Title | Prostate cancer cells stimulated by calcium-mediated activation of protein kinase C undergo a refractory period before re-releasing calcium-bearing microvesicles |
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Authors | Lange, S., Stratton, D., Moore, C., Inal, J., Zheng, L. and Li, Z. |
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Abstract | MVs are released in response to several stress agents, in an attempt to prevent continued cellular damage. After an initial stimulus of prostate cancer cells with sublytic C5b-9 and activation of MV release through PKC, cells take at least 20 min to fully recover their ability to microvesiculate. This release of MVs through activation of sublytic C5b-9 was inhibited by the PKC inhibitor bisindoylmaleimide I but not the Rho kinase inhibitor, Y27632. After stimulus there is a rise of 79 nMs(-1) over 11 s, reaching a peak [Ca(2+)]i of 920 nM. The concentration of cytosolic calcium then falls steadily at 2.4 nMs(-1) over 109 s reaching baseline levels (50-100 nM) within 10-15 min. In PC3 cells the rate of release of MVs from stimulated cells also reaches a minimum within 10-15 min. Using fura-2 AM-loaded cells, upon stimulation, cells were found to release MVs with a concentration of intravesicular calcium estimated at ∼ 430 nM. |
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Keywords | Microvesicles; Refractory period; Sublytic C5b-9; Intravesicular calcium |
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Journal | Biochemical and Biophysical Research Communications |
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Journal citation | 460 (3), pp. 511-517 |
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ISSN | 0006-291X |
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Year | 2015 |
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Publisher | Elsevier |
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Digital Object Identifier (DOI) | https://doi.org/10.1016/j.bbrc.2015.03.061 |
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Publication dates |
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Published | 08 May 2015 |
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