaling Pathways: Potential Biomarkers for Pancreatic Ductal Adenocarcinoma. Stresses. 1 (1), pp. 30-47. https://doi.org/10.3390/stresses1010004

MicroRNAs for Virus Pathogenicity and Host Responses, Identified in SARS-CoV-2 Genomes, May Play Roles in Viral-Host Co-Evolution in Putative Zoonotic Host Species
Lange, S., Arisan, E., Grant, G.H. and Uysal Onganer, P. 2021. MicroRNAs for Virus Pathogenicity and Host Responses, Identified in SARS-CoV-2 Genomes, May Play Roles in Viral-Host Co-Evolution in Putative Zoonotic Host Species. Viruses. 13 (1) 117. https://doi.org/10.3390/v13010117

MiR-21 Is Required for the Epithelial–Mesenchymal Transition in MDA-MB-231 Breast Cancer Cells
Arisan, E., Rencuzogullari, O., Clara Cieza-Borrella, Miralles Arenas, F., Miriam Dwek, Lange, S. and Uysal Onganer, P. 2021. MiR-21 Is Required for the Epithelial–Mesenchymal Transition in MDA-MB-231 Breast Cancer Cells. International Journal of Molecular Sciences. 22 (4), p. e1557 1557. https://doi.org/10.3390/ijms22041557

Peptidylarginine Deiminase Inhibitor Application, Using Cl-Amidine, PAD2, PAD3 and PAD4 Isozyme-Specific Inhibitors in Pancreatic Cancer Cells, Reveals Roles for PAD2 and PAD3 in Cancer Invasion and Modulation of Extracellular Vesicle Signatures
Uysal Onganer, P., D'alessio, S., Mortoglou, M., Kraev, I. and Lange, S. 2021. Peptidylarginine Deiminase Inhibitor Application, Using Cl-Amidine, PAD2, PAD3 and PAD4 Isozyme-Specific Inhibitors in Pancreatic Cancer Cells, Reveals Roles for PAD2 and PAD3 in Cancer Invasion and Modulation of Extracellular Vesicle Signatures. International Journal of Molecular Sciences. 22 (3) 1396. https://doi.org/10.3390/ijms22031396

MicroRNAs for Virus Pathogenicity and Host Responses, Identified in SARS-CoV-2 Genomes, May Play Roles in Viral-Host Co-Evolution in Putative Zoonotic Host Species
Lange, S., Arisan, E.D., Grant, G.H. and Uysal Onganer, P. 2021. MicroRNAs for Virus Pathogenicity and Host Responses, Identified in SARS-CoV-2 Genomes, May Play Roles in Viral-Host Co-Evolution in Putative Zoonotic Host Species. MDPI Viruses. 13 (1), p. 117 10.3390/v13010117 . https://doi.org/10.3390/v13010117

Method of Food Preparation Influences Blood Glucose Response to a High-Carbohydrate Meal: A Randomised Cross-over Trial
Hodges, C., Archer, F., Chowdhury, M., Evans, B.L., Ghelani, D.J., Mortoglou, M. and Guppy, F.M. 2020. Method of Food Preparation Influences Blood Glucose Response to a High-Carbohydrate Meal: A Randomised Cross-over Trial. Foods. 9 (1) 23. https://doi.org/10.3390/foods9010023

Elevated microRNA expression could be diagnostic biomarker for PDAC
Mortoglou, M., Arisan, D., Ferreira, T., McCormick, A.L. and Uysal Onganer, P. 2020. Elevated microRNA expression could be diagnostic biomarker for PDAC. Pancreatology. 20 (8) e19. https://doi.org/10.1016/j.pan.2020.10.015

Specific c-Jun N-Terminal Kinase Inhibitor, JNK-IN-8 Suppresses Mesenchymal Profile of PTX-Resistant MCF-7 Cells through Modulating PI3K/Akt, MAPK and Wnt Signaling Pathways
Ozfiliz Kilbas, P., Sonmez, Ozlem, Uysal Onganer, P., Coker Gurkan, A., Obakan Yerlikaya, P. and Arisan, Elif Damla 2020. Specific c-Jun N-Terminal Kinase Inhibitor, JNK-IN-8 Suppresses Mesenchymal Profile of PTX-Resistant MCF-7 Cells through Modulating PI3K/Akt, MAPK and Wnt Signaling Pathways. Biology. 9 (10), p. e320. https://doi.org/10.3390/biology9100320

Putative Roles for Peptidylarginine Deiminases in COVID-19
Arisan, E., Uysal Onganer, P. and Lange, S. 2020. Putative Roles for Peptidylarginine Deiminases in COVID-19. International Journal of Molecular Sciences. 21 (13) 4662. https://doi.org/10.3390/ijms21134662

The Prediction of miRNAs in SARS-CoV-2 Genomes: hsa-miR Databases Identify 7 Key miRs Linked to Host Responses and Virus Pathogenicity-Related KEGG Pathways Significant for Comorbidities
Arisan, E.D., Dart, A., Grant, G.H., Arisan, S., Cuhadaroglu, S., Lange, S. and Uysal Onganer, P. 2020. The Prediction of miRNAs in SARS-CoV-2 Genomes: hsa-miR Databases Identify 7 Key miRs Linked to Host Responses and Virus Pathogenicity-Related KEGG Pathways Significant for Comorbidities. MDPI Viruses. 12 (6) 614. https://doi.org/10.3390/v12060614

Protein Deimination Signatures in Plasma and Plasma-EVs and Protein Deimination in the Brain Vasculature in a Rat Model of Pre-Motor Parkinson’s Disease
Marco Sancandi, Uysal Onganer, P., Igor Kraev, Mercer, A. and Lange, S. 2020. Protein Deimination Signatures in Plasma and Plasma-EVs and Protein Deimination in the Brain Vasculature in a Rat Model of Pre-Motor Parkinson’s Disease. International Journal of Molecular Sciences. 21 (8) 2743. https://doi.org/10.3390/ijms21082743

Demonstration of microRNA using in situ hybridisation on formalin fixed paraffin wax samples using conventional oligonucleotide probes: a comparison with the use of locked nucleic acid probes
Warford, A., Rahman, N.S., Ribeiro, D.A. and Uysal Onganer, P. 2020. Demonstration of microRNA using in situ hybridisation on formalin fixed paraffin wax samples using conventional oligonucleotide probes: a comparison with the use of locked nucleic acid probes. British Journal of Biomedical Science. 77 (3), pp. 135-141. https://doi.org/10.1080/09674845.2020.1748913

The Role of Differentially Expressed miRNAs and Potential miRNA-mRNA Regulatory Network in Prostate Cancer Progression and Metastasis
Arisan, E.D., Freitas, I.L., Pazoki, K., Rahim, S. and Uysal Onganer, P. 2020. The Role of Differentially Expressed miRNAs and Potential miRNA-mRNA Regulatory Network in Prostate Cancer Progression and Metastasis. Biomedical Journal of Technical and Scientific Research. 25 (4), pp. 19382-19387. https://doi.org/10.26717/BJSTR.2020.25.004241

Upregulated wnt-11 and mir-21 expression trigger epithelial mesenchymal transition in aggressive prostate cancer cells
Arisan, E.D., Rencuzogullari, O., Freitas, I.L., Radzali, S., Keskin, B., Kothari, A., Warford, A. and Uysal-Onganer, P. 2020. Upregulated wnt-11 and mir-21 expression trigger epithelial mesenchymal transition in aggressive prostate cancer cells. Biology. 9 (3) 52. https://doi.org/10.3390/biology9030052

Deiminated Proteins and Extracellular Vesicles as Novel Biomarkers in Pinnipeds: Grey seal (Halichoerus gryptus) and Harbour seal (Phoca vitulina)
Magnadóttir, B., Uysal Onganer, P., Kraev, I., Svansson, V., Skírnisson, K. and Lange, S. 2020. Deiminated Proteins and Extracellular Vesicles as Novel Biomarkers in Pinnipeds: Grey seal (Halichoerus gryptus) and Harbour seal (Phoca vitulina). Biochimie . 171-172, pp. 79-90. https://doi.org/10.1016/j.biochi.2020.02.017

Peptidylarginine Deiminase Isozyme-Specific PAD2, PAD3 and PAD4 Inhibitors Differentially Modulate Extracellular Vesicle Signatures and Cell Invasion in Two Glioblastoma Multiforme Cell Lines
Uysal Onganer, P., Amy MacLatchy, Rayan Mahmoud, Kraev, I., Paul R. Thompson, Jameel M. Inal and Lange, S. 2020. Peptidylarginine Deiminase Isozyme-Specific PAD2, PAD3 and PAD4 Inhibitors Differentially Modulate Extracellular Vesicle Signatures and Cell Invasion in Two Glioblastoma Multiforme Cell Lines. International Journal of Molecular Sciences. 21 (4) 1495. https://doi.org/10.3390/ijms21041495

Deiminated proteins and extracellular vesicles - Novel serum biomarkers in whales and orca
Magnadóttir, B., Uysal-Onganer, P., Kraev, I., Svansson, V., Hayes, P.M. and Lange, S. 2020. Deiminated proteins and extracellular vesicles - Novel serum biomarkers in whales and orca. Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics. 34, p. 100676 100676. https://doi.org/10.1016/j.cbd.2020.100676

Extracellular Vesicles, Deiminated Protein Cargo and microRNAs are Novel Serum Biomarkers for Environmental Rearing Temperature in Atlantic Cod (Gadus morhua L.)
Magnadóttir, B., Uysal Onganer, P., Kraev, I., Dodds, A.W., Gudmundsdottir, S. and Lange, S. 2020. Extracellular Vesicles, Deiminated Protein Cargo and microRNAs are Novel Serum Biomarkers for Environmental Rearing Temperature in Atlantic Cod (Gadus morhua L.) . Aquaculture Reports. 16 100245. https://doi.org/10.1016/j.aqrep.2019.100245

Wnt-11 expression promotes invasiveness and correlates with survival in human pancreatic ductal adeno carcinoma
Dart, D.A., Arisan, D.E., Owen, S., Hao, C., Jiang, W.G. and Uysal-Onganer, P. 2019. Wnt-11 expression promotes invasiveness and correlates with survival in human pancreatic ductal adeno carcinoma. Genes. 10 (11) 921. https://doi.org/10.3390/genes10110921

Post-Translational Deimination of Immunological and Metabolic Protein Markers in Plasma and Extracellular Vesicles of Naked Mole-Rat (Heterocephalus glaber)
Matthew E. Pamenter, Uysal Onganer, P., Kenny W. Huynh, Kraev, I. and Lange, S. 2019. Post-Translational Deimination of Immunological and Metabolic Protein Markers in Plasma and Extracellular Vesicles of Naked Mole-Rat (Heterocephalus glaber). International Journal of Molecular Sciences. 20 (21) 5378. https://doi.org/10.3390/ijms20215378

Peptidylarginine Deiminases Post-translationally deiminate Prohibitin and modulate Extracellular Vesicle Release and microRNAs in Glioblastoma Multiforme
Kosgodage, U., Uysal Onganer, P., Maclatchy, A., Kraev, I., Chatterton, N.P., Nicholas, A.P., Inal, J.M. and Lange, S. 2019. Peptidylarginine Deiminases Post-translationally deiminate Prohibitin and modulate Extracellular Vesicle Release and microRNAs in Glioblastoma Multiforme . International Journal of Molecular Sciences. 20 (1) 103. https://doi.org/10.3390/ijms20010103

Cannabidiol Affects Extracellular Vesicle Release, miR21 and miR126, and Reduces Prohibitin Protein in Glioblastoma Multiforme Cells
Kosgodage, U., Uysal Onganer, P., Maclatchy, A., Mould, R., Nunn, A.V.W., Guy, G.W., Kraev, I., Chatterton, N.P., Thomas, E.L., Inal, J.M., Bell, J.D. and Lange, S. 2019. Cannabidiol Affects Extracellular Vesicle Release, miR21 and miR126, and Reduces Prohibitin Protein in Glioblastoma Multiforme Cells. Translational Oncology. 12 (3), pp. 513-522. https://doi.org/10.1016/j.tranon.2018.12.004

Prostate-specific PTen deletion in mice activates inflammatory microRNA expression pathways in the epithelium early in hyperplasia development
Dart Alwyn, D., Uysal-Onganer, P. and Jiang, W.G. 2017. Prostate-specific PTen deletion in mice activates inflammatory microRNA expression pathways in the epithelium early in hyperplasia development. Oncogenesis. 14 (6) 400. https://doi.org/10.1038/s41389-017-0007-5

The interaction of Wnt-11 and signalling cascades in prostate cancer
Koushyar, S., Grant, G.H. and Uysal-Onganer, P. 2016. The interaction of Wnt-11 and signalling cascades in prostate cancer. Tumor Biology. 37 (10), pp. 13049-13057. https://doi.org/10.1007/s13277-016-5263-z

Distinct expression and activity of GSK-3α and GSK-3β in prostate cancer
Darrington, R.S., Campa, V.M., Walker, M.M., Bengoa-Vergniory, N., Gorrono-Etxebarria, I., Uysal-Onganer, P., Kawano, Y., Waxman, J. and Kypta, R.M. 2012. Distinct expression and activity of GSK-3α and GSK-3β in prostate cancer. International Journal of Cancer. 131 (6), p. E872–E883. https://doi.org/10.1002/ijc.27620

Secreted Frizzled-related protein-1 is a negative regulator of androgen receptor activity in prostate cancer
Kawano, Y., Diez, S., Uysal-Onganer, P., Darrington, R.S., Waxman, J. and Kypta, R.M. 2009. Secreted Frizzled-related protein-1 is a negative regulator of androgen receptor activity in prostate cancer. British Journal of Cancer. 100, pp. 1165-1174. https://doi.org/10.1038/sj.bjc.6604976

Epidermal growth factor upregulates motility of Mat-LyLu rat prostate cancer cells partially via voltage-gated Na+ channel activity
Ding, Y., Brackenbury, W.J., Uysal Onganer, P., Montano, X., Porter, L.M., Bates, L.F. and Djamgoz, M.B.A. 2008. Epidermal growth factor upregulates motility of Mat-LyLu rat prostate cancer cells partially via voltage-gated Na+ channel activity. Journal of Cellular Physiology. 215 (1), pp. 77-81. https://doi.org/10.1002/jcp.21289

The expression and functional characterization of sigma (sigma) 1 receptors in breast cancer cell lines
Aydar, E., Uysal Onganer, P., Perrett, R., Djamgoz, M.B.A. and Palmer, C.P. 2006. The expression and functional characterization of sigma (sigma) 1 receptors in breast cancer cell lines. Cancer Letters. 242 (2), pp. 245-257. https://doi.org/10.1016/j.canlet.2005.11.011

An acetylcholinesterase-derived peptide inhibits endocytic membrane activity in a human metastatic breast cancer cell line
Uysal Onganer, P., Djamgoz, M.B.A., Whyte, K. and Greenfield, S.A. 2006. An acetylcholinesterase-derived peptide inhibits endocytic membrane activity in a human metastatic breast cancer cell line. Biochimica et Biophysica Acta: general subjects. 1760 (3), pp. 415-420. https://doi.org/10.1016/j.bbagen.2005.12.016

Small-cell lung cancer (human): potentiation of endocytic membrane activity by voltage-gated Na(+) channel expression in vitro
Uysal Onganer, P. and Djamgoz, M.B.A. 2005. Small-cell lung cancer (human): potentiation of endocytic membrane activity by voltage-gated Na(+) channel expression in vitro. Journal of Membrane Biology. 204, pp. 67-75. https://doi.org/10.1007/s00232-005-0747-6

Neuronal characteristics of small-cell lung cancer
Uysal Onganer, P., Seckl, M.J. and Djamgoz, M.B.A. 2005. Neuronal characteristics of small-cell lung cancer. British Journal of Cancer. 93, pp. 1197-1201. https://doi.org/10.1038/sj.bjc.6602857

Permalink - https://westminsterresearch.westminster.ac.uk/item/vq1q1/role-of-micrornas-in-response-to-cadmium-chloride-in-pancreatic-ductal-adenocarcinoma


Share this

Usage statistics

11 total views
11 total downloads
5 views this month
2 downloads this month
These values are for the period from September 2nd 2018, when this repository was created