Extracellular Vesicles, Deiminated Protein Cargo and microRNAs are Novel Serum Biomarkers for Environmental Rearing Temperature in Atlantic Cod (Gadus morhua L.)

Magnadóttir, B., Uysal Onganer, P., Kraev, I., Dodds, A.W., Gudmundsdottir, S. and Lange, S. 2020. Extracellular Vesicles, Deiminated Protein Cargo and microRNAs are Novel Serum Biomarkers for Environmental Rearing Temperature in Atlantic Cod (Gadus morhua L.) . Aquaculture Reports. 16 100245. https://doi.org/10.1016/j.aqrep.2019.100245

TitleExtracellular Vesicles, Deiminated Protein Cargo and microRNAs are Novel Serum Biomarkers for Environmental Rearing Temperature in Atlantic Cod (Gadus morhua L.)
TypeJournal article
AuthorsMagnadóttir, B., Uysal Onganer, P., Kraev, I., Dodds, A.W., Gudmundsdottir, S. and Lange, S.
Abstract

Extracellular vesicles (EVs) are released from cells and carry protein and genetic cargo involved in cell communication, as well as in apoptosis. EVs isolated from bodyfluids, including plasma and serum, can also be used as markers of pathophysiological changes. Peptidylarginine deiminases (PADs) are phylogenetically conserved enzymes with physiological and pathophysiological roles and cause post-translational protein deimination. This can affect function of target proteins and deimination is also linked to EV release. Atlantic cod (Gadus morhua L.) reared at 4 °C and 9 °C respectively for 18 months, were here assessed for changes in serum derived EVs, including analysis of deiminated protein and micro-RNA cargo markers related to stress and growth. We found that cod reared at 9 °C showed significantly reduced numbers of EVs in serum, compared to cod reared at 4 °C. Some deiminated protein targets, including complement component C3, were found to be considerably higher in EVs of cod reared at 4 °C. Proteomic analysis revealed further differences in deiminated protein targets in EVs isolated from sera of the two temperature groups. Whole cod sera from the two temperature groups furthermore showed differences in deiminated protein targets, including C3, CRP and histone H3, which is a marker of neutrophil extracellular trap formation. MicroRNAs related to inflammation (miRNA-21) and stress (miRNA-155) were elevated in both total cod serum and serum-derived EVs from the 9 °C group, while the growth-related miRNA-206 was higher in the 4 °C group. Our findings highlight EVs as novel biomarkers to assess fish health in response to environmental rearing temperature.

Keywordsextracellular vesicles (EVs); peptidylarginine deiminase (PAD); temperature; miRNA-21, miRNA-155, miRNA-206; Atlantic cod (Gadus morhua L.)
Article number100245
JournalAquaculture Reports
Journal citation16
ISSN2352-5134
Year2020
PublisherElsevier
Publisher's version
Digital Object Identifier (DOI)https://doi.org/10.1016/j.aqrep.2019.100245
Publication dates
Published online08 Nov 2019
Published in printMar 2020
LicenseCC BY-NC-ND 4.0

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Deimination Protein Profiles in Alligator mississippiensis Reveal Plasma and Extracellular Vesicle- specific Signatures Relating to Immunity, Metabolic Function and Gene Regulation
Criscitiello, M.F., Kraev, I., Petersen, L.H. and Lange, S. 2020. Deimination Protein Profiles in Alligator mississippiensis Reveal Plasma and Extracellular Vesicle- specific Signatures Relating to Immunity, Metabolic Function and Gene Regulation. Frontiers in Immunology. 11 651. https://doi.org/10.3389/fimmu.2020.00651

The Role of Differentially Expressed miRNAs and Potential miRNA-mRNA Regulatory Network in Prostate Cancer Progression and Metastasis
Arisan, E.D., Freitas, I.L., Pazoki, K., Rahim, S. and Uysal Onganer, P. 2020. The Role of Differentially Expressed miRNAs and Potential miRNA-mRNA Regulatory Network in Prostate Cancer Progression and Metastasis. Biomedical Journal of Technical and Scientific Research. 25 (4), pp. 19382-19387. https://doi.org/10.26717/BJSTR.2020.25.004241

Upregulated wnt-11 and mir-21 expression trigger epithelial mesenchymal transition in aggressive prostate cancer cells
Arisan, E.D., Rencuzogullari, O., Freitas, I.L., Radzali, S., Keskin, B., Kothari, A., Warford, A. and Uysal-Onganer, P. 2020. Upregulated wnt-11 and mir-21 expression trigger epithelial mesenchymal transition in aggressive prostate cancer cells. Biology. 9 (3) 52. https://doi.org/10.3390/biology9030052

Deiminated Proteins and Extracellular Vesicles as Novel Biomarkers in Pinnipeds: Grey seal (Halichoerus gryptus) and Harbour seal (Phoca vitulina)
Magnadóttir, B., Uysal Onganer, P., Kraev, I., Svansson, V., Skírnisson, K. and Lange, S. 2020. Deiminated Proteins and Extracellular Vesicles as Novel Biomarkers in Pinnipeds: Grey seal (Halichoerus gryptus) and Harbour seal (Phoca vitulina). Biochimie . 171-172, pp. 79-90. https://doi.org/10.1016/j.biochi.2020.02.017

Peptidylarginine Deiminase Isozyme-Specific PAD2, PAD3 and PAD4 Inhibitors Differentially Modulate Extracellular Vesicle Signatures and Cell Invasion in Two Glioblastoma Multiforme Cell Lines
Uysal Onganer, P., Amy MacLatchy, Rayan Mahmoud, Kraev, I., Paul R. Thompson, Jameel M. Inal and Lange, S. 2020. Peptidylarginine Deiminase Isozyme-Specific PAD2, PAD3 and PAD4 Inhibitors Differentially Modulate Extracellular Vesicle Signatures and Cell Invasion in Two Glioblastoma Multiforme Cell Lines. International Journal of Molecular Sciences. 21 (4) 1495. https://doi.org/10.3390/ijms21041495

Deiminated proteins and extracellular vesicles - Novel serum biomarkers in whales and orca
Magnadóttir, B., Uysal-Onganer, P., Kraev, I., Svansson, V., Hayes, P.M. and Lange, S. 2020. Deiminated proteins and extracellular vesicles - Novel serum biomarkers in whales and orca. Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics. 34, p. 100676 100676. https://doi.org/10.1016/j.cbd.2020.100676

Protein Deimination and Extracellular Vesicle Profiles in Antarctic Seabirds
Phillips, R.A., Kraev, I. and Lange, S. 2020. Protein Deimination and Extracellular Vesicle Profiles in Antarctic Seabirds. MDPI Biology. 9 (1) 15. https://doi.org/10.3390/biology9010015

Plasma mEV levels in Ghanain malaria patients with low parasitaemia are higher than those of healthy controls, raising the potential for parasite markers in mEVs as diagnostic targets
Antwi-Baffour, S., Malibha-Pinchbeck, M., Stratton, D., Jorfi, S., Lange, S. and Inal, J.M. 2020. Plasma mEV levels in Ghanain malaria patients with low parasitaemia are higher than those of healthy controls, raising the potential for parasite markers in mEVs as diagnostic targets. Journal of Extracellular Vesicles. 9, 2020 (issue 1) 1697124 . https://doi.org/10.1080/20013078.2019.1697124

Deiminated Proteins in Extracellular Vesicles and Serum of Llama (Lama glama) - Novel Insights into Camelid Immunity
Criscitiello, M.F., Kraev, I. and Lange, S. 2020. Deiminated Proteins in Extracellular Vesicles and Serum of Llama (Lama glama) - Novel Insights into Camelid Immunity. Molecular Immunology. 117, pp. 37-53 MIMM_5890. https://doi.org/10.1016/j.molimm.2019.10.017

Peptidylarginine Deiminase Inhibition Abolishes the Production of Large Extracellular Vesicles From Giardia intestinalis, Affecting Host-Pathogen Interactions by Hindering Adhesion to Host Cells
Gavinho, B., Sabatke, B., Feijoli, V., Rossi, I., da Silva, J. M., Evans-Osses, I., Palmisano. G., Lange, S. and Ramirez, M.I. 2020. Peptidylarginine Deiminase Inhibition Abolishes the Production of Large Extracellular Vesicles From Giardia intestinalis, Affecting Host-Pathogen Interactions by Hindering Adhesion to Host Cells. Frontiers in Cellular and Infection Microbiology. 10 417. https://doi.org/10.3389/fcimb.2020.00417

Special Issue: Peptidylarginine Deiminases and Protein Deimination in Health and Disease
Lange, S. and Nicholas, A.P. 2019. Special Issue: Peptidylarginine Deiminases and Protein Deimination in Health and Disease. MDPI.

Wnt-11 expression promotes invasiveness and correlates with survival in human pancreatic ductal adeno carcinoma
Dart, D.A., Arisan, D.E., Owen, S., Hao, C., Jiang, W.G. and Uysal-Onganer, P. 2019. Wnt-11 expression promotes invasiveness and correlates with survival in human pancreatic ductal adeno carcinoma. Genes. 10 (11) 921. https://doi.org/10.3390/genes10110921

Post-Translational Deimination of Immunological and Metabolic Protein Markers in Plasma and Extracellular Vesicles of Naked Mole-Rat (Heterocephalus glaber)
Matthew E. Pamenter, Uysal Onganer, P., Kenny W. Huynh, Kraev, I. and Lange, S. 2019. Post-Translational Deimination of Immunological and Metabolic Protein Markers in Plasma and Extracellular Vesicles of Naked Mole-Rat (Heterocephalus glaber). International Journal of Molecular Sciences. 20 (21) 5378. https://doi.org/10.3390/ijms20215378

Curcumin: novel treatment in neonatal hypoxic-ischaemic brain injury
Rocha-Ferreira, E., Sisa, C., Bright, S., Fautz, T., Harris, M., Contreras Riquelme, I., Agwu, C., Kurulday, T., Mistry, B., Hill, D., Lange, S. and Hristova, M. 2019. Curcumin: novel treatment in neonatal hypoxic-ischaemic brain injury. Frontiers in Physiology. 10 1351. https://doi.org/10.3389/fphys.2019.01351

Cannabidiol is a Novel Modulator of Bacterial Membrane Vesicles
Kosgodage, U., Matewele, P., Awamaria, B., Kraev, I., Warde, P., Mastroianni, G., Nunn, A., Guy, G.W., Bell, J.D., Inal, J.M. and Lange, S. 2019. Cannabidiol is a Novel Modulator of Bacterial Membrane Vesicles. Frontiers in Cellular and Infection Microbiology. 9 324. https://doi.org/10.3389/fcimb.2019.00324

Complement component C4-like protein in Atlantic cod (Gadus morhua L.) - Detection in ontogeny and identification of post-translational deimination in serum and extracellular vesicles
Lange, S., Kraev, I., Magnadottir, B. and Dodds, A.W. 2019. Complement component C4-like protein in Atlantic cod (Gadus morhua L.) - Detection in ontogeny and identification of post-translational deimination in serum and extracellular vesicles. Developmental and Comparative Immunology. 101 103437. https://doi.org/10.1016/j.dci.2019.103437

Deiminated Proteins in Extracellular Vesicles and Plasma of Nurse Shark (Ginglymostoma cirratum) - Novel Insights into Shark Immunity
Criscitiello, M.F., Kraev, I. and Lange, S. 2019. Deiminated Proteins in Extracellular Vesicles and Plasma of Nurse Shark (Ginglymostoma cirratum) - Novel Insights into Shark Immunity. Fish and Shellfish Immunology. 92, pp. 249-255 YFSIM_6208. https://doi.org/10.1016/j.fsi.2019.06.012

Peptidylarginine Deiminase Inhibitors Reduce Bacterial Membrane Vesicle Release and Sensitise Bacteria to Antibiotic Treatment
Kosgodage, U., Matewele, P., Mastroianni, G., Kraev, I., Brotherton, D., Awamaria, B., Nicholas, A.P., Lange, S. and Inal, J.M. 2019. Peptidylarginine Deiminase Inhibitors Reduce Bacterial Membrane Vesicle Release and Sensitise Bacteria to Antibiotic Treatment. Frontiers in Cellular and Infection Microbiology. 9 227. https://doi.org/10.3389/fcimb.2019.00227

Extracellular Vesicles from Cod (Gadus morhua L.) Mucus contain Innate Immune Factors and Deiminated Protein Cargo
Magnadóttir, B., Kraev, I., Gudmundsdottir, S., Dodds, A.W. and Lange, S. 2019. Extracellular Vesicles from Cod (Gadus morhua L.) Mucus contain Innate Immune Factors and Deiminated Protein Cargo. Developmental and Comparative Immunology. p. 103397 103397. https://doi.org/10.1016/j.dci.2019.103397

Mesenchymal Stromal Cell derived Extracellular Vesicles reduce Hypoxia-Ischaemia Induced Perinatal Brain Injury
Sisa, C., Kholia, S., Naylor, J., Herrera Sanchez, M.B., Bruno, S., Deregibus, M.C., Camussi, G., Inal, J.M., Lange, S. and Hristova, M. 2019. Mesenchymal Stromal Cell derived Extracellular Vesicles reduce Hypoxia-Ischaemia Induced Perinatal Brain Injury. Frontiers in Physiology. 10 282. https://doi.org/10.3389/fphys.2019.00282

Peptidylarginine Deiminases Post-translationally deiminate Prohibitin and modulate Extracellular Vesicle Release and microRNAs in Glioblastoma Multiforme
Kosgodage, U., Uysal Onganer, P., Maclatchy, A., Kraev, I., Chatterton, N.P., Nicholas, A.P., Inal, J.M. and Lange, S. 2019. Peptidylarginine Deiminases Post-translationally deiminate Prohibitin and modulate Extracellular Vesicle Release and microRNAs in Glioblastoma Multiforme . International Journal of Molecular Sciences. 20 (1) 103. https://doi.org/10.3390/ijms20010103

A novel ladder-like lectin relates to sites of mucosal immunity in Atlantic Halibut (Hippoglossus hippoglossus L.)
Magnadóttir, B., Gudmundsdottir, S. and Lange, S. 2019. A novel ladder-like lectin relates to sites of mucosal immunity in Atlantic Halibut (Hippoglossus hippoglossus L.) . Fish & Shellfish Immunology. 87, pp. 9-12. https://doi.org/10.1016/j.fsi.2018.12.034

Cannabidiol Affects Extracellular Vesicle Release, miR21 and miR126, and Reduces Prohibitin Protein in Glioblastoma Multiforme Cells
Kosgodage, U., Uysal Onganer, P., Maclatchy, A., Mould, R., Nunn, A.V.W., Guy, G.W., Kraev, I., Chatterton, N.P., Thomas, E.L., Inal, J.M., Bell, J.D. and Lange, S. 2019. Cannabidiol Affects Extracellular Vesicle Release, miR21 and miR126, and Reduces Prohibitin Protein in Glioblastoma Multiforme Cells. Translational Oncology. 12 (3), pp. 513-522. https://doi.org/10.1016/j.tranon.2018.12.004

Peptidylarginine deiminase and deiminated proteins are detected throughout early halibut ontogeny - Complement components C3 and C4 are post-translationally deiminated in halibut (Hippoglossus hippoglossus L.)
Magnadóttir, B., Bragason, B.T., Bricknell, I.R., Bowden, T., Nicholas, A.P., Hristova, M., Gudmundsdottir, S., Dodds, A.W. and Lange, S. 2019. Peptidylarginine deiminase and deiminated proteins are detected throughout early halibut ontogeny - Complement components C3 and C4 are post-translationally deiminated in halibut (Hippoglossus hippoglossus L.). Developmental and Comparative Immunology. 92, pp. 1-19. https://doi.org/10.1016/j.dci.2018.10.016

The physiological variability of channel density in hippocampal CA1 pyramidal cells and interneurons explored using a unified data-driven modeling workflow
Migliore, R., Lupascu, C.A., Bologna, L.L., Romani, R., Courcol, J-D., Antonel, S., Van Geit, W.A.H., Thompson, A.M., Mercer, A., Lange, S., Falck, J., Rossert, C.A., Shi, Y., Hagens, O., Pezzoli, M., Freund, T.F., Muller, E.B., Kali, S., Schurmann, F., Markram, H. and Migliore, M. 2018. The physiological variability of channel density in hippocampal CA1 pyramidal cells and interneurons explored using a unified data-driven modeling workflow. PLOS Computational Biology. 14 (9) e1006423. https://doi.org/10.1371/journal.pcbi.1006423

Cannabidiol (CBD) is a Novel Inhibitor for Exosome and Microvesicle (EMV) Release in Cancer
Kosgodage, U., Mould, R., Henley, A., Nunn, A.V.W., Guy, G.W., Thomas, E.L., Inal, J.M., Bell, J.D. and Lange, S. 2018. Cannabidiol (CBD) is a Novel Inhibitor for Exosome and Microvesicle (EMV) Release in Cancer. Frontiers in Pharmacology. 9 889. https://doi.org/10.3389/fphar.2018.00889

Post-translational Protein Deimination in Cod (Gadus morhua L.) Ontogeny: Novel Roles in Tissue Remodelling and Mucosal Immune Defences?
Magnadóttir, B., Hayes, P.M., Hristova, M., Bragason, B., Nicholas, A.P., Dodds, A.W., Gudmundsdottir, S. and Lange, S. 2018. Post-translational Protein Deimination in Cod (Gadus morhua L.) Ontogeny: Novel Roles in Tissue Remodelling and Mucosal Immune Defences? Developmental and Comparative Immunology. 87, pp. 157-170. https://doi.org/10.1016/j.dci.2018.06.006

Pentraxins CRP-I and CRP-II are post-translationally deiminated and differ in tissue specificity in cod (Gadus morhua L.) ontogeny
Magnadóttir, B., Hayes, P.M., Gisladottir, B., Bragason, B., Hristova, M., Nicholas, A.P., Gudmundsdottir, S. and Lange, S. 2018. Pentraxins CRP-I and CRP-II are post-translationally deiminated and differ in tissue specificity in cod (Gadus morhua L.) ontogeny. Developmental and Comparative Immunology. 87, pp. 1-11. https://doi.org/10.1016/j.dci.2018.05.014

Prostate-specific PTen deletion in mice activates inflammatory microRNA expression pathways in the epithelium early in hyperplasia development
Dart Alwyn, D., Uysal-Onganer, P. and Jiang, W.G. 2017. Prostate-specific PTen deletion in mice activates inflammatory microRNA expression pathways in the epithelium early in hyperplasia development. Oncogenesis. 14 (6) 400. https://doi.org/10.1038/s41389-017-0007-5

Data-driven computational modeling of CA1 hippocampal principal cells and interneurons
Lange, S., migliore, S. and Lupascu, A.C. 2017. Data-driven computational modeling of CA1 hippocampal principal cells and interneurons. 26th Annual Computational Neuroscience Meeting (CNS*2017). Antwerp, Belgium 15 - 20 Jul 2017 BioMed Central. https://doi.org/10.1186/s12868-017-0372-1

Treatment of Prostate Cancer Using Deimination Antagonists and Microvesicle Technology
Lange, S., Kholia, S., Kosgodage, U. and Inal, J.M. 2017. Treatment of Prostate Cancer Using Deimination Antagonists and Microvesicle Technology. in: Nicholas, A.P., Bhattacharya, S. and Thompson, P.R. (ed.) Protein Deimination in Human Health and Disease Cham, Switzerland Springer. pp. 413-425

Protein Deimination in Protein Misfolding Disorders - Modelled in Human induced Pluripotent Stem Cells (iPSCs)
Lange, S., Wray, S., Devine, M., Matarin, M. and Hardy, J. 2017. Protein Deimination in Protein Misfolding Disorders - Modelled in Human induced Pluripotent Stem Cells (iPSCs). in: Nicholas, A.P., Battacharya, S.K. and Thompson, P.R. (ed.) Protein Deimination in Human Health and Disease Cham, Switzerland Springer. pp. 227-239

Peptidylarginine Deiminases-Roles in Cancer and Neurodegeneration and Possible Avenues for Therapeutic Intervention via Modulation of Exosome and Microvesicle (EMV) Release?
Lange, S., Gallagher, M, Kholia, S., Kosgodage, U., Hristova, M., Hardy, J. and Inal, J.M. 2017. Peptidylarginine Deiminases-Roles in Cancer and Neurodegeneration and Possible Avenues for Therapeutic Intervention via Modulation of Exosome and Microvesicle (EMV) Release? International Journal of Molecular Sciences. 18 (6) 1196. https://doi.org/10.3390/ijms18061196

Chloramidine/Bisindolylmaleimide-I-Mediated Inhibition of Exosome and Microvesicle Release and Enhanced Efficacy of Cancer Chemotherapy
Kosgodage, U., Trindade R.P., Thompson, P.R., Inal, J.M. and Lange, S. 2017. Chloramidine/Bisindolylmaleimide-I-Mediated Inhibition of Exosome and Microvesicle Release and Enhanced Efficacy of Cancer Chemotherapy. International Journal of Molecular Sciences. 18 (5) 1007. https://doi.org/10.3390/ijms18051007

The emerging role of exosome and microvesicle- (EMV-) based cancer therapeutics and immunotherapy
Moore, C., Kosgodage, U., Lange, S. and Inal, J.M. 2017. The emerging role of exosome and microvesicle- (EMV-) based cancer therapeutics and immunotherapy. International Journal of Cancer. 141 (3), pp. 428-436. https://doi.org/10.1002/ijc.30672

Data-driven model of the hippocampus using the HBP Brain Simulation Platform
Romani, A., Antille, N., Atenekeng, G., Courcol, J.D., Devresse, A., Dynes, J.A., Gevaert, M., Gonzalo, J.K., Gulyas, A., Kali, S., Kanari, L., Lange, S., Mercer, A., Migliore, M., Muller, E.B., Palacios, J.P., Ramaswamy, S., Reimann, M., Riquelme, R.L., Rössert, C.A., Ying, S., Shillcock, J., Telefont, M., Van Geit, W.A.H., Vanherpe, L., Markram, H. and Thomson, A. 2016. Data-driven model of the hippocampus using the HBP Brain Simulation Platform. 10th FENS Forum of Neuroscience. Copenhagen, Denmark 02 - 06 Jul 2016

The interaction of Wnt-11 and signalling cascades in prostate cancer
Koushyar, S., Grant, G.H. and Uysal-Onganer, P. 2016. The interaction of Wnt-11 and signalling cascades in prostate cancer. Tumor Biology. 37 (10), pp. 13049-13057. https://doi.org/10.1007/s13277-016-5263-z

PF7.09 Non-phagocytic epithelial cells take up microvesicles by micropinocytosis
Lange, S., Kosgodage, U. and Inal, J.M. 2016. PF7.09 Non-phagocytic epithelial cells take up microvesicles by micropinocytosis. The Fifth International Meeting of ISEV, ISEV 2016. Rotterdam, Netherlands 04 - 07 May 2016 Co-Action Publishing.

PW1.04. Inhibitors of microvesicle release with the potential to enhance effectivity of cancer chemotherapy
Lange, S., Kosgodage, U. and Inal, J.M. 2016. PW1.04. Inhibitors of microvesicle release with the potential to enhance effectivity of cancer chemotherapy. The Fifth International Meeting of ISEV, ISEV 2016. Rotterdam, Netherlands 04 - 07 May 2016 Co-Action Publishing. https://doi.org/10.3402/jev.v5.31552

Peptidylarginine deiminases as drug targets in neonatal hypoxic-ischemic encephalopathy
Lange, S. 2016. Peptidylarginine deiminases as drug targets in neonatal hypoxic-ischemic encephalopathy. Frontiers in Neurology. 7 22. https://doi.org/10.3389/fneur.2016.00022

Prostate cancer cells stimulated by calcium-mediated activation of protein kinase C undergo a refractory period before re-releasing calcium-bearing microvesicles
Lange, S., Stratton, D., Moore, C., Inal, J., Zheng, L. and Li, Z. 2015. Prostate cancer cells stimulated by calcium-mediated activation of protein kinase C undergo a refractory period before re-releasing calcium-bearing microvesicles. Biochemical and Biophysical Research Communications. 460 (3), pp. 511-517. https://doi.org/10.1016/j.bbrc.2015.03.061

Microvesicles released constitutively from prostate cancer cells differ biochemically and functionally to stimulated microvesicles released through sublytic C5b-9
Lange, S., Stratton, D., Moore, C., Antwi-Baffour, S. and Inal, J. 2015. Microvesicles released constitutively from prostate cancer cells differ biochemically and functionally to stimulated microvesicles released through sublytic C5b-9. Biochemical and Biophysical Research Communications. 460 (3), p. 589–595. https://doi.org/10.1016/j.bbrc.2015.03.074

Data-driven Hippocampus CA1 Modeling in the Human Brain Project
Romani, A., Antille, N., Mercer, A., Dynes, J.A., Falk, J., Gevaert, M., Gonzalo, J.K., Kali, S., Kanari, L., Migliore, M., Lange, S., Muller, E.B., Ramaswamy, S., Reimann, M., Riquelme, R.J.L., Rössert, C.A., Shi, Y., Telefont, M., Van Geit, W.A.H., Markram, H., Thomson, A. and W.A.H. Van Geit 2015. Data-driven Hippocampus CA1 Modeling in the Human Brain Project. Neuroscience 2015. Chicago 17 Oct 2015 Society for Neuroscience.

A novel role for peptidylarginine deiminases in microvesicle release reveals therapeutic potential of PAD inhibition in sensitizing prostate cancer cells to chemotherapy
Kholia, S., Jorfi, S., Thompson, P.R., Causey, C.P., Nicholas, A.P., Inal, J. and Lange, S. 2015. A novel role for peptidylarginine deiminases in microvesicle release reveals therapeutic potential of PAD inhibition in sensitizing prostate cancer cells to chemotherapy. Journal of Extracellular Vesicles. 4 (1) 26192. https://doi.org/10.3402/jev.v4.26192

Inhibition of microvesiculation sensitizes prostate cancer cells to chemotherapy and reduces docetaxel dose required to limit tumor growth in vivo
Jorfi, S., Ansa-Addo, E.A., Kholia, S., Stratton, D., Valley, S., Lange, S. and Inal, J. 2015. Inhibition of microvesiculation sensitizes prostate cancer cells to chemotherapy and reduces docetaxel dose required to limit tumor growth in vivo. Scientific Reports. 5 (13006) 13006. https://doi.org/10.1038/srep13006

Label-free real-time acoustic sensing of microvesicle release from prostate cancer (PC3) cells using a Quartz Crystal Microbalance
Lange, S., Stratton, D., Kholia, S., Jorfi, S., Antwi-Baffour, S. and Inal, J. 2014. Label-free real-time acoustic sensing of microvesicle release from prostate cancer (PC3) cells using a Quartz Crystal Microbalance. Biochemical and Biophysical Research Communications. 453 (3), p. 619–624. https://doi.org/10.1016/j.bbrc.2014.09.132

Peptidylarginine deiminases (PADs): novel drug targets for prevention of neuronal damage following hypoxic ischemic insult (HI) in neonates
Lange, S., Rocha-Ferreira, E., Thei, L., Mawjee, P., Thompson, P.R., Nicholas, A.P., Hristova, M., Peebles, D. and Raivich, G. 2014. Peptidylarginine deiminases (PADs): novel drug targets for prevention of neuronal damage following hypoxic ischemic insult (HI) in neonates. Journal of Neurochemistry. 130 (4), pp. 555-562. https://doi.org/10.1111/jnc.12744

The Role of Infection and the TNF Family of Cytokines in Neonatal Hypoxic-Ischemic Brain Injury
Rocha-Ferreira, E., Francesch-Domenech, E., Thei, L., Rahim, A., Lange, S., Peebles, D., Hristova, M. and Raivich, G. 2014. The Role of Infection and the TNF Family of Cytokines in Neonatal Hypoxic-Ischemic Brain Injury. Reproductive Sciences. 21 (3 suppl), pp. 108A-109A. https://doi.org/10.1177/1933719114528275

Pulsed extremely low-frequency magnetic fields stimulate microvesicle release from human monocytic leukaemia cells
Lange, S., Stratton, D. and Inal, J. 2013. Pulsed extremely low-frequency magnetic fields stimulate microvesicle release from human monocytic leukaemia cells. Biochemical and Biophysical Research Communications. 430 (2), pp. 470-475. https://doi.org/10.1016/j.bbrc.2012.12.012

The Role of Deimination as a Response to Trauma and Hypoxic Injury in the Developing CNS
Ferretti, P., Lange, S., U, KP and Raivich, G. 2013. The Role of Deimination as a Response to Trauma and Hypoxic Injury in the Developing CNS. in: Nicholas, A. and Bhattacharya, S. (ed.) Protein Deimination in Human Health and Disease Springer. pp. 281-294

Blood/plasma secretome and microvesicles
Inal, J.M., Kosgodage, U., Azam, S., Stratton, D., Antwi-Baffour, S. and Lange, S. 2013. Blood/plasma secretome and microvesicles. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 1834 (11), pp. 2317-2325. https://doi.org/10.1016/j.bbapap.2013.04.005

Interplay of host–pathogen microvesicles and their role in infectious disease
Inal, J.M., Ansa-Addo, E.A. and Lange, S. 2013. Interplay of host–pathogen microvesicles and their role in infectious disease. Biochemical Society Transactions. 41 (1), pp. 258-262. https://doi.org/10.1042/BST20120257

Inhibition of Protein Deimination Ameliorates Neuronal Damage Caused by Hypoxic Ischemic Insult in the Neonatal Brain
Lange, S., Rocha-Ferreira, E., Hristova, M., Thei, L., Mawjee, P., Thompson, P.R., Nicholas, T. and Raivich, G. 2013. Inhibition of Protein Deimination Ameliorates Neuronal Damage Caused by Hypoxic Ischemic Insult in the Neonatal Brain. British Neuroscience Association Abstracts. 22, pp. P1-B-071.

Distinct expression and activity of GSK-3α and GSK-3β in prostate cancer
Darrington, R.S., Campa, V.M., Walker, M.M., Bengoa-Vergniory, N., Gorrono-Etxebarria, I., Uysal-Onganer, P., Kawano, Y., Waxman, J. and Kypta, R.M. 2012. Distinct expression and activity of GSK-3α and GSK-3β in prostate cancer. International Journal of Cancer. 131 (6), p. E872–E883. https://doi.org/10.1002/ijc.27620

Microvesicles in Health and Disease
Inal, J.M., Ansa-Addo, E.A., Stratton, D., Kholia, S., Antwi-Baffour, S., Jorfi, S. and Lange, S. 2012. Microvesicles in Health and Disease. Archivum Immunologiae et Therapiae Experimentalis. 60 (2), pp. 107-121. https://doi.org/10.1007/s00005-012-0165-2

TNF gene deletion prevents lipopolysaccharide-mediated sensitisation of the neonatal mouse brain to hypoxic-ischaemic insult
Sahota, A., Kendall, G., Lange, S. and Raivich, G. 2012. TNF gene deletion prevents lipopolysaccharide-mediated sensitisation of the neonatal mouse brain to hypoxic-ischaemic insult. BMC Proceedings. 6 (Suppl 4), p. O9. https://doi.org/10.1186/1753-6561-6-S4-O9

Characterisation of microvesicles released from cells constitutively and upon stimulation
Stratton, D., Antwi-Baffour, S., Williams, G., Grant, R., Lange, S. and Inal, J.M. 2012. Characterisation of microvesicles released from cells constitutively and upon stimulation. Journal of Extracellular Vesicles. 1.

Isolation of microvesicles and exosomes by filtration and estimation of normal reference range in blood plasma
Grant, R., Ansa-Addo, E., Stratton, S., Antwi-Baffour, S., Jorfi, S., Kholia, S., Krige, L., Lange, S. and Inal, J.M. 2012. Isolation of microvesicles and exosomes by filtration and estimation of normal reference range in blood plasma. Journal of Extracellular Vesicles. 1.

The intracellular parasite, Trypanosoma cruzi, utilizes microvesicle release to invade host cells
Inal, J.M., Ansa-Addo, E., Cestari, I., Pathak, P.I., McCrossan, M.V., Lange, S. and Ramirez, M. 2012. The intracellular parasite, Trypanosoma cruzi, utilizes microvesicle release to invade host cells. Journal of Extracellular Vesicles. 1.

A filtration-based protocol to isolate human plasma membrane-derived vesicles and exosomes from blood plasma
Lange, S., Grant, R., Ansa-Addo, E.A., Stratton, D., Antwi-Baffour, S., Jorfi, S., Kholia, S., Krige, L. and Inal, J. 2011. A filtration-based protocol to isolate human plasma membrane-derived vesicles and exosomes from blood plasma. Journal of Immunological Methods. 371 (1-2), pp. 143-151. https://doi.org/10.1016/j.jim.2011.06.024

Protein deiminases: new players in the developmentally regulated loss of neural regenerative ability
Lange, S., Gogel, S., Yeung K-Y, Nicholas, A.P., Causey, C.P., Thompson, P.R., Greene, N.D.E. and Ferretti, P. 2011. Protein deiminases: new players in the developmentally regulated loss of neural regenerative ability. Developmental Biology. 355 (2), pp. 205-214. https://doi.org/10.1016/j.ydbio.2011.04.015

Post-translational regulation of CRMP in developing and regenerating chick spinal cord
Lange, S., Gogel, S., Leung Kit-Y, Greene, N.D.E. and Ferretti, P. 2010. Post-translational regulation of CRMP in developing and regenerating chick spinal cord. Developmental Neurobiology. 70 (6), pp. 456-471. https://doi.org/10.1002/dneu.20789

Human plasma membrane-derived vesicles inhibit the phagocytosis of apoptotic cells: possible role in SLE
Lange, S., Antwi-Baffour, S., Kholia, S., Ansa-Addo, E.A., Stratton, D., Aryee Y, K. and Inal, J. 2010. Human plasma membrane-derived vesicles inhibit the phagocytosis of apoptotic cells: possible role in SLE. Biochemical and Biophysical Research Communications. 398 (2), pp. 278-283. https://doi.org/10.1016/j.bbrc.2010.06.079

Human plasma membrane-derived vesicles halt proliferation and induce differentiation of THP-1 acute monocytic leukemia cells
Lange, S., Ansa-Addo, E.A., McCrossan, M.V. and Inal, J. 2010. Human plasma membrane-derived vesicles halt proliferation and induce differentiation of THP-1 acute monocytic leukemia cells. Journal of Immunology. 185 (9), pp. 5236-5246. https://doi.org/10.4049/jimmunol.1001656

Red cell PMVs, plasma membrane-derived vesicles calling out for standards
Hind, E., Heugh, S., Ansa-Addo, E.A., Antwi-Baffour, S., Lange, S. and Inal, J.M. 2010. Red cell PMVs, plasma membrane-derived vesicles calling out for standards. Biochemical and Biophysical Research Communications. 399 (4), pp. 465-469. https://doi.org/10.1016/j.bbrc.2010.07.095

Spinal cord regeneration: A role for peptidylarginine deiminase (PAD) in secondary injury response
Lange, S. 2010. Spinal cord regeneration: A role for peptidylarginine deiminase (PAD) in secondary injury response. SfN Minisymposium on Deimination in Opthalmic and Neurodegenerative diseases. San Diego Convention Center, California 16 Nov 2010

Spinal cord regeneration: peptidylarginine deiminase (PAD) and epigentic regulation of injury response
Lange, S., Ferretti, P., Goegel, S., Leung, K.T., Nicholas, A.P., Thompson, P.R. and Greene, N.D.E. 2010. Spinal cord regeneration: peptidylarginine deiminase (PAD) and epigentic regulation of injury response. FENS Forum 2010. Amsterdam 04 Jul 2010 Federation of European Neuroscience Societies.

Secreted Frizzled-related protein-1 is a negative regulator of androgen receptor activity in prostate cancer
Kawano, Y., Diez, S., Uysal-Onganer, P., Darrington, R.S., Waxman, J. and Kypta, R.M. 2009. Secreted Frizzled-related protein-1 is a negative regulator of androgen receptor activity in prostate cancer. British Journal of Cancer. 100, pp. 1165-1174. https://doi.org/10.1038/sj.bjc.6604976

Changes in progenitor populations and ongoing neurogenesis in the regenerating chick spinal cord
Lange, S., Whalley, K., Gogel, S. and Ferretti, P. 2009. Changes in progenitor populations and ongoing neurogenesis in the regenerating chick spinal cord. Developmental Biology. 332 (2), p. 234–245. https://doi.org/10.1016/j.ydbio.2009.05.569

Epidermal growth factor upregulates motility of Mat-LyLu rat prostate cancer cells partially via voltage-gated Na+ channel activity
Ding, Y., Brackenbury, W.J., Uysal Onganer, P., Montano, X., Porter, L.M., Bates, L.F. and Djamgoz, M.B.A. 2008. Epidermal growth factor upregulates motility of Mat-LyLu rat prostate cancer cells partially via voltage-gated Na+ channel activity. Journal of Cellular Physiology. 215 (1), pp. 77-81. https://doi.org/10.1002/jcp.21289

Proteome changes in developing and injured chick spinal cord
Goegel, S., Lange, S., Greene, N.D.E. and Ferretti, P. 2007. Proteome changes in developing and injured chick spinal cord. Neuron Glia Biology. pp. S122-S122.

Differential gene expression following chick spinal cord injury at permissive and non permissive stages for regeneration
Lange, S., Goegel, S., Greene, N.D.E. and Ferretti, P. 2007. Differential gene expression following chick spinal cord injury at permissive and non permissive stages for regeneration. Neuron Glia Biology. pp. S121-S121.

The complement regulatory receptor CRIT is expressed in the developing kidney
Lange, S., Hui Kwok-M, Schifferli, J. and Inal, J.M. 2007. The complement regulatory receptor CRIT is expressed in the developing kidney. Molecular Immunology. 44 (16), p. 3991. https://doi.org/10.1016/j.molimm.2007.06.213

Complement C2 and CRIT are involved in the terminal differentiation of monocytes
Inal, J.M., Eva, W., Lange, S., Shamshiev, A. and Schifferli, J. 2007. Complement C2 and CRIT are involved in the terminal differentiation of monocytes. Journal of Immunology. 178, p. 83.17.

The expression and functional characterization of sigma (sigma) 1 receptors in breast cancer cell lines
Aydar, E., Uysal Onganer, P., Perrett, R., Djamgoz, M.B.A. and Palmer, C.P. 2006. The expression and functional characterization of sigma (sigma) 1 receptors in breast cancer cell lines. Cancer Letters. 242 (2), pp. 245-257. https://doi.org/10.1016/j.canlet.2005.11.011

An acetylcholinesterase-derived peptide inhibits endocytic membrane activity in a human metastatic breast cancer cell line
Uysal Onganer, P., Djamgoz, M.B.A., Whyte, K. and Greenfield, S.A. 2006. An acetylcholinesterase-derived peptide inhibits endocytic membrane activity in a human metastatic breast cancer cell line. Biochimica et Biophysica Acta: general subjects. 1760 (3), pp. 415-420. https://doi.org/10.1016/j.bbagen.2005.12.016

Immunostimulation of larvae and juveniles of cod, Gadus morhua L.
Lange, S., Magnadóttir, B., Gudmundsdóttir, B.K., Bambir, S.H., Steinarsson, A., Oddgeirsson, M., Bowden, T., Bricknell, I., Dalmo, R.A. and Gudmundsdottir, S. 2006. Immunostimulation of larvae and juveniles of cod, Gadus morhua L. Journal of Fish Diseases. 29 (3), p. 147–155. https://doi.org/10.1111/j.1365-2761.2006.00701.x.

CRIT is expressed on podocytes in normal human kidney and upregulated in membranous nephropathy
Lange, S., Moll, S., Mihatsch M, J., Schifferli, J. and Inal, J. 2006. CRIT is expressed on podocytes in normal human kidney and upregulated in membranous nephropathy. Kidney International. 69 (11), p. 1961–1968. https://doi.org/10.1038/sj.ki.5000379

Complement component C3 transcription in Atlantic halibut (Hippoglossus hippoglossus L.) larvae
Lange, S., Bambir, S.H., Dodds, A.W., Bowden, T., Bricknell, I., Espelid, S. and Magnadóttir, B. 2006. Complement component C3 transcription in Atlantic halibut (Hippoglossus hippoglossus L.) larvae. Fish & Shellfish Immunology. 20 (3), p. 285–294. https://doi.org/10.1016/j.fsi.2005.05.004

Small-cell lung cancer (human): potentiation of endocytic membrane activity by voltage-gated Na(+) channel expression in vitro
Uysal Onganer, P. and Djamgoz, M.B.A. 2005. Small-cell lung cancer (human): potentiation of endocytic membrane activity by voltage-gated Na(+) channel expression in vitro. Journal of Membrane Biology. 204, pp. 67-75. https://doi.org/10.1007/s00232-005-0747-6

Neuronal characteristics of small-cell lung cancer
Uysal Onganer, P., Seckl, M.J. and Djamgoz, M.B.A. 2005. Neuronal characteristics of small-cell lung cancer. British Journal of Cancer. 93, pp. 1197-1201. https://doi.org/10.1038/sj.bjc.6602857

Complement C2 receptor inhibitor trispanning: a novel human complement inhibitory receptor
Lange, S., Inal, J., Hui Kwok-M, Miot, S., Ramirez, M., Schneider, B. and Krueger, G. 2005. Complement C2 receptor inhibitor trispanning: a novel human complement inhibitory receptor. The Journal of Immunology. 174 (1), pp. 356-366.

The ontogenic transcription of complement component C3 and Apolipoprotein A-I tRNA in Atlantic cod (Gadus morhua L.): a role in development and homeostasis?
Lange, S., Dodds, A.W., Bambir, S.H., Gudmundsdottir, S. and Magnadóttir, B. 2005. The ontogenic transcription of complement component C3 and Apolipoprotein A-I tRNA in Atlantic cod (Gadus morhua L.): a role in development and homeostasis? Developmental & Comparative Immunology. 29 (12), p. 1065–1077. https://doi.org/10.1016/j.dci.2005.03.009

The Complement Systems of Two Teleost Species with Emphasis on Ontogeny
Lange, S. 2005. The Complement Systems of Two Teleost Species with Emphasis on Ontogeny. PhD thesis University of Iceland Faculty of Medicine

Ontogeny of humoral immune parameters in fish
Magnadóttir, B., Lange, S., Gudmundsdottir, S., Bogwald, J. and Dalmo, R.A. 2005. Ontogeny of humoral immune parameters in fish. Fish and Shellfish Immunology. 19 (5), pp. 429-439. https://doi.org/10.1016/j.fsi.2005.03.010

The ontogeny of complement component C3 in Atlantic cod (Gadus morhua L.)—an immunohistochemical study
Lange, S., Bambir, S., Dodds, A.W. and Magnadóttir, B. 2004. The ontogeny of complement component C3 in Atlantic cod (Gadus morhua L.)—an immunohistochemical study. Fish & Shellfish Immunology. 16 (3), pp. 359-367. https://doi.org/10.1016/j.fsi.2003.06.001

Is Apolipoprotein A-I a regulating protein for the complement system of cod (Gadus morhua L.)?
Magnadóttir, B. and Lange, S. 2004. Is Apolipoprotein A-I a regulating protein for the complement system of cod (Gadus morhua L.)? Fish and Shellfish Immunology. 16 (2), pp. 265-269. https://doi.org/10.1016/S1050-4648(03)00061-5

Isolation and characterization of complement component C3 from Atlantic cod (Gadus morhua L.) and Atlantic halibut (Hippoglossus hippoglossus L.)
Lange, S., Dodds, A.W. and Magnadóttir, B. 2004. Isolation and characterization of complement component C3 from Atlantic cod (Gadus morhua L.) and Atlantic halibut (Hippoglossus hippoglossus L.). Fish and Shellfish Immunology. 16 (2), pp. 227-239. https://doi.org/10.1016/S1050-4648(03)00081-0

Spontaneous haemolytic activity of Atlantic halibut (Hippoglossus hippoglossus L.) and sea bass (Dicentrarchus labrax) serum
Lange, S. and Magnadóttir, B. 2003. Spontaneous haemolytic activity of Atlantic halibut (Hippoglossus hippoglossus L.) and sea bass (Dicentrarchus labrax) serum. Comparative Biochemistry and Physiology - Part B: Biochemistry & Molecular Biology. 136 (1), pp. 99-106. https://doi.org/10.1016/S1096-4959(03)00173-8

Protection against atypical furunculosis in Atlantic halibut, Hippoglossus hippoglossus (L.); comparison of a commercial furunculosis vaccine and an autogenous vaccine
Gudmundsdottir, S., Lange, S., Magnadóttir, B. and Gudmundsdóttir, B.K. 2003. Protection against atypical furunculosis in Atlantic halibut, Hippoglossus hippoglossus (L.); comparison of a commercial furunculosis vaccine and an autogenous vaccine. Journal of Fish Diseases. 26 (6), pp. 331-338. https://doi.org/10.1046/j.1365-2761.2003.00462.x

Humoral immune parameters of cultured Atlantic halibut (Hippoglossus hippoglossus L.)
Lange, S., Guđmundsdottir, B.K. and Magnadóttir, B. 2001. Humoral immune parameters of cultured Atlantic halibut (Hippoglossus hippoglossus L.). Fish and Shellfish Immunology. 11 (6), pp. 523-535. https://doi.org/10.1006/fsim.2000.0333

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