Title | Epidermal growth factor upregulates motility of Mat-LyLu rat prostate cancer cells partially via voltage-gated Na+ channel activity |
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Type | Journal article |
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Authors | Ding, Y., Brackenbury, W.J., Uysal Onganer, P., Montano, Ximena, Porter, L.M., Bates, L.F. and Djamgoz, M.B.A. |
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Abstract | The main aim of this investigation was to determine whether a functional relationship existed between epidermal growth factor (EGF) and voltage-gated sodium channel (VGSC) upregulation, both associated with strongly metastatic prostate cancer cells. Incubation with EGF for 24 h more than doubled VGSC current density. Similar treatment with EGF significantly and dose-dependently enhanced the cells' migration through Transwell filters. Both the patch clamp recordings and the migration assay suggested that endogenous EGF played a similar role. Importantly, co-application of EGF and tetrodotoxin, a highly selective VGSC blocker, abolished 65% of the potentiating effect of EGF. It is suggested that a significant portion of the EGF-induced enhancement of migration occurred via VGSC activity. |
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Journal | Journal of Cellular Physiology |
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Journal citation | 215 (1), pp. 77-81 |
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ISSN | 1097-4652 |
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Year | 2008 |
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Publisher | Wiley |
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Accepted author manuscript | File Access Level Open (open metadata and files) |
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Publisher's version | File Access Level Open (open metadata and files) |
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Digital Object Identifier (DOI) | https://doi.org/10.1002/jcp.21289 |
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Publication dates |
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Published online | 24 Oct 2007 |
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Published in print | Apr 2008 |
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